4 research outputs found
Ultrahigh-Performance Liquid ChromatographyâHigh-Resolution Quadrupole Time-of-Flight Mass Spectrometry Based Metabolomics Reveals Key Differences between Brachiaria decumbens and B. brizantha, Two Similar Pastures with Different Toxicities
Several
species of Brachiaria (Poaceae)
currently cover extensive grazing areas in Brazil, providing valuable
source of feed for a large cattle population. However, numerous cases
of toxicity outbreaks in livestock have raised concerns on safety
of using these plants, especially B. decumbens. In this study, chemometric analysis of ultrahigh-performance liquid
chromatographyâhigh-resolution quadrupole time-of-flight mass
spectrometry (UHPLCâHR-QTOF-MS) data has for the first time
uncovered qualitative and quantitative differences between metabolomes
of toxic B. decumbens and nontoxic B. brizantha. The steroidal saponin protoneodioscin
was established as the main biomarker for B. decumbens when compared to B. brizantha, and
therefore the key explanation for their phytochemical differentiation.
Quantification of protodioscin in both plants showed no significant
differences; consequently, the idea that this compound is solely responsible
for toxicity outbreaks must be discarded. Instead, we propose that
the added occurrence of its stereoisomer, protoneodioscin, in B. decumbens, can be considered as the probable cause
of these events. Interestingly, the greatest concentrations of saponins
for both species were reached during winter (B. decumbens = 53.6 ± 5.1 mg·g<sup>â1</sup> dry weight (D.W.); B. brizantha = 25.0 ± 1.9 mg·g<sup>â1</sup> D.W.) and spring (B. decumbens =
49.4 ± 5.0 mg·g<sup>â1</sup> D.W.; B. brizantha = 27.9 ± 1.4 mg·g<sup>â1</sup> D.W.), although in the case of B. decumbens these values do not vary significantly among seasons
Multidirectional characterisation of chemical composition and health-promoting potential of <i>Rosa rugosa</i> hips
<p>Rugosa rose provides one of the largest hips frequently used in the preparation of pharmaceutical and food products. The aim of work was to conduct multidirectional study of biological activity and chemical composition of <i>Rosa rugosa</i> hips. Antiradical, cytotoxic (against cervical and breast cancer cell lines), antibacterial (against eight bacterial strains) and antifungal potential of the species in question was evaluated. Total contents of phenolics, phenolic acids, flavonoids, tannins, carotenoids and ascorbic acid were determined. LCâESIâMS/MS analysis was performed in order to investigate closely phenolic acids and flavonoid glycosides. As a result, interesting selective cytotoxic effects on cervical (HeLa) and breast cancer (T47D) cell lines, significant antiradical activity (EC<sub>50</sub> 2.45 mg mg<sup>â1</sup> DPPH<sup>âą</sup>) and moderate antimicrobial potential (MIC 0.625â1.25 mg mL<sup>â1</sup>) were observed. Nine phenolic acids and 11 flavonoid glycosides were qualitatively and quantitatively determined, including 7 compounds previously not reported in <i>R. rugosa</i> hips.</p
Triterpenoid Components from Oak Heartwood (<i>Quercus robur</i>) and Their Potential Health Benefits
For
centuries oak wood (<i>Quercus robur</i>) has been
used in aging of wines and spirits, which is based on pleasant flavors
given to beverages by phenolics transferred to the liquid during the
maturation process. Other metabolites, such as triterpenoids, can
also be released. Searching for extractable triterpenoids in oak heartwood,
12 new, <b>1</b>â<b>12</b>, and five known, <b>13</b>â<b>17</b>, oleanane types were isolated and
characterized. Their cytotoxicities were tested against cancer cells
(PC3 and MCF-7) and lymphocytes. Breast cancer cells (MCF-7) were
the most affected by triterpenoids, with roburgenic acid, <b>4</b>, being the most active compound (IC<sub>50</sub> = 19.7 ÎŒM).
Selectivity was observed for compounds <b>1</b>â<b>3</b>, <b>8</b>, <b>9</b>, and <b>16</b>, exhibiting
an IC<sub>50</sub> > 200 ÎŒM against lymphocytes, while active
against cancer cells. A galloyl unit attached to the triterpenoid
moiety was established as the key feature for such effect. These results
highlight the occurrence of triterpenoids in oak heartwood and their
relevance for chemoprevention of breast cancer
Triterpene Saponins from the Aerial Parts of Trifolium medium L. var. <i>sarosiense</i>
Seven
previously unreported triterpene glycosides (<b>1</b>â<b>7</b>) were isolated from methanol extract of the
aerial parts of Trifolium medium var. <i>sarosiense</i> (zigzag clover). Their structures were established
by the extensive use of 1D and 2D NMR experiments along with ESI-MS
and HRMS analyses. Compounds <b>1</b>â<b>7</b> are
oleanane derivatives characterized by the presence of a keto group
at C-22 of an aglycone and a primary alcoholic function at C-24 and
differing functions at C-30. Among these, compounds <b>1</b>â<b>3</b> and <b>6</b> showed a secondary alcoholic
function at C-11, which is methoxylated in compounds <b>4</b> and <b>7</b>. Compound <b>5</b> was shown to possess
a known aglycone, wistariasapogenol A; however, it is described here
for the first time as a saponin constituent of the <i>Trifolium</i> genus. Some aspects of taxonomic classification of zigzag clover
are also discussed