The impaired balances of oxidant/antioxidant and COX-1/COX-2 in ovarian ischemia-reperfusion injury and prevention by Nimesulide

Cetin, Nihal/0000-0003-3233-8009; Gul, Mehmet Ali/0000-0002-5849-0116;WOS: 000321086300017The aims of this study were to investigate the association of ovarian I/R injury with oxidant/antioxidant and cyclooxygenase activity and to examine the effect of nimesulide in I/R injury. Rats were divided into four groups: sham group, ischemia-reperfusion group (IR), nimesulide 25 mg/kg group (NIM 25), and nimesulide 50 mg/kg group (NIM 50). the severe oxidative stress and inflammation that occurred in the ovarian tissue treated I/R were recovered by treatment of nimesulide. the histopathological findings, severe haemorrhage, oedema, vascular congestion accompanied with migration and adhesion of polimorphonuclear leukocytes in the endothelium were observed in the IR group that MDA, MPO and COX-2 levels were found high whereas GSH and COX-1 levels were found low. the severe histopathological findings in IR group were moderate in NIM-25 group whereas those were slight in NIM-50 group. This finding suggests that nimesulide prevents injury due to reperfusion following ischemia better when used with dosage 50 mg/kg

Effect of thiamine pyrophosphate on ischemia-reperfusion induced oxidative damage in rat kidney

Hacimuftuoglu, Ahmet/0000-0002-9658-3313; Cetin, Nihal/0000-0003-3233-8009WOS: 000322775000005PubMed: 24014907Objectives: the biochemical effects of thiamine pyrophosphate on ischemia-reperfusion (IR) induced oxidative damage and DNA mutation in rat kidney tissue were investigated, and compared to thiamine. Materials and Methods: Rats were divided into four groups: Renal ischemia-reperfusion (RIR); thiamine pyrophosphate + RIR (TPRIR); thiamine + RIR (TRIR); and sham group (SG). Results: the results of biochemical experiments have shown that malondialdehyde (MDA) levels in rat kidney tissue after TRIR and TPRIR treatment were 7.2 +/- 0.5 (P > 0.05) and 3.3 +/- 0.3 (P 0.05), 5.8 +/- 0.4 (P 0.05), 0.93 +/- 0.1 (P < 0.0001), 0.85 +/- 0.08 (P < 0.0001), and 1.93 +/- 0.24 pmol/L, respectively. Conclusions: It has been shown that thiamine pyrophosphate prevents increase in mutagenic DNA in IR induced oxidative damage, whereas thiamine does not have this effect