Genomic Library Construction, Random Sequencing, and Gap and SufC Function of Halomonas meridiana

Halomonasmeridiana（盐单胞菌属）是James等（1990）首次从南极盐湖（盐度从4‰～174‰）中分离出的一株耐盐的盐单胞菌属新种。此属的细菌多生活于极端环境中，既往有关此属细菌的工作仅限于分类和一些极端酶的开发方面，但在基因组学或蛋白质组学方面的研究尚未见报道。本实验首先运用蛋白质组学技术---2-D和质谱技术对H.meridiana的外膜蛋白进行相关研究。由于在数据库中缺少与H.meridiana同属或亲缘关系相近种属的基因组测序结果，限制了依赖于大型数据库的质谱技术的充分运用，为鉴定工作带来困难，难以获得完全可靠的蛋白信息。于是，我们采用了类似真核生物基因组研究的EST...Halomonas meridiana, surviving in extreme environment, was a novel specie first isolated by James etc from the salt lake in Antarctic in 1990. Information regarding to classification and development of extreme enzymes is available now, yet few studies are involved in the genomics and proteomics for this bacteria. Firstly, the proteomics methodologies were applied to characterize the outer...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：20032611

新型冠状病毒肺炎患者的心理特点及疏导

目的：研究新型冠状病毒肺炎患者的心理特点及心理疏导方法。方法：选择 2020 年 1 月 -2020 年 4 月本医院收治的 60 例新型冠状病毒肺炎患者，“随机数字表法”分观察组 ( 根据心理特点、提供心理疏导 ) 与对照组 ( 常规护理 ) 各 30 例，两组护理效果比较。结果：护理前比较两组心理情绪无统计学差异，P ＞ 0.05；护理后与对照组比较，观察组 SAS、SDS 值较低；观察组满意率 (93.33%) 高于对照组 (73.33%)，P ＜ 0.05。结论：根据新型冠状病毒肺炎患者的心理特点采用心理疏导护理能改善心理应激反应、提高配合度，值得借鉴。　</jats:p

The Genomic Library Construction and Clone Sequence BLAST of Deep-sea Bacterium WP2

以pUC19质粒DNA作为载体,用BamH1酶切、碱性磷酸酶处理后,分别与Sau3AⅠ部分酶切的Shewanellasp.(WP2)基因组DNA 2-6kb的DNA片段连接,电击转化大肠杆菌DH5α感受态,在含有Amp的LB平板上筛选重组子,得到转化子约5~6×103;随机挑取100个克隆子,经DNA测序,通过在网络数据库中进行BLASTX分析,得到一系列相对保守蛋白的比对结果.这些结果为遗传背景不清楚的细菌的研究,提供了一条从分子水平上探讨的技术路线.A genomic library of a deep-sea bacterium was constructed by using pUC19 plasmid as vector.PUC19 was digested by BamH1 enzyme,treated with CIP,and then ligated with 2-6 Kb fragments of Shewanella sp.(WP2)digested by Sau3A 1.These ligation mixtures were electransformed to E.coli DH5 α.The recombinants were screened on the LB plate with Amp and 5~6×103 recombinants were obtained.Then 100 positive clones were randomly selected and then sequenced.The sequences were searched in NCBI using BLASTX tool and the homology of 49 sequences were obtained for further study.Our results suggest that our strategy may be an approach for study of a bacterium without genetic knowledge at molecular level.国际海底区域研究开发“十五”课

基于高分2号遥感数据估测中亚热带天然林木本植物物种多样性

【目的】探索高分2号遥感数据与中亚热带天然林木本植物物种Shannon-Wiener多样性指数、Simpson多样性指数和Pielou均匀性指数之间的关系,为森林经营管理和保护策略提供参考。【方法】提取高分2号多光谱数据的原始波段、植被指数、纹理特征和全色波段纹理特征,使用随机森林算法筛选变量并对3种多样性指数进行建模,设置不同纹理提取窗口来寻找最优窗口。【结果】基于随机森林算法的RFE冗余变量去除方法可从众多遥感变量中快速选择对模型精度具有显著贡献的少量变量。多光谱数据3×3窗口纹理特征、全色数据7×7窗口纹理特征和植被指数结合的特征集对3种多样性指数具有较好估测结果,其决定系数(R~2)和均方根误差(RMSE)分别为0.47和0.300(Shannon-Wiener多样性指数)、0.53和0.042 (Simpson多样性指数)、0.61和0.051 (Pielou均匀性指数)。植被指数中类胡萝卜素反射率指数与3种多样性指数具有显著相关关系。【结论】高分2号遥感数据中的植被指数和纹理特征可有效估测研究区森林木本植物物种多样性。类胡萝卜素反射率指数可体现森林中类胡萝卜素相对于叶绿素的含量,在秋冬季节作为反映常绿树种和落叶树种分布的指数,对森林木本植物物种多样性估测具有最大贡献。使用星载遥感数据预测的多样性和均匀性指数分布可有效监测森林木本植物物种多样性变化

Purification,Crystallization and Crystallographic Analysis of the Pelota C-terminal Domain from Human

Pelota在进化上是非常保守的RNA结合蛋白,人源Pelota; mRNA分布于几乎所有的组织并作为一个多功能的蛋白参与多种生物途径.为解析人源Pelota C端结构域(C-terminal; domain,CTD)的晶体结构,首先在大肠杆菌(Escherichia; coli)中表达,并采用亲和层析、凝胶过滤柱层析的方法,获得了纯度大于97%的蛋白.动态光散射实验表明纯化的蛋白有较高的均一性.在筛选了1; 852个结晶条件后,优化的人源Pelota CTD蛋白晶体能衍射X射线至0.26; nm分辨率.蛋白晶体的空间群为${\rm{P}}{{\rm{6}}_{\rm{5}}}{\rm{22}}$,晶胞常数$a{\rm{ -; 7}}{\rm{.882nm,}}b{\rm{ = 7}}{\rm{.882nm,}}c{\rm{ =; 19}}{\rm{.746nm}}$.上述结果为进一步研究Pelota的功能及其与下游蛋白的相互作用奠定了结构基础.Pelota,an evolutionarily conservative RNA binding protein,is distributed; in almost all tissues and involved in a variety of cell biological; regulation as a multifunctional protein.In order to determine the; crystal structure of the human Pelota C domain (C-terminal; domain,CTD),we chose Escherichia coli to express the protien and; purified the protien by affinity chromatography,gel filtration; chromatography.Finally,the protein is over 97% in purity.Dynamic light; scattering experiments showed that the purified protein had high; homogeneity.After screening the 1 852 crystallization conditions,the; optimized crystal of the human Pelota C domain can be diffracted to 0.26; nm resolution.The space group of the crystal is; ${\rm{P}}{{\rm{6}}_{\rm{5}}}{\rm{22}}$,and the unit cell constant is; $a{\rm{ - 7}}{\rm{.882nm,}}b{\rm{ = 7}}{\rm{.882nm,}}c{\rm{ =; 19}}{\rm{.746nm}}$.We determine the crystal structure of human Pelota; CTD in this study,which lays a foundation for further study on the; function of Pelota protein and its interaction with the downstream; proteins.高等学校学科创新引智计划("111"计划

大连城市居住适宜性的空间评价

为创造更好、更适宜人们居住、生活和工作的空间,不断提高市民的生活质量,选择城市的生活方便性、健康性、居住安全性、环境舒适度和出行方便性等为指标,在问卷调查量化数据的基础上,采用层次分析法和Q型聚类分析方法,从行政区和不同功能片区两个角度,进行了大连城市居住适宜性空间评价,以期为大连城市建设提供参考。研究表明:大连城市居住适宜性以位于市区政治经济商业中心的区域得分最高,西、北部市郊结合部排名最后,部分远郊区宜居性虽然较好,但各指标分值差异却很大。总体而言,大连城市宜居性在空间格局上体现出由中心城区向外围逐渐降低、差异程度变大的特性,该空间特征分析结论与行政区评价结果具有很好的一致性

Xylanase Production on Bagasse with Solid State Fermentation by Aspergillus clavatus UA 2

棒曲霉（ASPErgIluSClAVATuS）uA-2固态发酵蔗渣产木聚酶的培养基为每克蔗渣加营养液5Ml，初始PH8.5.营养液的适宜组成为每升含：nH4nO310g、k2HPO46g、MgSO4·7H2O0.75g、CACl20.75g、fESO4·7H2O11.25Mg、MnSO4·H2O3.75Mg、znSO43.0Mg、COCl24.5Mg.在上述培养基中接入其湿重10%（W/W）的新鲜的uA-2种子曲，28℃培养108H，其木聚糖酶，滤纸降解酶和羧甲基纤维素酶的活力分别为2695.6、28.5和42.7u/g蔗渣.酶反应的最适PH5.0，最适温度50℃；在PH4.0~11.0内酶活性十分稳定.50℃保温1H，酶活剩余55%，8℃下放置23d，活力几乎不变.磷酸盐，半胱氨酸对酶有激活作用，EdTA和对氯汞本甲酸对酶有抑制作用The medium composition For the xylanase production on bagasse with solid state Fermentation by Asp.clavatus UA 2 and the characteristics of crude enzyme preparation were examined.Composition of the suitabe medium was as Follows: 5 ml of nuttrient solution were added For one gram bagasse with initial pH 8.5.The suitable nutrient solution consisted of ∶10 g NH 4HO 3, 6 g K 2HPO 4,0.75 g MgSO 4·7H 2 O,0.75 g CaCl 2,11.25 mg FeSO 4·7H 2 O,3.75 mg MnSO 4·H 2O,3.0 mg ZnSO 4,4.5 mg CoCl 2 and 1 000 ml of distilled water.The amount of seed cultures inoculated was 10 % of the medium moisture weight.AFter growing at 28 ℃ For 108 h, the activity of xylanase, Filter paper degradase and carboxymethylcellulase were 2 695.6 u/g,28.5 u/g and 42.7 u/g,respectively.The optimal pH and temperature For xylanase reaction were pH 5.0 and 50 ℃, respectively, Having incubated the enzyme at 50 ℃ For 1 h, the enzyme retatined 55 % of its original activity.The xylanase activity was hardly changed upon storage at 8 ℃ For 23 days.EDTA and p chloromerouribenzoic acid inhibited xylanase activity, but phosphate and cysteine increased its activity