5 research outputs found
Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers
Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11
RAPD primers. In the study RAPD primers detected more polymorphism (98%)
than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR
and 0.85 for RAPD) for each of these marker systems. Cluster analysis
performed from both separate and combined data of RAPD and ISSR markers
using SPSS software package. Jaccard’s similarity coefficient for 62
chickpea genotypes was 0.65. Cluster analyses based on combined data
generated a dendrogram that separated genotypes into 11 clusters. Four
clusters contained only one genotype showing the genetic uniqueness of these
accessions. The studied chickpea collection has been proved to constitute a
rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing
between distantly related genotypes is expected to yield more vigorous
plants constituting much of the different traits contained in the two
parental lines
Assessment of genetic diversity and search for Plum pox virus resistance alleles of apricot<i> (Prunus</i><i> armeniaca</i> L.) genotypes spread in Azerbaijan using SSR markers
Apricot is one of the most important stone-fruit plants widely cultivated in Azerbaijan. SSR markers were used to evaluate the genetic diversity of 61 apricot genotypes representing the entire gene pool in Azerbaijan. A total of 138 alleles were produced based on 17 SSR markers with an average of 8.1 alleles per primer. The polymorphism information content (PIC) and expected (He) heterozygosity for individual loci were in the range of 0.42-0.8 (mean 0.68) and 0.52-0.82 (mean 0.72), respectively, indicating the abundant genetic variation in Azerbaijani apricot cultivars. The polymorphisms generated by SSRs were enough to differentiate 95% of genotypes, while 4 apricot cultivars exhibited complete similarity between them. NJ tree grouped the 61 genotypes into 7 clusters with 1000 bootstrap values, where the grouping of genotypes was not closely related to the geographic origin or collection site. STRUCTURE analysis identified 4 subpopulations in the collection and, in general, was in agreement with cluster analysis. (SEO)/Harlayne-type resistance alleles of three SSR (PGS1.21-240 bp, PGS1.23-161 bp, PGS1.24-119 bp) and one SSLP loci (ZP002-127 bp) were detected in 3, 15, 15, and 9 accessions, respectively. STRUCTURE analysis could separate genotypes with resistance alleles grouping them in the first two subpopulations. Of the 61 apricot genotypes, 28 had at least one resistance allele. The results demonstrate that Azerbaijani apricots are an important source for breeding PPV-resistant apricots
Genetic relationship among introduced lentil germplasm using agronomic traits and ISSR markers
Lentil (Lens culinaris Medik.) is an annual, cool-season grain legume playing
an important role in human and animal nutrition, as soil fertility
maintenance. National lentil improvement program in Azerbaijan is currently
focused on extending the genetic base of the lentil collection through the
introduction of new breeding lines from ICARDA and involving them into
breeding. The present study was aimed to evaluate the performance of lentil
collection, mainly comprised of ICARDA-derived breeding lines for yield
traits under Azerbaijan condition and assess genetic diversity among them
using inter simple sequence repeats (ISSR) markers. Many breeding lines of
ICARDA exhibited agro-morphological performance superior to those of the
local improved varieties. Our studies confirmed that the genetic base of the
studied lentil collection is quite above board. A total of 71 bands were
generated using 7 ISSR primers in 47 lentil genotypes, of which 62 were
polymorphic. Genetic diversity values varied from 0.61 (UBC 848) to 0.95
(UBC 835), with a mean of 0.81. ISSR dendrogram was able to clearly
distinguish all lentil accessions. Clear tendention was observed on
clustering of genotypes according to their pedigree or origin with few
exeptions. The results obtained from the Principal Coordinate Analysis were
consistent with the results of cluster analysis, with minor differences.
Breeding lines with high agronomic performance and sufficient genetic
distance from this study can be used as appropriate parents to get more
heterotic recombinants. This will accelerate the creation of new varieties
well adapted to eco-geographic condition of Azerbaijan with stable and high
yield