Simultaneous Laser-Induced Fluorescence And Contactless-Conductivity Detection For Microfluidic Chip

A combined detection system involving simultaneous LIF and contactless-conductometric measurements at the same place of the microfluidic chip was described. The LIF measurement was designed according to the confocal principle and a moveable contactless-conductivity detector was used in (CD)-D-4. Both measurements were mutually independent and advantageous in analyses of mixtures. Various experimental parameters affecting the response were examined and optimized. The performances were demonstrated by simultaneous detection of Rhodamine B. And the results showed that the combined detection system could be used sensitively and reliably. (C) 2008 Yong Yu. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved

静电相互作用对纤维蛋白质原在金表面吸附的影响及牛血清蛋白质与胶原蛋白质竞争吸附的AFM研究

固体表面的性质对蛋白质的吸附具有很大的影响作用,蛋白质在固体表面的吸附可分为共价吸附和非共价吸附.非共价吸附主要包括氢键、疏水力和静电力三种相互作用.其中静电相互作用对蛋白质吸附的影响仍未有一致的看法.该文将椭偏显微成像系统与电化学方法联用来研究静电相互作用在蛋白质吸附中的影响.在0.1MKHPO-NaHPO(pH=7.80)溶液中,金膜表面零电荷电势(PZC)在-0.60V(vs.SCE)左右.开路电位为+0.10V(vs.SCE),大于零电荷电势(PZC),说明金膜表面带正电荷.假设蛋白质在金表面表面吸附后,没有蛋白质吸附区域的单位面积的电容与吸附前时的单位面积的电容相同.计算出在FIB吸附达到饱和后实际蛋白质真正占据的比表面仅为36.7%

The Multiple Objective Decision And Resolution of Goal Models For Financial Planning

本文用规划论和系统论的原理和方法，建立了福建省龙岩市财政发展的多目标决策模型和财政支出的目标分解模型。通过对模型的求解及其结果分析，给出了龙岩市财政最优发展规划，为该市的领导提供决策依据。Using the principles and methods of Programming Theory and System Theory, this paper has formed the Multiple Objective Decision Model for financial planning, and the Resolution of Goal Model for revenue and expenditure of longyan City. Solving these models and analyzing the results, the author has come out with the optimal programming for financial planning of longyan City, and provided the basis ...学位：理学硕士院系专业：数学系_概率论与数理统计学号：1991290

Study of the Adsorption of Fibrinogen on Gold-Coated Silicon Wafer by an Impedance Method

In 0.1 mol/l KH_2PO_4-Na_2HPO_4 (pH 7.80) buffer solution, the potential of zero charge (PZC) and the open circuit potential of gold-coated silicon were determined to be about -0.6 and +0.10 V (vs SCE), respectively. The open circuit potential was higherIn 0.1 mol/l KH_2PO_4-Na_2HPO_4 (pH 7.80) buffer solution, the potential of zero charge (PZC) and the open circuit potential of gold-coated silicon were determined to be about -0.6 and +0.10 V (vs SCE), respectively. The open circuit potential was highe

溶菌酶分子聚集状态对蛋白质晶体生长

使用动态光散射仪及微批量法袁实时测量了溶菌酶晶体生长过程中袁蛋白质颗粒的聚集 情况遥实验表明当液滴中的溶菌酶分子单体形式占多数袁同时也存在一定数目的聚集体时袁将 会产生晶体遥另外还通过观察有无絮状物附着情况下袁溶菌酶晶体的生长情况袁研究了絮状物 对晶体生长速度的影响遥实验表明这种由高密度的蛋白质聚集体组成的絮状物附着在晶体表面 时袁晶体的生长受到抑制遥而絮状物会逐渐解体袁重构成四方晶体或球状结晶等更稳定的聚集 状态遥研究在一定程度上揭示了溶液中溶菌酶分子的聚集状态与结晶的关系