The regulation of AKT activity by CDK5 via EGFR phosphorylation pathway

细胞周期蛋白依赖性激酶-5（Cyclin-DependentKinase-5，CDK5)为多功能丝氨酸/苏氨酸蛋白激酶家族中的一个重要成员。它不参与细胞周期调控，但在神经系统的发育、神经退行性疾病、癌症的病理进程等中扮演了重要的角色。CDK5可以特异性地磷酸化包含有(S/T)PX(H/K/R)序列的底物蛋白，其中S/T分别表示丝氨酸/苏氨酸，X代表任意氨基酸，P为脯氨酸。 AKT也同为丝/苏氨酸激酶，参与胰岛素信号通路的大部分代谢活动，并在细胞的增殖、凋亡、分化、有丝分裂、细胞的迁移、细胞骨架重排等多种生理变化过程中充当关键角色。在与PI3K组成的PI3K/AKT重要信号通路中，激活的PI3...Cyclin-dependent protein kinase-5 (CDK5) is an important member of serine / threonine protein kinase family.It is not involved in the cell cycle regulation, but plays an important role in the development of the nervous system and neurodegenerative diseases, cancer pathology process. CDK5 can specificially phosphorylate its substrates which contain (S/T) PX(H/K/R) sequences, wherein S/T represent S...学位：理学硕士院系专业：医学院_生理学学号：2452013115342

Determination of Fat soluble Components in Danshen by High-ResolutionElectrospray Ionization Time-of-flight Mass Spectrometry

An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home built HR ESI TOF MS was applied to detect fat soluble components in Danshen( Salvia miltiorrhiza Bage ) directly. Compared with the method of HPLC ESI MS(High Performance Liquid Chromatograph Electrospray ionization Mass Spectrometry) method, HR ESI TOF MS method is more accurate, quick, simple and reliable, so the method can become an effective tool for the quality control of TCM.国家自然科学专项基金 (批准号 :H -2 992 70 0 2 );; 国家自然科学重点基金 (批准号 :2 92 45 110 );; 香港特区政府创新科技 署;; 科技部中药现代化重中之重“九五”项目资

Multi-level structural mechanical behavior and surface contact characteristics of fabric-reinforced rubber seals

As a critical component of aircraft, fabric-reinforced rubber seals primarily are designed to provide reliable sealing performance. The distribution of surface contact stress directly determines the sealing effectiveness and has a significant impact on the design of aircraft door. This study systematically investigates the macroscopic mechanical behavior and surface contact stress distribution characteristics of the Auxiliary power unit door's fabric-reinforced rubber seals. First, based on the representative volume element model and finite element analysis, the mechanical properties and overall macrostructure of the microscopic components of the seal were characterized, and the accuracy and reliability of the finite element model were verified by experiments. Subsequently, the effects of varying compression levels on surface contact characteristics were analyzed, revealing the evolution and distribution patterns of surface contact stress on the seals. Finally, the influence of different bending degrees and widths on the stress distribution and surface contact characteristics of the seals was examined. This research develops a framework for the multi-level structural mechanical behavior of fabric-reinforced rubber. The results provide a quantitative analysis method and design basis for optimizing the compatibility between seals and aircraft door frame, which enhances the sealing performance and reliability of the structure