Syntheses, Characterizations and Properties of Cucurbit[n]uril and its Coordination Complexes

摘要瓜环(cucurbit[n]uril)是超分子化学中继冠醚、环糊精、杯芳烃之后备受瞩目的一类新型笼状大环主体分子化合物。由于其特有的结构特性和广阔的应用前景，瓜环受到国内外相关学科研究者的关注。目前，人们对瓜环的合成、主客体性质（分子识别、分子开关、具有特殊结构分子器件的组装和超分子组装）进行了广泛而深入的研究，并且在瓜环的应用方面取得了巨大的进展。本论文在文献调研的基础上，首先在第一章就瓜环及其同系物、衍生物和同类物的合成、结构等方面的一些基本性质，瓜环在分子识别、分子容器、分子开关与超分子组装方面的主客体性质，瓜环的应用研究的最新进展进行了全方位的综述。其次，本论文系统地考察了Q5，Q...Abstract Cucurbit[n]uril (Qn) family, as a new type of macrocycle molecular host, has been the subject of much interest in recent years because of its structural specialties and its potential applications as functional materials. The synthesis and the fundamental properties of Qn have been delineated by the pioneering work of the research groups of Mock, Kim, and Day, and have led to their applica...学位：理学博士院系专业：化学化工学院化学系_无机化学学号：B20042500

Interesting anion-inclusion behavior of Cucurbit[5]uril and its lanthanide-capped molecular capsule

通讯作者地址: Long, LS (通讯作者), Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surface, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, Dept Chem, State Key Lab Phys Chem Solid Surface, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] selective encapsulation behavior of cucurbit [5]uril and its lanthanide-capped molecular capsule toward nitrate and chloride ions has been investigated. Using fluorescence spectroscopy, the metal-free host has been demonstrated to selectively include nitrate ion. In contrast, the lanthanicle-capped molecular capsule showed preference toward the inclusion of chloride ion. Solid-state structures of the nitrate and chloride inclusion complexes with the La(III)-capped molecular capsule have been crystallographically determine

From 1D zigzag chain to 1D tubular structure, weak field ligand-dependent assembly of cucurbit[6]uril-based tubular coordination polymer

通讯作者地址: Long, LS (通讯作者), Xiamen Univ, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surface, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] reaction of cucurbit[6]uril with Cu(NO3)(2) and CuCl2, respectively, generates a 1D zigzag chain of {[Cu(H2O)(4)( cucurbit[6]uril)]center dot(NO3)(2)center dot(H2O)(8)}(n) and a 1D tubular structure of {[Cu(H2O)(2)(Cl)(2)(cucurbit[6]uril)](1/3)center dot(H2O)(3)}(n), showing clearly how to control the reversible and mutual interconnections between ligand and metal ion under the guide of coordination chemistry.NNSFC 20825103 20721001 973 project 2007CB815304 Natural Science Foundation of Fujian Province of China 2008J001

端面反射对SG-DBR激光器性能的影响

通过传输矩阵模型,理论研究了不同端面反射的存在对取样光栅分布Bragg反射(SG-DBR)镜以及集成半导体放大器(SOA)的SG-DBR镜反射谱的影响,进而研究了端面反射的存在对SG-DBR激光器以及集成SOA的激光器模式的影响,结果表明,端面反射的存在会使取样光栅的各级反射峰由1个分裂为2个甚至多个,并可能使边峰的强度高过零级峰,这将会严重恶化SG-DBR激光器及其集成SOA的激光器模式特性和调谐特性.理论分析与实验结果基本吻合.当端面的反射率小于0.01时,端面反射对于SG-DBR激光器的影响可以忽略不

Preparation and Cryo-EM Structure Determination of Human Papillomavirus 16 Pseudovirion Derived from Suspension-adapted HEK293 Cells

为了大量获得人乳头瘤病毒(HPV)16型的假病毒颗粒(PsV),将贴壁生长的293FT细胞驯化成为悬浮细胞系进行大规模的悬浮培养,同时优化假病毒纯化方法,并使用冷冻电镜解析其三维空间结构。本研究通过逐步降低培养基中血清浓度和加入抗聚团剂等方法,获得了可在Wave生物反应器中大规模悬浮培养的293FT细胞,经过携有HPV16L1、L2基因和GFP报告基因的质粒转染,实现了大规模制备HPV16假病毒;利用CsCl等密度和蔗糖密度梯度超速离心的方法,获得高纯度的HPV16假病毒颗粒,滴度为8.2×10~5 TCID50/!L;蛋白印迹结果显示HPV16假病毒中含有L1和L2蛋白,定量ELISA测得L1蛋白的含量为156.0μg/mL;最后使用Tecnai G2F30冷冻电镜和Falcon电子直接探测相机采集冷冻HPV16假病毒的图像,应用AUTO3DEM软件解析其结构,结构分辨率为14,呈现为T=7的等二十面体对称结构,直径为600。本研究为HPV疫苗临床血清中和检测、高分辨率HPV16假病毒的结构解析及L1和L2相互作用研究奠定了良好基础。The goals of this study were to establish a scalable production method to prepare human papillomavirus(HPV)16pseudovirus(PsV)using suspension-adapted HEK-293 FT cells and to improve the purification efficiency of HPV PsV.Furthermore,we aimed to solve the cryo-electron microscopy(cryo-EM)structure of HPV16 PsV.The suspension f HEK-293 FT cells were generated from adherent cells by a stepwise decrease in serum content and the addition of an anti-clumping agent during culturing.The resultant HEK-293 FT suspension cells were transfected with an L1/L2 expression vector and pN31-EGFP plasmid to generate HPV16 PsV in the Wave Bioreactor.Following cell lysis,HPV16 PsV was purified by sucrose density gradient and subsequent CsCl iso-density gradient ultra-centrifugation The final titer of HPV16 PsV was 8.2 × 105 TCID50/!L.Purified HPV16 PsV was comfirmed to as contain L1 and L2protein by western blotting,and the L1 concentration was determined to be 156.0μg/mL by quantitative ELISA.Finally,a FEI Tecnai G2F30 electron microscope and AUTO3 DEM were used to solve the cryoEM structure of HPV16 PsV at a resolution of 14.The structure shows that HPV16 PsV exists as a T=7dicosahedral lattice,with a diameter of 600.These results will be beneficial for neutralization assays and for anti-sera for HPV vaccines,the high-resolution structure determination of HPV16 PsV,and the investigation of interactions between HPV L1 and L2.国家自然科学基金(项目号:81172885),题目:基于中和表位结构的高位HPV型交叉疫苗的分子设