The Study on Decoction of Kangxin Capsule and Its Minor Components are Influenced on Peripheral Blood CD_3、CD_4、CD_8 and CD_4/CD_8 of the Kidney Weakness with Blood Obstruction Style Vascular Dementia Rat Model

目的 　观察康欣胶囊及其拆方对 ( VD)模型 (肾虚血瘀型 )大鼠外周血 CD3、CD4、CD8的影响。 方法 　采用电灼烧封闭双侧椎动脉和摘除单侧性器官法制作 ( VD)模型 (肾虚血瘀型 )大鼠 ,用康欣胶囊全方及其拆方和西药喜得镇给药 1个月 ,电镜、光镜观察各组大鼠海马、下丘脑、枕部皮质神经细胞情况 ,流式细胞仪检测大鼠外周血 CD3、CD4、CD8。 结果 　康欣胶囊全方组海马、下丘脑、枕部皮质神经细胞的内织网、线粒体水肿固缩及细胞核固缩情况明显轻于模型空白对照组 ;外周血 CD4/ CD8比例指标明显高子空白模型组、健脾组、西药组 ( P<0 .0 1) ,养血活血组 CD4/ CD8比例指标高于空白模型组、健脾组 ( P<0 .0 5 )。结论 　康欣胶囊全方及其拆方养血活血方可提高 ( VD)模型 (肾虚血瘀型 )大鼠外周血 CD4/ CD8比例水平即提高免疫力 ,并延缓减轻缺血性神经细胞凋亡。OBJECTIVE To observe decoction of KANGXIN capsule and its minor components are influenced peripheral blood CD3、CD4、CD8 of the kidney weakness with blood obstruction style vascular dementia rat model.METHOD Use electric iron to burn and block the both side vertebral arteries and amputate one side genital organ(testis or ovary) of rat, made the kidney weakness with blood obstruction style of VD rat model.KANGXIN capsule complete components and its minor components: and Dinydroergotaxine had been given for l month,at the same time to establish the control model group (hadn't given any medication),complete components, kidney tonic, pancreas tonic, blood tonic with promoting blood circulation, and western medicine Dihydroergotaxine group. Use electronic microscopy and light microscopy to observe nerve cells in hippocampus, hypothalamus, occipital lobe cortex of each group, and use cell cytometry to analysis the peripheral blood CD3,CD4,CD8 of model rat.RESULTS Under electronic microscopy;the mitochondrion, rough endoplasmic reticulum and nuclear of nerve cells of hippocampus,hypothalamus,occipital lobe cortex of complete components group of KANGXIN capsule are slighter swelling and reduce (other groups had improved with difference scale), those are compared with control model group. Peripheral blood CD4/CD8 rate of Complete component group are higher than control model, pancreas tonic, and Dihydroergotaxine group,(P<0.01),CD4/CD8 rate of blood tonic with promoting blood circulation group are higher than control moodel and pancreas tonic group(P<0.05).CONCLUSION KANGXIN capsule and blood tonic with promoting blood circulation group are able to enhance immunity(lift up the CD4/CD8 rate) of the kidney weakness with blood obstruction style VD rat, and to slow down apoptosis of nerve cells which is caused by vertebrobasilar insufficiency.福建省卫生厅重大中医药项目 ( 2 0 0 2 -10 3