超声波法乙醇提取山楂叶黄酮的研究

目前,山楂叶中有效成分黄酮的提取主要使用的乙醇提取法,该法存在提取温度高、时间长等缺点,本研究在超声波条件下,采用正交实验设计考察了乙醇浸提山楂叶中黄酮的提取工艺条件。广西科学基金项目(桂科基:0236037)； 广西教育厅项目(桂教科研[2002]316号)资

Capillary Electrophoretic Separation of Enantiomorphs of Shikimic Acid

采用毛细管电泳分离莽草酸及其对映体。试验了缓冲溶液的种类、浓度、pH值、分离电压、进样时间、添加剂对分离的影响。结果表明:以30mmol·L-1三(羟甲基)氨基甲烷-30mmol·L-1 H3BO3(pH8.6)为缓冲体系,在波长214nm,分离电压为15kV,进样时间为10s的条件下,实现了对莽草酸及其对映体的分离。High performance capillary electrophoresis was applied to the separation of enantiomorphs of shikimic acid. Several important factors influential to the separation i. e- pH and concentration of the running buffer solution, working voltage of separation, time of sample introduction and the use of additives, were studied and optimized. Enantiomorphs of shikimic acid were separated satisfactorily under the follwing optimized conditions： （2） running buffer solution： 30 mmol·L^-1 Tris·HCl＋30 mmol·L^-1 H3BO3 （pH 8. 6）; （2） working voltage of separation.. 15 kV; （4） wavelength of UV-detection： 214 nm; （4） time of sample introduction： 10 s.广西自然科学基金项目(桂科自:0236037； 桂科自:0640038)资

溴麝香草酚蓝比色法测定叶下珠生物碱的含量

目的测定叶下珠中总生物碱的含量。方法以溴麝香草酚蓝为显色剂于415 nm波长处测定吸光度。结果苦参碱在0.004~0.02 mg/m l范围内线性关系良好(r=0.997 54);其与溴麝香草酚蓝所形成的离子对产物在6 h内稳定;平均加样回收率为98.65%(RSD=1.98%)。结论该方法简单、快速、准确,可用于生药及产品的生物碱质量控制。广西自然科学基金项目资助(No.0640038

Determination of rutin in phyllanthus urinaria L. by high performance capillary electrophoresis

采用高效毛细管电泳法对叶下珠中芦丁的含量进行测定。研究了缓冲液的种类、浓度、pH、电泳运行电压、进样时间等因素对分离检测的影响。结果表明,以60 mmol硼酸盐(pH 9.0)为缓冲液,16 mmol/L的SDS为添加剂,检测波长为245 nm,分离电压为15 kV,进样时间15 s,芦丁在20~500 mg/L线性关系良好(r=0.998),回收率为98.9%,RSD为2.1%,该法操作简单,回收率及重现性良好。A method of high performance capillary electrophoresis was established for the detection of rutin in phyllanthus urinaria L. Effects of several important factors such as the pH and eoncentration of running buffer, concentration of SDS, operating voltage, and injection time, were investigated. Under the optimized condition using 60 mmol/L of Na2BO4 ＋ 15 mmol of SDS （pH 9.0） as the running buffer at an operating voltage of 15 kV and a detection wavelength of 245 nm,the concentration of rutin demonstrated good linear relationship with the peak height in the range of 20 - 500 mg/L （ r = 0.998 9）, with the average recovery rate reaching 98.9% and RSD equating to 2.1%.广西省科学基金项目(桂科自:0640038)资