Dieulafoy病的诊断与治疗(附9例病例分析)

目的　探讨Dieulafoy病的临床特点诊断及治疗方法。方法　回顾性分析9例Dieu lafoy病的临床表现、诊断、治疗方法。结果　均为上消化道大出血。胃镜确诊8例。手术确诊1例,胃镜下治愈7例。手术治愈2例。结论　提高对本病认识,急诊胃镜是首选诊断方法。内镜下止血为首选治疗方法,反复出血应及时转外科手术

Expression of the Fused Pili and Ovine IL-2 Gene in Pseudomonasaeruginus

用改进的 Mg Cl2 法制备宿主菌株PAK/ 2 pfs感受态细胞 ;由 JM10 5工程菌中提取纤毛蛋白与绵羊白细胞介素 2 ( Pilin-IL-2 )融合基因表达载体 ,转化宿主细胞 PAK/ 2 pfs,筛选出具有 Pilin-IL-2融合基因表达载体的阳性克隆菌株 ;在营养肉汤中进行 Pilin-IL-2融合基因的表达。培养 16～ 18h后 ,离心 ,向上清液中加入饱和 ( NH4) 2 SO4提取 Pilin-IL-2融合基因表达蛋白。用羊抗兔 E型节瘤拟杆菌抗血清与提取的融合蛋白进行对流免疫电泳 ,证明该融合蛋白具有特异性The pmPilin IL-2 was transformed into the host competent cell PAK/rpfs and the recombinant pmPilin IL-2 was expressed in the supernatant of the culture of the transformed cell PAK/rpfs.The recombinant pmPilin IL-2 was purified by (NH_4)_2_SO_4 from the supernatant of the culture of the transformed PAK/rpfs. The specific reaction of the recombinant pmPilin IL-2 and antiserum of B.nodosus serotype E pilin was demonstrated by cross electrophoresis.国家科技部研究所技术开发研究专项资金项目[国科财字(1999) 592号

Protective Antigens from Virulent Isolates of Fusobacterium necrophorum FN(A)

通过胰蛋白酶裂解坏死梭杆菌FN(A)型毒力菌株菌体,分别以菌体裂解物上清和沉淀作为抗原免疫家兔制备血清。利用SDS-PAGE/Western-blot技术在菌体中筛选出4种具有免疫原性的组分,通过免疫后的攻毒试验,筛选出1种具有免疫保护性的抗原。抗原分离纯化后,进行N端氨基酸序列测定、免疫试验及生物学特性研究,据试验结果可初步判定该抗原为溶血素类似物或一种新发现的抗原物质。该抗原不仅能使机体产生保护性免疫反应,而且不同菌株中此种抗原间可产生明显的交叉免疫。A virulent isolate of the bacterium FN(A) was lysed with trypsin.Supernatants and precipitates of the bacterial lysates were used to prepare anti-FN serum by immunizing rabbits.Four immunogenic components were isolated using SDS-PAGE/Western-blot.Immunized rabbits were challenged,and one protective antigen was screened.After antigen isolation and purification,the N-terminal amino acid sequence was determined and an immunological test and hemolysis test were performed.Results suggested that this antigen is either a hemolysin analogue or a new antigenic substance.The antigen was able to induce a protective immune response as well as generate a marked cross-immune reaction among different bacterial strains.国家科技部奶牛专项(2002BA518A04);; 中国农业科学院特产研究所科研基金项目(tcs-20041

Spatial distribution and correlation of environmental factors andchlorophyll a concentrations in the Bohai Sea during the summer of 2013

通过对2013年7月渤海海域26个站点温度、盐度、营养盐及叶绿素a(Chl a)浓度的空间分布特征及其相关性进行了分析,发现:受渤海水深和夏季陆源河流输入影响,近岸水域表现出明显的高温、低盐与高营养盐特征,且垂直变化特征不显著;在水深较深的辽东湾湾口和渤海海峡,海水呈现明显的层化现象,表层水温高于中底层,而表层盐度与营养盐浓度则低于中底层。营养盐结构分析表明,渤海夏季磷酸盐浓度存在显著的绝对与相对限制,而受河流输入影响,硅酸盐的相对限制得到显著缓解。表层Chl a浓度的高值区位于滦河及复州河河口附近海区,中层与底层的高值区则出现在滦河与黄河口附近。Chl a浓度与环境因子的相关性分析表明,盐度..

Expression and Identification of the fused Dichelobacter Pili and Ovine Interleukin 2 Gene in Pseudomonasaeruginus

将前期工作中筛选出的含有腐蹄病节瘤拟杆菌纤毛蛋白与绵羊白细胞介素-2(Pilin-IL-2)融合基因表达载体的PAK/2pfs工程菌[1]接种于营养肉汤,40℃培养16～18h,离心,向上清液中加入饱和(NH4)2SO4提取Pilin-IL-2融合基因表达蛋白。用羊抗兔E型节瘤拟杆菌抗血清与提取的融合蛋白进行对流免疫电泳,证明该融合蛋白具有特异性,且有较高的表达量。经SDS-PAGE电泳和Westernblot确证其为Pilin-IL-2融合蛋白。The pmPilinIL2 was transformed into the host competent cell PAK/rpfs and the recombinant pmPilinIL2 was expressed in the supernatant of the coltures of the transformant cell PAK/rpfs.The recombinant pmPilinIL2 was purified by (NH_4)_2SO_4 from the supernatant of the colture of the transformed PAK/rpfs. The specificreaction of the recombinant pmPilinIL2 and antiserum of D.nodosus serotype E pilin was demonstrated by cross electrophoresis.33000 Expression protein was found by SDS-PAGE.Through Western-biotting,the expression protein was recognized by antiserum of D.nodosus serotype E pilin.国家科技部研究所技术开发研究专项资金项目[国科财字(1999)592号

Cloning and Construction Expressing Vector of Ovine Interleukin-2 Gene

从绵羊血中分离白细胞,提取绵羊白细胞RNA,利用RT-PCR、PCR扩增绵羊白细胞介素2(OvineIL-2)基因。将OvineIL-2PCR产物与TE载体定向连接,用SaLI和BamHI双酶切重组TE,利用低溶点胶回收500bp大小的片段;将其补平后与PPSD质粒连接,用EcoRI酶切鉴定,找出正向连接重组质粒。用BamHI酶切重组PPSD质粒,低熔点胶回收2500bp大小的片段;将其与经BamHI酶切去磷酸化的PME290表达质粒连接,筛选出阳性重组PME290,即为OvineIL-2基因表达载体。IL2 is known to be as an adjuvant in many areas. The ovine IL2 gene with synthesized recognition sequence at 5` and 3` end respectivecy was amplified and cloned from the peripheral blood of ovine. Ovine IL2 gene was amplified with ovine RNA as template by RTPCR and PCR. The amplified fragment was subjected to restriction digest and coined into the TE and PPSD vector. An expression plasmid was constructed by cloning the IL2 gene into PME290. The PME290SDIL2 was transformed into the host competent cell PAK/2pfs and the recombinant IL2 was expressed in the supernatant of the cultures of the transformant cell PAK/2pfs.国家科技部科研院所技术开发专项资金项目(国科发财字(1999)592号

Cloning and Expression of D.nodosus Serotype C Pili Gene in Pseudomonas

用 PCR从腐蹄病 C型节瘤拟杆菌扩增出具有免疫保护性抗原 0 .85 kb纤毛蛋白基因 (pili基因 ) ,并构建了该基因的表达载体。转化宿主细胞 PAK/2 pfs,在营养肉汤中进行 pili基因的表达。培养 18～ 2 4h后 ,收集培养液上清 ,加入 0 .1mol/L Mg Cl2 ,离心提取重组纤毛蛋白。用羊抗兔 C型节瘤拟杆菌抗血清与提取的纤毛蛋白进行对流免疫电泳试验 ,结果表达的重组纤毛蛋白有特异性 ;用 SDS- PAGE和 Western- blot证明表达的蛋白是 C型节瘤拟杆菌纤毛蛋白。The pili gene that dominates the main protective immunogen was amplified and cloned from D.nodosus serorype C by PCR.An expression plasmid was constructed by cloning the pili gene into PME290.The plasmid harbouring the pili sequence was designated PME290-pili.The PME290-pili was transformed into the host competent cell PAK/2pfs and the recombinant pili was expressed in the supernatant of the cultures of the transformant cell PAK/2pfs.The recombinant pili was purified by MgCl 2 from the supernatant of the culture of the transformed PAK/2pfs.The specific reacion of the recombinant pili and antiserum of D.nodosus serotype C pili was demonstrated by cross electrophoresis.The recombinant pili was expressed at high level in PAK/2pfs.国家科技部研究所技术开发研究专项基金项目〔国科财字 (1999) 5 92号

Impact of land reclamation on marine hydrodynamic and ecological environment

近10年来,中国海岸带围填海活动呈现出规模大、速度快的发展态势。围填海能带来显著的经济效益,但对海洋环境与生态的负面影响也不可忽视。针对围填海对海洋环境和生态的影响及作用机制,分别从水动力和生态系统两个方面进行了概述。围填海改变了海洋的自然几何属性(原始岸线、地形地貌、海湾面积),引起水动力环境的变化(潮汐系统和海湾水交换能力),进而影响了海湾的环境容量;围填海破坏了生物栖息地、导致生物多样性的丧失,影响到生态系统结构与功能的稳定性;水动力与生物多样性的变化可显著影响到生物地球化学过程,加速富营养化进程,恶化水质,增加生态灾害风险。目前,围填海后的生态修复策略主要有增加生物量、建设自然保护区、..

Spatio-Temporal Distribution of Nitrogen and Phosphorus and Spatial and Multi-Variable Statistical Analysis of Its Impacting Factors in Xiaoqinghe Watershed ---Environmental indication of macroalgal δ15N values at intertidal zone

通过对烟台潮间带大型海藻组织中总碳(TC)、总氮(TN)、氮稳定同位素(δ15N)和水环境参数的分析,对氮营养盐来源进行了判定,并筛选了合适的指示性藻种。烟台潮间带大型海藻的δ15N值均位于6.18‰~10.99‰之间,指示了生活污水与养殖排放是海水氮营养盐的主要来源。其中月亮湾(S1)主要受生活污水影响,养马岛(S3)受养殖排放影响更明显,而渔人码头(S2)同时受生活污水和养殖排放影响。绿藻门的孔石莼(Ulva pertusa)和肠浒苔(Enteromorpha intestinalis)对氮营养盐有较好的吸收和贮存能力,同时在利用氮营养盐的过程中分馏作用不明显,较适合于作为氮营养盐来源的指..

Acid treatment effects on the carbon stable isotope values of marine sediments

海洋沉积物中有机碳同位素(δ13C)可以示踪海洋生态系统中有机质来源,对环境研究具有重要意义。分析沉积物中有机物的δ13C,需要对样品进行酸化,以去除无机碳的影响。由于不同来源的沉积物中无机碳的含量和组份存在差异,需要针对样品性质,优化酸化处理过程。本研究分别选取了无机碳含量不同的温带与热带河口、海湾沉积物样品,比较了3种不同酸化过程对有机物δ13C分析的影响。研究结果表明:方法1(酸洗法)中6%H_2SO_3和1mol/L H_3PO_4对无机碳含量较高的热带河口、海湾样品去除效率较低,而2mol/L HCl去除无机碳酸盐的效果较理想。方法2(酸蒸法)并不适用于无机碳含量较高的热带河口、海湾..