可德兰多糖及其衍生物的生物活性和应用研究进展

可德兰多糖是一种不含侧链分支的微生物发酵型β-1，3-葡聚糖，具有特殊的凝胶性质，除作为食品添加剂外，在水产养殖也有着广泛的应用。多糖分子的聚合度、分子构象和分支程度与生物活性密切相关，通过化学改性得到的可德兰多糖衍生物不仅使得溶解性增加，而且其生物活性也有了明显的提高。同时，化学修饰后的可德兰多糖也具有开发为一种新型基因载体的广泛应用前景。本文综述了可德兰多糖及其衍生物的生物活性作用机理及其应用研究进展

β-1,3-glucan oligosaccharides triggered quicker defense reactions than high degree of polymerization in tobacco

Chemically hydrolyzed curdlan oligosaccharides (CRDO), low degree of polymerization (DP) of β-1,3-glucan, were prepared to compare with Laminaran (Lam) with high DP for discussing the difference on defense-inducing activity in tobacco. Fast response defenses such as extracellular pH shift, H2O2 production, nitric oxide （NO） release and stomatal movement under light as well as the protection against tobacco mosaic virus (TMV) were performed. CRDO triggered faster extracellular alkalinization than Lam. Refractory states analysis showed there existed different perception modes between β-1,3-glucan and chitosan oligosaccharides (COS). Elicitors induced cells to generate NO probably via nitric oxide synthase (NOS) pathway. Moreover, CRDO exhibited more intensive than Lam in stomatal movement. However, protection against TMV implied Lam played a longer term role than CRDO in defense responses. Therefore, we concluded β-1,3-glucan with low DP played a vital role in rapid responses, whereas high DP β-1,3-glucan displayed durable effects.Chemically hydrolyzed curdlan oligosaccharides (CRDO), low degree of polymerization (DP) of β-1,3-glucan, were prepared to compare with Laminaran (Lam) with high DP for discussing the difference on defense-inducing activity in tobacco. Fast response defenses such as extracellular pH shift, H2O2 production, nitric oxide （NO） release and stomatal movement under light as well as the protection against tobacco mosaic virus (TMV) were performed. CRDO triggered faster extracellular alkalinization than Lam. Refractory states analysis showed there existed different perception modes between β-1,3-glucan and chitosan oligosaccharides (COS). Elicitors induced cells to generate NO probably via nitric oxide synthase (NOS) pathway. Moreover, CRDO exhibited more intensive than Lam in stomatal movement. However, protection against TMV implied Lam played a longer term role than CRDO in defense responses. Therefore, we concluded β-1,3-glucan with low DP played a vital role in rapid responses, whereas high DP β-1,3-glucan displayed durable effects

壳寡糖快速诱导烟草细胞胞外pH碱化的研究

本文考查不同寡糖激发子对烟草细胞胞外pH碱化的影响，证明了壳寡糖能快速高效诱导胞外pH碱化，同时，植物对于壳寡糖的再次诱导不存在不应期

一种燃料电池耗氢技术用于提高扁藻光解海水产氢的方法

本发明涉及绿藻光解水制氢，具体地说是，以具有光解海水产氢功能的海洋绿藻—亚心形扁藻为体系产氢材料，在光反应器上外接碱性氢氧燃料电池持续耗氢系统来维持体系内较低氢分压，依据电路中电子数量与氢原子数的一一对应关系，通过测量电池外电路电流变化来反映体系内氢浓度的变化，研究扁藻在解偶联剂CCCP调控下连续光照和非连续光照产氢过程的特征。在燃料电池持续耗氢下，细胞密度为8×106 cells ml-1，非连续光照(L/D＝9h∶6h)比连续光照产氢时间延长3倍，为120h；氢气产量提高1.6倍，为126ml l-1，且产氢过程中pH值、PSII光化学活性、体外氢酶活性变化有一定的对应关系。本发明实现了对产氢过程高效、灵敏的实时检测，方法简便，在产氢机理和工程基础研究中具有广泛应用前景。带填

一种可德兰寡糖的应用

本发明公开了一种可德兰寡糖的应用，其结构为葡萄糖分子通过β-(1→3)糖苷键连接而成的寡糖，聚合度(Degree?of?Polymerization，DP)为2-24，分子式是(C6H12O6)n，n＝2-24，所述的可德兰寡糖的平均分子量在340-4000Da。本发明制备的可德兰寡糖可作为调节植物生长、诱导植物抗性的绿色生物农用剂。待填

可德兰寡糖的制备及其组分分析

目的：可德兰多糖是目前发现的唯一不含分支的线性β-1,3-葡聚糖,由于难溶于水而限制其生物活性的研究,为此对可德兰寡糖的制备及其组分进行研究。方法：采用化学法酸解可德兰多糖。结果：通过红外光谱与基质辅助激光解析/电离飞行时间质谱（MALDI-TOF MS）的检测,结果表明：可德兰多糖降解后结构未发生变化,降解产物的聚合度为2～19,其中聚合度为5的成分含量最高;高效阴离子交换色谱脉冲安培检测法分析结果表明：反应温度105℃、反应时间150～180min是降解可德兰寡糖的合理反应条件,降解效果最佳。结论：本研究建立的方法可简单、高效的降解可德兰多糖,制备其寡糖