Chemical profile and antioxidative activity of red wines obtained from autochthonous and international grape clone varieties

Ova tema je fokusirana na hemijsku i biološku karakterizaciju odabranih uzoraka crvenih vina. Akcenatje stavljen na hemijsku karakterizaciju klonskih sortnih vina vranac i merlo, kao i na procenu antioksidativneaktivnosti merlo vina u ćelijama kvasca. U radu su primenjene brojne analitičke tehnike zaodređivanje koncentracije fenolnih jedinjenja, elementalnog sastava i in vitro antioksidativnu aktivnostiispitivanih uzoraka (spektrofotometrija, ciklična voltametrija, klasična polarografija, EPR spektroskopija,LC-MS/MS, ICP-OES i ICP-MS). Pored antioksidativne, ispitane su i citotoksična i antibakterijska aktivnost odgovarajućihuzoraka vina. Eksperiment na bazi vinskog kvasca je obuhvatio određivanje ukupnih –SHgrupa, kao i enzimskih aktivnosti glutation peroksidaze, glutation reduktaze i katalaze. Eksperimentalnodobijeni podaci za klonska sortna vina merlo ukazuju na poseban doprinos glutation peroksidaze preživljavanjućelija kvasca u uslovima oksidativnog stresa. Konačno, primenom veštačke neuronske mreže razvijenaje brza i precizna metoda za predviđanje uticaja malih promena u vinskom fenolnom profilu naaktivnosti istaknutih enzima antioksidativne zaštite.This topic focuses on the chemical and biological characterization of selected red wine samples. Emphasisis placed on the chemical characterisation of clonal wines of Vranac and Merlot varieties, as well ason the evaluation of the antioxidant activity of Merlot wines in yeast cells. Numerous analytical techniqueswere used to determine the concentration of phenolic compounds, elemental composition and invitro antioxidant activity of the tested samples (spectrophotometry, cyclic voltammetry, classical polarography,EPR spectroscopy, LC-MS/MS, ICP-OES and ICP-MS). In addition, total antioxidant activity, cytotoxicand antibacterial activity of the respective wine samples were determined. The wine yeast experimentinvolved determining the total groups –SH, as well as enzymatic activities of glutathione peroxidase, glutathionereductase and catalase. The experimentally obtained data for clonal Merlot wine varieties indicatea particular contribution of glutathione peroxidase to the survival of yeast cells under oxidative stress conditions.Finally, using an artificial neural network (ANN), a rapid and accurate method was developed to predictthe effect of small changes in the wine phenolic profile on the activities of prominent antioxidantenzymes

The effects of a selected methoxy substituted chalcone in human melanoma cells irradiated with γ-rays

Given the well-established potential of chalcones in modulating the response of cancer cells to therapeutic interventions, coupled with the growing imperative to enhance their biological attributes, the objective of this study was to synthesize a methoxy-substituted chalcone (OCH3) and assess its capacity to amplify the inhibitory effects of radiation in melanoma cells known for their resistance to radiotherapy. The A375 melanoma cells were subjected to a clinically relevant dose of 2 Gy gamma irradiation. OCH3 was employed either as a standalone treatment or in conjunction with irradiation. The obtained results unveiled the substantial radiosensitizing potential of OCH3 within this specific cell line. Our subsequent investigations will be designed to investigate the underlying mechanisms that contribute to the radiosensitizing properties of OCH3. Moreover, we intend to evaluate the efficacy of OCH3 against other types of radioresistant cancer cells. The presented data not only illuminates the enhanced therapeutic possibilities offered by OCH3 but also highlights its potential as a valuable agent in addressing a wider array of challenging malignancies.ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023; Kragujeva

Supplementary material for: "Multielement analysis and antioxidant capacity of Merlot wine clones developed in Montenegro"

Experimental details: Standards and solvents; Wine samples; Multielement analysis; Cyclic voltammetric determination of antioxidant capacity. Additional references. Supplementary Figure: Cyclic voltammograms obtained for the analysed wine samples during 2010 (a) and 2011 (b) vintages. Table S1. The contents of major and trace elements in analysed wine samples determined by ICP-OES or ICP-MS. Table S2. The content of ultratrace elements in analysed wine samples determined by ICP-MS. Table S3. Antioxidant potency composite index (ACI) of the selected wine samples determined 9 by cyclic voltammetry and scaled to relative percentages.This is supplemetray material for: Đorđević, N. O., Pejin, B., Novaković, M., Stanković, D., Mutić, J., Pajovic, S. B.,& Tešević, V. (2018). Multielement analysis and antioxidant capacity of Merlot wine clones developed in Montenegro. Natural Product Research, Taylor & Francis, 32(3), 247-251. [https://doi.org/10.1080/14786419.2017.1347171]The published version of the article: [https://cer.ihtm.bg.ac.rs/handle/123456789/2456

In vitro Radioprotective Activity of the Bryozoan Hyalinella punctata

The objective of the present study was in vitro evaluation of radioprotective activity of the freshwater bryozoan Hyalinella punctata on cultured human peripheral blood lymphocytes after irradiation with 2 gy of Co-60 gamma-rays. Since its water extract at concentration 0.001 mg/mL reduced the incidence of radiation-induced micronuclei for almost 30 %, it could be considered as a promising source of new natural products with the aforementined activity. Both the content of sulphur (1.17%) determined by gravimetric method and infrared absorption frequences (76 % similarity with those of bacitracin) of the investigated extract indicate the presence of organic sulphur compound(s) such as cyclic peptides and polypeptides which might be responsible for the observed radioprotectio

Olanzapine intensifies lipid peroxidation and modulates catalase activity in liver of social isolated rats

Olanzapine is an antipsychotic proved to be effective in stress associated psychiatric diseases, but its effect on the liver, main site of drug metabolism,still remain unclear. We investigated the effects of chronic treatment of olanzapine (three-week) on the malondialdehyde (MDA) content and protein expression and activity of antioxidant enzyme catalase (CAT) in the liver of rats exposed to chronic social isolation (CSIS) (six-week), an animal model of depression. The increased cytosolic MDA content in both vehicle-and olanzapine-treated CSIS animals suggests oxidative stress. Increased CAT activityin vehicle-treated CSIS animals, which was not consistent with its protein expression, suggests induction of antioxidant defense mechanisms,while olanzapine significantly reduced CAT activity in CSIS group. Data revealed that although olanzapine treatment reversed the alterations in CAT activity, it has the ability to cause hepatotoxicity, as indicatedby increased MDA content.Physical chemistry 2016 : 13th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 26-30 September 2016

Supplementary data for article: Đorđevic, N. O.; Novaković, M. M.; Pejin, B.; Mutić, J.; Vajs, V.; Pajovic, S. B.; Tešević, V. Comparative Analytical Study of the Selected Wine Varieties Grown in Montenegro. Natural Product Research 2017, 31 (15), 1825–1830. https://doi.org/10.1080/14786419.2017.1289209

Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2481]Supplementary material for: [https://doi.org/10.1080/14786419.2017.1289209

Supplementary data for the article: Đorđević, N.; Novaković, M.; Pejin, B.; Živković, M.; Savić, A.; Mutić, J.; Tešević, V. An Insight into Chemical Composition and Biological Activity of Montenegrin Vranac Red Wine. Scientia Horticulturae 2018, 230, 142–148. https://doi.org/10.1016/j.scienta.2017.11.033

Supplementary material for: [https://doi.org/10.1016/j.scienta.2017.11.033]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2072

Supplementary material for the article: Đorđević, N. O.; Pejin, B.; Novaković, M. M.; Stanković, D. M.; Mutić, J. J.; Pajović, S. B.; Tešević, V. V. Multielement Analysis and Antioxidant Capacity of Merlot Wine Clones Developed in Montenegro. Nat. Prod. Res. 2018, 32 (3), 247–251. https://doi.org/10.1080/14786419.2017.1347171

Supplementary material for: [https://www.tandfonline.com/doi/abs/10.1080/14786419.2017.1347171?journalCode=gnpl20]Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2563

Synthesis and cytotoxic activity of selected dual COX-2 and 5-LOX inhibitors in HeLa and MIA PaCa-2 human cancer cell lines

Among novel cancer chemotherapy approaches, the use of cyclooxygenases (COXs) and lipoxygenases (LOXs) inhibitors represents a promising mean for cancer treatment showing lesser toxicity comparing to the currently used cytotoxic drugs. This study detailed the synthesis of three novel compounds: 1ME, BHTK-AA, and IBU-Ac, each with the capability to concurrently inhibit both COX-2 and 5-LOX. Subsequently, we assessed their effectiveness in inhibiting the proliferation of HeLa cervical and MIA PaCa-2 pancreatic cancer cells. The IC50 values for both examined cell lines were approximately 40 μM, indicating the promising inhibitory potential of the IBU-Ac compound in both types of cancer cells. This finding is positioned to stimulate further investigation into the potential application of IBU-Ac against these particular types of cancers, while also advocating its use in combination with standard anti-cancer protocols, i.e., chemoterapeutics or radiation therapy. The results of this work are also advocating the development and refinement of dual COX-2 and 5-LOX inhibitors, thus improving their efficacy and safety.ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023; Kragujeva

Supplementary data for article: Đorđevic, N. O.; Novaković, M. M.; Pejin, B.; Mutić, J.; Vajs, V.; Pajovic, S. B.; Tešević, V. Comparative Analytical Study of the Selected Wine Varieties Grown in Montenegro. Natural Product Research 2017, 31 (15), 1825–1830. https://doi.org/10.1080/14786419.2017.1289209

Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/2481]Supplementary material for: [https://doi.org/10.1080/14786419.2017.1289209