14 research outputs found
Menstrual cycle variability of ca 72-4 in healthy women
Objectives: CA 72-4 is not approved as a tumormarker but has been used as an adjunct marker in gynecological
practice. The study aims to evaluate the menstrual cycle variability of CA 72-4 in a population of healthy
women.
Design and methods: Forty apparently healthy regularly menstruating subjects were included in the crosssectional
study designed in the University Obstetrics and Gynecology outpatient clinic. Venous blood samples
from each participant were collected twice: first at the follicular phase (2nd–5th days of the menstrual cycle)
for FSH, estradiol, CA 125, CA 72-4 and the other at the luteal phase (21st-24th days of the menstrual cycle)
for progesterone, CA 125 and CA 72-4 levels.
Results: CA 72-4 values were similar in follicular and luteal phase of the menstrual cycle in apparently
healthy regularly menstruating subjects (1.15 U/mL (0.2–5.4) vs 1.15 U/mL (0.56–6.3); p= 0.326 respectively).
Ovulatory or smoking status did not have an effect on CA 72-4 values (p N 0.05).
Conclusion: This first clinical study about the menstrual cycle variability of CA 72-4 revealed that the menstrual
cycle does not have a significant impact on CA 72-4 values and that it can be measured at any time during
the menstrual period
PPI kullanımının clostridium difficile serum antijen değerlerine olan etkisi
Aim: To evaluate the effects of proton-pump inhibitor (PPI) use on distrubtion of intestinal flora by measuring serum Clostridium Difficile antigen levels before and at the end of a three- months treatment in patients with Laryngopharyngeal reflux (LFR) treatment. Material and Method: The study covers 32 patients with LFR, out of which 24 were female (75%) and 8 were male (25%). The mean age of the patients was 34.13 +/- 11.59. All patients included in the study were administered Lansoprazole 30 mg tablets perorally before meals and twice a day for treatment. Reflux Symptom Index (RSI), Reflux Finding Score (RFS), white blood cell count, CRP and serum Clostridium Difficile toxin A, B measurement results were comparatively evaluated through the blood serum samples drawn from the patients before and at the end of the three- months treatment. Results: While the mean values of pre-treatment RSI and RFI were 20.81 +/- 4.05 and 13.31 +/- 3.30 respectively, the mean values were measured to be 3.41 +/- 2.37 and 1.50 +/- 1.88 respectively following the three- months treatment (p< 0.05). The pre-treatment mean value of serum Clostridium Difficile Ag was 140.56 +/- 11.74, while it was seen that the same value became 114.94 +/- 10.70 after the three- months treatment (p< 0.05). There was, however, no statistically significant change in the other parameters. Discussion: According to the results obtained, it was seen that the treatment with PPI was not cause to increase Clostridium difficile toxin A, B serum antigen levels. So these drugs could be used in long time therapies confidently.Amaç: Larengofarengeal Reflü (LFR) hastalığının tedavisi amacıyla Proton pompa inhibitörü (PPİ) kullanan hastalarda tedavi öncesi ve üç aylık tedavinin sonunda serum Clostridium Difficile antijen miktarı ölçülerek PPİ kullanımının
serum Clostridium Difficile serum antijen miktarı üzerindeki etkisinin değerlendirilmesi. Gereç ve Yöntem: Çalışmaya LFR hatalığı tespit edilen 32 hasta ( 24 ( % 80)’ü kadın, 8 (% 20)’i erkek) dahil edildi. Hastaların yaş ortalaması 34.13 ± 11.59 idi. Çalışmaya dahil edilen tüm hastalara tedavi için günde iki kez yemeklerden önce alınacak şekilde lansoprazol 30 mg tablet peroral olarak başlandı. Tedavi öncesinde ve üç aylık tedavinin sonunda hastaların LFR şiddeti Reflü Semptom İndeksi ( RSI) ve RBS ( Reflü Bulgu Skoru) kullanılarak değerlendirildi. Ayrıca hastalardan tedavi öncesi ve üç aylık tedavinin sonunda alınan kan serum örneklerinde beyaz küre, CRP, sedimantasyon
ve serum Clostridium Difficile toxin A, B ölçümleri yapılarak karşılaştırmalı olarak değerlendirildi. Bulgular: Tedavi öncesi RSI ve RBS ortalamaları sırasıyla 20.81±4.05 ve 13.31±3.30 iken, üç aylık tedavi sonrasında bu ortalamalar sırasıyla 3.41±2.37 ve 1.50±1.88 olarak tespit edildi ( p< 0,05). Tedavi öncesi serum Clostridium Difficilie Ag değerlerinin ortalaması 140.56±11.74
iken üç aylık tedavi sonrasında bu ortalamanın 114.56±10.70 olduğu görüldü ( p 0,05). Tartışma: Elde ettiğimiz sonuçlara göre, proton pompa inhibitörü kullanan hastaların Clostridium difficilie toxin A, B serum antijen düzeylerinde istatistiksel olarak anlamlı bir düşüş olduğunu görüldü
Anti-Mullerian hormone and insulin resistance in classic phenotype lean PCOS
Purpose This study is designed to explore the correlation
between AMH levels and IR in normal weight PCOS
women.
Materials and methods This prospective study was conducted
on 55 patients, who were admitted to obstetrics and
gynecology department of a university clinic. Study group
was consisted of 34 patients diagnosed as polycystic ovary
syndrome (PCOS) according to the Rotterdam Criteria,
whereas control group was consisted of 21 healthy volunteers
without any features of clinical or biochemical hyperandrogenism,
who had regular menstrual cycles. BMI C 25 kg/m2
were considered overweight and obese and excluded. Blood
samples were obtained during days 2–3 after spontaneous
menses or progesterone-induced withdrawal bleeding after
overnight fasting for at least 12 h. The weight, height, hip and
waist circumferences of the patients were measured. Fasting
insulin and glucose (FPG) levels were used for calculating
different insulin resistance indexes (Homeostatic Model
Assessment (HOMA-IR), Quantitative Insulin Sensitivity
Check Index (QUICKI)).
Results No significant difference was found between
PCOS and control groups regarding the mean age, BMI,
waist to hip ratio (WHR), mean values of FPG, FPG/insulin
ratio and HOMA B (p[0.05). AMH values were significantly
higher in PCOS cases when compared with controls
(4.7 vs. 3.4 ng/mL) (p\0.05).The mean values of HOMAIR
and QUICKI indexes were significantly higher among
PCOS cases when compared with controls. E2 levels were
significantly lower and Total-T were significantly higher in
PCOS patients. When PCOS cases are categorized according
to the existence of IR, no difference in Total-T and AMH
levels between both groups. Although not statistically significant,
a negative correlation of AMH with HOMA-IR and
a positive correlation with QUICKI index were found.
Among the hormone parameters, AMH was found to be
positively correlated with Total-T (r = 0.332, p = 0.013).
Conclusion Although the relation between AMH and
androgen production is supported by current evidence, the
mechanism underlying the relation between AMH and
insulin resistance is not clear yet
The impact of route of anesthesia on maternal and fetal ischemia modified albumin levels at cesarean section: a prospective randomized study
Objective: Ischemia modified albumin has been shown to
increase in ischemic situations, and has also been shown
to increase in fetal cord blood in deliveries by cesarean
section. The aim of this study is to reveal whether anesthesia
has an impact on maternal and fetal cord ischemia
modified albumin levels.
Methods: Seventy two women with uncomplicated term
pregnancies were randomized to spinal (n = 37) or general
anesthesia (n = 35) groups. The blood pressure, oxygen
saturation, and pulse rate of the patients were recorded
during the procedure. Maternal blood samples of ischemia
modified albumin (IMA) were taken 10 min from the start
of the procedure. The fetal cord blood samples of IMA
were taken immediately after birth.
Results: Maternal (0.99 ± 0.19 vs. 0.80 ± 0.27) and fetal
(1.00 ± 0.21 vs. 0.70 ± 0.26) IMA levels were significantly
higher in the general anesthesia group. Fetal IMA levels
were positively correlated with maternal gravidity (r = 0.31;
P = 0.008), parity (r = 0.25; P = 0.028), and fetal birth weight
(r = 0.23, P = 0.045). Also, as time from incision to delivery
lengthens, fetal IMA levels increase (r = 0.29, P = 0.012).
Conclusion: Fetal cord ischemia modified albumin levels
were higher in the general anesthesia group, therefore, it
is proposed that regional anesthesia should be the preferred
route of anesthesia for an elective cesarean section,
at least until the impact of high fetal cord IMA levels are
manifested
Atherogenic dyslipidemia, subclinical atherosclerosis, non-alcoholic fatty liver disease and insulin resistance in polycystic ovarian syndrome
Objective: We aimed to explore the relationship between insulin resistance (IR) and small
dense lipoprotein (sd-LDL) particles, carotid intima-media thickness (CIMT) and non-alcoholic
fatty liver disease (NAFLD) in young normal weight PCOS cases.
Methods: This prospective, case-control study was designed in a University Hospital and 34
women with PCOS and 21 healthy controls were enrolled. Fasting plasma glucose, insulin,
lipid (including sd-LDL particles) and hormone profiles, abdominal ultrasound and CIMT were
evaluated.
Results: IR was present in 68% of PCOS group while in none of controls. High density
lipoprotein (HDL), very low density lipoprotein (VLDL), triglycerides (TG), and sd-LDL
were higher in patients with IR (p<0.05). A positive correlation of sd-LDL with IR, VLDL and
TG was found. A significantly higher rate of NAFLD and CIMT was found in PCOS. Totaltestosterone
levels were weakly and positively correlated with CIMT (r=0.277, p=0.041).
Conclusion: Insulin resistance and NAFLD are highly prevalent among young normal weight
PCOS patients. When compared to controls levels of sd-LDL and CIMT are increased in PCOS.
Insulin resistance is the key parameter for NAFLD and atherogenic dyslipidemia in PCOS.
Hence, screening for NAFLD may be valuable for detection and prevention of liver disease.
Higher levels of sd-LDL in insülin resistant PCOS cases necessiates treating PCOS for I
Maternal and umbilical cord ischemia-modified albumin levels in nonreassuring fetal heart rate tracings regarding the mode of delivery
Objective: To evaluate umbilical cord blood ischemia-modified
albumin (IMA) levels in cases of fetal distress (FD) and to explore
fetal blood IMA levels regarding the route of delivery. Methods:
Umbilical cord and maternal serum IMA concentrations were
assessed in term 40 cases with cesarean section (CS) due to FD,
76 cases with elective repeat CS and 85 cases with noncomplicated
vaginal delivery. Results: The maternal and umbilical cord
IMA levels were significantly lower in vaginal deliveries when
compared with CS cases either in FD or previous CS groups
(p = 0.02). Although no statistically significant difference was
found in IMA levels of CS groups (previous CS vs. FD), cord blood
IMA levels tend to be higher in FD group. Neither demographic
characteristics nor fetal outcome parameters were found to have
any correlation with maternal IMA levels. However, umbilical
cord IMA levels were found to be negatively correlated with 1th
min Apgar scores (r = –0.143, p = 0.043). Conclusions: IMA seems
to be responsive to hypoxic FD showing the highest levels in
cases with severe fetal hypoxia. Higher levels of IMA in cases
with elective repeat CS might indicate acute transient hypoxia
and possible myocardial ischemia in these cases
Gebelik döneminde hba1c ve fruktozaminin glisemik kontrol belirteci olarak önemi ve bazı biyokimyasal paramatrelerin değerlendirmesi
[No Abstract Available
Quantification of Anandamide and 2-Arachidonylglycerol in Plasma Samples: A Short, Non-toxic HPLC Method and Sample Storage
Objective: The endocannabinoid system plays an important modulatory role in brain physiology, pain sensation, appetite regulation, cardiovascular system, female reproductive system and the immune system. The endogenous ligands of cannabinoid receptors, anandamide (ANA) and 2-Arachidonylglycerol (2-AG) have been identified in various mammalian tissues. However, it difficult to quantify them accurately in blood and in tissues since these endogenous cannabinoids are found in small amount and they are metabolized very quickly. In order to quantify ANA and 2-AG from blood accurately, it was aimed (a) to determine pre-analytical conditions in blood sampling procedure, (b) to improve extraction process and quantification of ANA and 2-AG in plasma samples by HPLC, and (c) to determine storage conditions and period of these metabolites