Identification and Biological Characteristics Analysis of a Wild Pleurotus pulmonarius

本研究以野外采集获得的1株野生侧耳属真菌为对象,对其进行rDNA-ITS序列扩增测序并分析后初步确定其为肺形侧耳(Pleurotus; pulmonarius),并与本实验室中现有的秀珍菇菌株序列进行随机扩增多态性DNA; (RAPD)、ISSR、相关序列扩增多态性(SRAP)的综合分析后进一步确定该野生菌株与其他菌株间具有一定的遗传距离,是一株新的秀珍菇菌株。以福; 建省认定的秀珍菇品种秀迪1号为对照菌株,对该菌株进行生物学特性进行研究,结果表明,其菌丝可在温度为5~45°C的范围内生长,最适生长温度为30°; C;菌丝可生长的pH范围为pH; 3~10,最适pH值为7。在含可溶性淀粉、酵母粉为碳氮源的培养基中,菌丝生长速度快,长势好。该野生菌株生长所需的最适碳、氮源及生长条件与对照菌株; 相似,这为其进一步的驯化栽培提供一定的理论基础。In this study, a wild pleurotus strain collected in the forest was; selected as the research object. We sequenced its rDNA-ITS sequence and; analyzed the result to preliminarily classified it as Pleurotus; pulmonarius. Also, the comparision of the random amplified polymorphic; DNA (RAPD), ISSR and sequence related amplified polymorphism (SRAP) with; the strain sequence of other available Pleurotus geesteranus in our; laboratory showed that this strain had certain genetic distance with; other tested strains, which indicated that it was a new Pleurotus; geesteranus strain. The Pleurotus pulmonarius No.l approved by Fujian; province was selected as control group, and its biological; characteristics was analyzed, the result of which showed that its; mycelium could grow at a temperature of 5~45℃, and the optimum growth; temperature was 30°C. The pH range of the mycelium growth was 3 to 10,; and the optimum pH value was 7. In the substrate containing soluble; starch and yeast powder as carbon and nitrogen source, the mycelium grew; fast and good. The most suitable carbon, nitrogen sources and growth; conditions of this wild strain were similar to the control strain, which; provided a theoretical basis for the further domestication and; cultivation of this strain.福州市农业科技项