32 research outputs found
Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour
<p>Abstract</p> <p>Background</p> <p><it>Phytophthora infestans </it>(Mont.) de Bary, the causal organism of late blight, is economically the most important pathogen of potato and resistance against it has been one of the primary goals of potato breeding. Some potentially durable, broad-spectrum resistance genes against this disease have been described recently. However, to obtain durable resistance in potato cultivars more genes are needed to be identified to realize strategies such as gene pyramiding or use of genotype mixtures based on diverse genes.</p> <p>Results</p> <p>A major resistance gene, <it>Rpi-rzc1</it>, against <it>P. infestans </it>originating from <it>Solanum ruiz-ceballosii </it>was mapped to potato chromosome X using Diversity Array Technology (DArT) and sequence-specific PCR markers. The gene provided high level of resistance in both detached leaflet and tuber slice tests. It was linked, at a distance of 3.4 cM, to violet flower colour most likely controlled by the previously described <it>F </it>locus. The marker-trait association with the closest marker, violet flower colour, explained 87.1% and 85.7% of variance, respectively, for mean detached leaflet and tuber slice resistance. A genetic linkage map that consisted of 1,603 DArT markers and 48 reference sequence-specific PCR markers of known chromosomal localization with a total map length of 1204.8 cM was constructed.</p> <p>Conclusions</p> <p>The <it>Rpi-rzc1 </it>gene described here can be used for breeding potatoes resistant to <it>P. infestans </it>and the breeding process can be expedited using the molecular markers and the phenotypic marker, violet flower colour, identified in this study. Knowledge of the chromosomal localization of <it>Rpi-rzc1 </it>can be useful for design of gene pyramids. The genetic linkage map constructed in this study contained 1,149 newly mapped DArT markers and will be a valuable resource for future mapping projects using this technology in the <it>Solanum </it>genus.</p
Diversity analysis of Rpi-ber1 and Rpi-vnt1 genes determining broad spectrum resistance to Phytophthora infestans
Poster presented at EAPR Pathology & Pests Section Meeting, Arras, France
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
Presence of homologues of the PVY resistance gene Rysto in wild relatives of potato
Poster presented at 12TH INTERNATIONAL CONGRESS OF PLANT PATHOLOGY ICPP2023, Lyon, France
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
PacBio amplicon sequencing of Rysto homologues in wild potato species
Poster presented at EAPR Pathology & Pests Section Meeting, Arras, France
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
Amplicon sequencing of NBS LRR genes conferring resistance to Phytophthora infestans in potato
Poster presented at 2023 IS-MPMI Congress, Molecular Plant-Microbe Interactions, Providence, USA
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
High throughput analysis of Rpi genes in potato cultivars, breeding lines and wild Solanum species
Poster presented at 12TH INTERNATIONAL CONGRESS OF PLANT PATHOLOGY ICPP2023, Lyon, France
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
Effector gene variation in Polish and Norwegian Phytophthora infestans strains
Poster presented at 2023 IS-MPMI Congress, Molecular Plant-Microbe Interactions, Providence, USA
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
Aggressiveness test of Phytophthora infestans isolates with different effector alleles
Poster presented at EAPR Pathology & Pests Section Meeting, Arras, France
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055
Differences in Avr-vnt1 alleles and aggressiveness in four European Phytophthora infestans lineages
Poster presented at 12TH INTERNATIONAL CONGRESS OF PLANT PATHOLOGY ICPP2023, Lyon, France
The research leading to these results has received funding from the Norwegian Financial Mechanism 2014-2021, project DivGene: UMO2019/34/H/NZ9/0055