5 research outputs found

    Белорусско-украинское сотрудничество в обустройстве совместной границы.

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    В статье рассматривается создание и развитие пунктов пропуска на белорусско-украинской границе. Подчеркивается, что развитая пограничная инфраструктура являлась залогом активизации межгосударственных, приграничных контактов, а также транзитного движения. Несмотря на трудности протекания межгосударственного договорно–правового оформления государственной границы, сопредельные страны смогли решить и воплотить в жизнь ряд вопросов, регулирующих режим и правила пересечения границы, организацию пассажиро- и грузоперевозок, а также обеспечить правопорядок и законность на общем участке границы. Проанализированы особенности 94 расположения погранпереходов на территории приграничных с Украиной областей Беларуси. Определены основные направления взаимодействия пограничных и таможенных служб сопредельных стран по обеспечению полноценного функционирования общего участка границы. = This article is devoted to creation and development of customs points on Belarusian – Ukrainian border. The author emphasizes that developed border infrastructure led to activization of interstate contacts and transit traffic. In spite of difficulties connected with making border contracts, neighbouring countries found solutions for many problems. As a result the regime and rules of crossing border were established; transportation of passengers and cargoes was organized and in addition order and legality on the border were provided. The author analyses peculiarities of the location of border crossing points on the Belarusian – Ukrainian border. In the article basic directions of the cooperation of border and customs services are determined; the main purpose of them is providing continuous function of border posts

    The first post - war elections to the supreme soviet of the BSSR in 1947. Course and organisation. On the example of the Maloritsky district of the Brest Region

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    This article is devoted to the conduct of the first post-war elections to the Supreme Soviet of the BSSR - methods of agitation of the population, the organization of the election process, as well as the difficult political situation in the region of western Belarus in the first post-war years. The election process in the Maloritskiy district of the Brest region is chosen as an example.Данная статья посвящена проведению первых послевоенных выборов в Верховный Совет БССР – методам агитации населения, организации процесса выборов, а также непростой политической обстановке в регионе западной Беларуси в первые послевоенные годы. В качестве примера выбран процесс выборов в Малоритском районе Брестской области

    Belarusian–ukrainian cooperation in arrangement of joint border

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    В статье рассматривается создание и развитие пунктов пропуска на белорусско-украинской границе. Подчеркивается, что развитая пограничная инфраструктура являлась залогом активизации межгосударственных, приграничных контактов, а также транзитного движения. Несмотря на трудности протекания межгосударственного договорно–правового оформления государственной границы, сопредельные страны смогли решить и воплотить в жизнь ряд вопросов, регулирующих режим и правила пересечения границы, организацию пассажиро- и грузоперевозок, а также обеспечить правопорядок и законность на общем участке границы. Проанализированы особенности 94 расположения погранпереходов на территории приграничных с Украиной областей Беларуси. Определены основные направления взаимодействия пограничных и таможенных служб сопредельных стран по обеспечению полноценного функционирования общего участка границы.This article is devoted to creation and development of customs points on Belarusian– Ukrainian border. The author emphasizes that developed border infrastructure led to activization of interstate contacts and transit traffic. In spite of difficulties connected with making border contracts, neighbouring countries found solutions for many problems. As a result the regime and rules of crossing border were established; transportation of passengers and cargoes was organized and in addition order and legality on the border were provided. The author analyses peculiarities of the location of border crossing points on the Belarusian– Ukrainian border. In the article basic directions of the cooperation of border and customs services are determined; the main purpose of them is providing continuous function of border posts

    PCR with Paracoccidioides brasiliensis specific primers: potential use in ecological studies PCR com «primers» específicos de Paracoccidioides brasiliensis: uso potencial em estudos ecológicos

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    The precise microenvironment of Paracoccidioides brasiliensis has not yet been discovered perhaps because the methods used are not sensitive enough. We applied to this purpose the polymerase chain reaction (PCR) using three sets of specific primers corresponding to two P. brasiliensis genes. This fungus as well as several other fungi, were grown and their DNA obtained by mechanical disruption and a phenol chloroform isoamylalcohol-based purification method. The DNA served for a PCR reaction that employed specific primers from two P. brasiliensis genes that codify for antigenic proteins, namely, the 27 kDa and the 43 kDa. The lowest detection range for the 27 kDa gene was 3 pg. The amplification for both genes was positive only with DNA from P. brasiliensis; additionally, the mRNA for the 27 kDa gene was present only in P. brasiliensis, as indicated by the Northern analysis. The standardization of PCR technology permitted the amplification of P. brasiliensis DNA in artificially contaminated soils and in tissues of armadillos naturally infected with the fungus. These results indicate that PCR technology could play an important role in the search for P. brasiliensis’ habitat and could also be used in other ecological studies.<br>O microambiente adequado do Paracoccidioides brasiliensis não foi ainda bem esclarecido, talvez porque os métodos utilizados não sejam suficientemente sensíveis. Aplicamos com este propósito, a reação em cadeia da polimerase (PCR) usando três jogos de primers específicos do P. brasiliensis, correspondendo a dois dos genes do P. brasiliensis. Este fungo, assim como outros fungos, foram cultivados e seus DNAs obtidos por ruptura mecânica e purificados com mistura de fenol-clorofórmio com álcool isoamílico. Os DNAs serviram para a reação de PCR utilizando-se primers específicos para dois dos genes do P. brasiliensis que codificam para as proteínas antigênicas, denominadas, 27 kDa e 43 kDa. O limite mínimo de detecção para o gene 27 kDa foi de 3 pg. A amplificação para os dois genes foi positiva só com o DNA do P. brasiliensis; além disso, o mRNA para o gene de 27kDa estava presente apenas no material do P. brasiliensis, como mostrado pela análise por Northern-blot. A padronização da técnica do PCR permitiu a amplificação do DNA do P. brasiliensis em solos contaminados artificialmente com o fungo e em tecidos de tatus infectados na natureza. Estes resultados indicam que a técnica do PCR podería ter um papel muito importante na pesquisa do habitat do P. brasiliensis e, além disso, podería ser utilizada em outros estudos ecológicos
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