BIOKEMIJSKI I HEMATOLOŠKI POKAZATELJI I INDEKS TJELESNE KONDICIJE KRAVA HOLSTEINSKE PASMINE S OBZIROM NA OCJENU DOBROBITI

Animal welfare (AW) is a term denoting how the animals are coping with the conditions in which they live. A precise welfare assessment assumes a multidisciplinary approach, and modern evaluation protocol incorporates both the animal-based and non-animal-based measures. Due to the different welfare score classes, this study’s objective was to determine the variability of biochemical and hematological parameters, as well as the body condition score. The study was conducted involving 145 Holstein cows, randomly chosen from the six commercial dairy cow farms. The dairy cows’ welfare assessment checklist consisted of seventy items, pursuant to the CReNBA protocol. The biochemical parameters in blood and the milk plasma were determined using the Beckman Coulter AU400 (Beckman Coulter, FRG) automatic clinical chemistry analyzer. The obtained results indicate that the lower albumin, triglyceride, iron, and calcium values were detected in the cows bred on the farms that were ranked lower concerning the farm management and farm infrastructure levels. In the milk plasma, the concentration of albumins was higher (P<0.05) on the farms that were ranked lower concerning the farm management, infrastructure level, and total score scale. Iron concentration was higher (P<0.05) in the cows from the farms ranked lower concerning farm infrastructure and on the farms that were ranked higher concerning the animal level. The erythrocyte sedimentation rate was higher on the farms with worse marks on the farm infrastructure level and total AW score.Dobrobit životinja predstavlja procjenu stanja životinja kojima reagiraju na podražaje iz okoline. Precizna procjena dobrobiti podrazumijeva multidisciplinaran pristup, a suvremen evaluacijski protokol uključuje mjerenja na životinjama i mjerenja koja uključuju smještaj, hranidbu i upravljanje farmom. Svrha ovoga istraživanja bila je odrediti varijabilnost biokemijskih i hematoloških pokazatelja, kao i tjelesnu kondiciju, s obzirom na različite bodovne razrede dobrobiti. Istraživanje je provedeno na 145 krava holsteinske pasmine, nasumično odabranih na šest komercijalnih farma mliječnih krava. Kontrolni popis procjene dobrobiti mliječnih krava sastojao se od 70 pitanja prema protokolu CReNBA. Biokemijski pokazatelji u krvi i mliječnoj plazmi određeni su automatskim kemijskim analizatorom Beckman Coulter AU400 (Beckman Coulter, SRNj). Dobiveni rezultati ukazuju da niže koncentracije albumina, triglicerida, željeza i kalcija u krvi imaju krave na lošije ocijenjenim farmama s obzirom na menadžment farme i infrastrukturu. S obzirom na pokazatelje infrastrukturnih karakteristika farme, menađmenta i ukupnu ocjenu farme, koncentracija albumina u mliječnom serumu bila je veća (P<0.05)u krava s lošijom ocjenom. Sedimentacija eritrocita bila je brža na lošije ocijenjenim farmama s obzirom na infrastrukturu farme i ukupnu ocjenu dobrobiti

Certain dietary minerals and udder health in dairy cows

Mastitis, upala mliječne žlijezde jedna je od tri glavne bolesti koje utječu na profitabilnost proizvođača mlijeka i najskuplja bolest intenzivnog mliječnog govedarstva. Ekonomski gubici nastaju zbog odbacivanja mlijeka, povećanog izlučenja krava, troškova lijekova, rada veterinara i rada samih stočara. Međutim, sve je više dokaza da hranidba može imati značajan učinak na imunosni sustav, čime utječe na incidenciju infekcije i tijek mastitisa. Glavni utjecaj hranidbe na zdravlje vimena je putem supresije imunosnog sustava. Loš sastav obroka ne uzrokuje mastitis, ali može olakšati bakterijama da se udomaće u mliječnoj žlijezdi, što rezultira povećanom stopom mastitisa. Mliječna žlijezda krava često je izložena potencijalnim patogenima, ali većina krava ne dobije mastitis jer je njihov imunosni sustav adekvatan da spriječi infekciju. Cilj ovog rada je rasvijetliti povezanost između određenih minerala iz hrane i mastisa u mliječnih krava. Minerali dokazano imaju učinak na proizvodnju i reprodukciju, a njihov nedostatak može rezultirati imunosupresijom. Minerali koji utječu na zdravlje vimena su: selen, bakar, cink, kalcij, fosfor, magnezij, sumpor, mangan i molibden.Mastitis, an inflammation of the mammary gland, is one of the three main diseases that affect the profitability of milk producers and the most expensive disease of intensive dairy cattle farming. Economic losses occur due to the rejection of milk, increased excretion of cows, the cost of medicines, the work of veterinarians, and the work of the herdsmen themselves. However, there is increasing evidence that nutrition can have a significant effect on the immune system, thereby influencing the incidence of infections and the course of mastitis. The main influence of feeding on udder health is through the suppression of the immune system. A poor diet does not cause mastitis, but it can make it easier for bacteria to colonize the mammary gland, resulting in an increased rate of mastitis. The mammary gland of cows is often exposed to potential pathogens, but most cows do not develop mastitis because their immune systems are adequate to prevent infection. This work aims to describe the relationship between certain minerals from food and mastitis in dairy cows. Minerals have been proven to affect production and reproduction, and their deficiency can result in immunosuppression. Minerals that affect the health of the udder are selenium, copper, zinc, calcium, phosphorus, magnesium, sulfur, manganese, and molybdenum

Certain dietary minerals and udder health in dairy cows

Mastitis, upala mliječne žlijezde jedna je od tri glavne bolesti koje utječu na profitabilnost proizvođača mlijeka i najskuplja bolest intenzivnog mliječnog govedarstva. Ekonomski gubici nastaju zbog odbacivanja mlijeka, povećanog izlučenja krava, troškova lijekova, rada veterinara i rada samih stočara. Međutim, sve je više dokaza da hranidba može imati značajan učinak na imunosni sustav, čime utječe na incidenciju infekcije i tijek mastitisa. Glavni utjecaj hranidbe na zdravlje vimena je putem supresije imunosnog sustava. Loš sastav obroka ne uzrokuje mastitis, ali može olakšati bakterijama da se udomaće u mliječnoj žlijezdi, što rezultira povećanom stopom mastitisa. Mliječna žlijezda krava često je izložena potencijalnim patogenima, ali većina krava ne dobije mastitis jer je njihov imunosni sustav adekvatan da spriječi infekciju. Cilj ovog rada je rasvijetliti povezanost između određenih minerala iz hrane i mastisa u mliječnih krava. Minerali dokazano imaju učinak na proizvodnju i reprodukciju, a njihov nedostatak može rezultirati imunosupresijom. Minerali koji utječu na zdravlje vimena su: selen, bakar, cink, kalcij, fosfor, magnezij, sumpor, mangan i molibden.Mastitis, an inflammation of the mammary gland, is one of the three main diseases that affect the profitability of milk producers and the most expensive disease of intensive dairy cattle farming. Economic losses occur due to the rejection of milk, increased excretion of cows, the cost of medicines, the work of veterinarians, and the work of the herdsmen themselves. However, there is increasing evidence that nutrition can have a significant effect on the immune system, thereby influencing the incidence of infections and the course of mastitis. The main influence of feeding on udder health is through the suppression of the immune system. A poor diet does not cause mastitis, but it can make it easier for bacteria to colonize the mammary gland, resulting in an increased rate of mastitis. The mammary gland of cows is often exposed to potential pathogens, but most cows do not develop mastitis because their immune systems are adequate to prevent infection. This work aims to describe the relationship between certain minerals from food and mastitis in dairy cows. Minerals have been proven to affect production and reproduction, and their deficiency can result in immunosuppression. Minerals that affect the health of the udder are selenium, copper, zinc, calcium, phosphorus, magnesium, sulfur, manganese, and molybdenum

Hematološki i biokemijski pokazatelji izvorne pasmine hrvatska bijela koza različite dobi

The aim of the research was to determine the age-related hematological and biochemical parameters in Croatian white goats in a traditional Mediterranean production system. The 36 lactating Croatian white goats were divided into three groups with 12 goats each, according to age: group I (1-2 years old), group II (3-5 years) and group III (>5 years). The mean corpuscular haemoglobin concentration (334 g/L) and lymphocytes (73.71%), as well as the concentration of iron (Fe 25.17 μmol/L) in the blood were higher, while the concentration of urea (3.86 mmol/L) was lower in group I compared to group III. The results of the present study are the first published blood analysis (hematological and biochemical parameters) for the Croatian white goat breed, and can be useful for understanding the metabolism and health status of this breed of goats, which helps in their conservation, promotion and breeding improvement.Cilj istraživanja bio je utvrditi hematološke i biokemijske pokazatelje koza različite dobi, pasmine hrvatska bijela koza. Koze su uzgajane u tradicionalnom mediteranskom proizvodnom sustavu. Ukupno 36 koza u laktaciji podijeljeno je u tri skupine, svaka s po 12 koza, prema dobi: skupina I (dob 1-2 godine), skupina II (dob 3-5 godina) i skupina III (dob > 5 godina). Prosječna koncentracija hemoglobina u eritrocitima (334 g/l) i udio limfocita (73,71%), kao i koncentracija željeza (Fe 25,17 μmol/L) u krvi bile su više, dok je koncentracija uree (3,86 mmol/l) bila niža u skupini I u usporedbi sa skupinom III. Rezultati ovog istraživanja su prva objavljena analiza krvi (hematološki i biokemijski pokazatelji) za hrvatsku bijelu kozu. Utvrđene vrijednosti mogu biti korisne za razumijevanje metabolizma i zdravstvenog statusa ove pasmine koza, što pomaže u njezinom očuvanju, promociji i poboljšanju uzgoja

DEA 1.1. krvne grupe hrvatskih autohtonih pasmina pasa: posavskog goniča i tornjaka.

The study included 60 clinically healthy dogs of two Croatian indigenous breeds: the Posavaz Hound and the Tornjak Hound. Their ages ranged from 2 months to 8 years. Determination of blood group DEA 1.1. (Dog Erythrocyte Antigen) was performed using tests based on serological agglutination reaction. The blood of dogs that showed a positive reaction to the blood group DEA 1.1., intensity of agglutination (low, medium and strong) was determined. The Posavaz Hound breed showed 60% of dogs positive for DEA 1.1. group, and the Tornjak Hound breed showed 53.3% positive. In the Tornjak Hounds the strongest positive reaction was found for the largest number of individuals (56.3%), while in the Posavaz Hound breed for most individuals the lowest positive response was determined (55.6%). The strongest intensity of reaction was found in Tornjak Hound females and the lowest in Posavaz Hound males. Using Kruskal-Wallis and Chi-square tests no statistical difference was determined between the positive reaction intensity of Posavaz and Tornjak Hound DEA 1.1. blood groups.Istraživanjem je bilo obuhvaćeno 60 klinički zdravih pasa dviju hrvatskih autohtonih pasmina: posavski gonič (15 ženki i 15 mužjaka u dobi od 7 mjeseci do 8 godina) i tornjak (18 ženki i 12 mužjaka u dobi od 2 mjeseca do 7 godina). Uzorak krvi uzet je iz krvne žile v. cephalica u epruvete s EDTA antikoagulansom. Nakon vađenja krvi određena je moguća reakcija autoaglutinacije, koja je u svim uzorcima bila negativna. Određivanje krvne grupe DEA 1.1. (Dog Erythrocyte Antigen) obavljeno je pomoću serološkog testa RAPIDVET®-H (Canine DEA 1.1., dms/agrolabo products ag Neuhausen am Rheinfall, Switzerland), temeljenog na serološkoj reakciji aglutinacije koja nastupa kada eritrociti pasa koji sadrže DEA 1.1. antigen dođu u dodir s mišjim monoklonskim protutijelima. U krvi pasa koji su pokazali pozitivnu reakciju na krvnu grupu DEA 1.1., određena je jakost aglutinacije (slaba, srednja jak i jaka). Utvrđeno je 60% pozitivnih pasa na DEA 1.1. grupu u posavskog goniča, a 53,3% u tornjaka. Jaka reakcija zabilježen je u 56,3% pasa pasmine tornjak, dok je većina pozitivnih pasa pasmine posavski gonič imala slabu reakciju (55,6%). Najjača reakcija utvrđena je u ženki tornjaka, a najslabija u mužjaka posavskog goniča. Primjenom Kruskal-Wallis i Hi kvadrat testa nije utvrđena statistički značajna razlika

Procjena posmortalnog intervala u jelena običnog (Cervus elaphus) na temelju biokemijskih vrijednosti staklovine korištenjem linearne regresijske analize

Vitreous fluid is a stable biological fluid, which is well-protected from contamination and post mortem degradation, which makes it suitable in forensic pathology for determination of the post mortem interval (PMI). The present study was conducted to discover the correlation between increasing PMI and levels of various vitreous biochemical parameters (sodium, calcium, magnesium, chloride, potassium, phosphor, AST, urea, creatinine, total protein, glucose, total cholesterol). The estimation of PMI in red deer is of great interest in cases of illegal hunting. The vitreous humour samples were collected from six red deer carcasses. The eyes were enucleated, covered by parafilm and aluminium foil, packed in a small container and maintained at +4 ºC. Sampling of vitreous fluid was done again at 8, 10 and then regularly each 10 hours until finally 90 hours after death. A significant linear correlation was found for potassium, sodium and chloride (P<0.0001). The present study revealed that biochemical analysis of vitreous fluid could be used to determine post mortem interval in red deer.Staklovina je stabilna biološka tekućina koja je dobro zaštićena od kontaminacije i postmortalne degradacije što je čini pogodnom u forenzičkoj patologiji za određivanje postmortalnog intervala (PMI). Ovo je istraživanje provedeno kako bi se utvrdila korelacija između povećanja (PMI) i razina različitih biokemijskih parametara staklovine (natrij, kalcij, magnezij, klorid, kalij, fosfor, AST, urea, kreatinin, ukupni protein, glukoza, ukupni kolesterol). Procjena PMI-ja u jelena običnog od velikog je interesa za slučajeve protuzakonitog lova. Za potrebe istraživanja staklovina je prikupljena od šest odstrijeljenih jelena. Nakon što su oči izvađene, obložene su parafilmom i aluminijskom folijom te smještene u posudice pohranjene na stalnu temperaturu od +4 ºC. Staklovina je prikupljena 8., 10., a zatim redovito svakih 10 sati do, konačno, 90. sata nakon smrti. Linearnom regresijskom analizom utvrđena je povezanost PMI-ja s kalijem, natrijem i kloridima (P<0,0001). Ovo istraživanje pokazalo je da bi se biokemijska analiza staklaste tekućine mogla koristiti za određivanje PMI-ja kod jelena običnog

Metabolički profil i acidobazna ravnoteža krvi koza u laktaciji i njihove jaradi

The aim of the present research was to determine the blood metabolic profile and acid-base balance of dairy goats and of their kids during lactation. Analyses were made of 15 Alpine goats and their suckling kids, in a traditional production system. The goats’ blood was collected from the 20th to the 140th day of lactation every 30th day. Kids´ blood was collected on the 20th and 50th days of life. From the blood samples the values of pH, partial pressure of carbon dioxide (pCO2), partial pressure of oxygen (pO2), total pressure of carbon dioxide (tCO2) and bicarbonate (HCO3-) were determined. Afterwards, the blood samples collected from the goats and kids were centrifuged, and from the plasma obtained the concentrations of calcium, phosphorus-inorganic, potassium, sodium, iron, chloride, urea, glucose, total proteins, albumin, creatinine, cholesterol, HDL-cholesterol, LDL-cholesterol, trygliceryds, alkaline phosphatase (ALP), creatine kinase (CK) and γ-glutamyl transferase (GGT) were assessed. Also, the strong ion difference (SID), anion gap (AG) and z-values were determined from the blood of the goats and kids. The results were obtained by MEANS procedure, and differences between the groups were determined using ANOVA repeated measures. A significant decrease in glucose, tryglicerides, Na and Fe concentrations was determined, as well as the AG and SID content in the blood, and increases in cholesterol, HDL-cholesterol, LDL-cholesterol, total protein, albumin, globulin and P-inorganic in the blood of goats during lactation. The concentrations of Cl, creatinine, and enzymatic activity (CK, GGT and ALP) in the blood of goats during lactation did not differ significantly. The results of the blood metabolic profile and acid-base balance indicate the need for better control of goats and kids´ rations in traditional production systems.Cilj ovog istraživanja bio je utvrditi metabolički profil i acidobaznu ravnotežu krvi mliječnih koza u laktaciji, kao i njihove jaradi. Pokus je proveden na 15 koza pasmine francuska alpina i njihove sisajuće jaradi u tradicionalnom sustavu uzgoja. Uzorkovanje krvi koza provedeno je od 20. do 140. dana laktacije, svakih 30 dana. Uzorkovanje krvi jaradi provedeno je u dobi od 20 i 50 dana. U krvi su utvrđene vrijednosti pH, parcijalni tlak ugljikova dioksida (pCO2), parcijalni tlak kisika (pO2), ukupni tlak ugljikova dioksida (tCO2) i sadržaj bikarbonata (HCO3). U plazmi dobivenoj centrifugiranjem uzoraka krvi koza i jaradi utvrđene su koncentracije kalcija, fosfora-anorganskog, kalija, natrija, željeza, klora, ureje, glukoze, ukupnih proteina, albumina, kreatinina, kolesterola, HDL-kolesterola, LDL-kolesterola, triglicerida, alkalne fosfataze (ALP), kreatin kinaze (CK) i γ-glutamil transferaze (GGT). U krvi koza i jaradi utvrđena je razlika jakih iona (SID), anionski procjep (AG) te z-vrijednost. Rezultati su analizirani MEANS procedurom, a razlike između skupina utvrđene su procedurom ANOVA-repeated measures. U krvi koza u laktaciji utvrđeno je značajno smanjenje koncentracije glukoze, triglicerida, Na i Fe, kao i sadržaj AG i SID te povećanje kolesterola, HDL-kolesterola, LDL-kolesterola, ukupnih proteina, albumina, globulina i P-anorganski. U krvi koza u laktaciji nisu utvrđene značajne razlike koncentracije Cl, kreatinina i aktivnosti enzima (kao što su CK, GGT i ALP). Rezultati metaboličkog profila i acidobazne ravnoteže ukazuju na potrebu praćenja kvalitete obroka koza i jaradi u tradicionalnom sustavu proizvodnje

The influence of the addition of dietary selenium on the haematological and biochemical parameters in fallow deer (Dama dama L.)

Selen je esencijalni element uključen u brojne fiziološke procese te je potrebno i u domaćih i u divljih životinja putem hrane osigurati njegov unos. Oblik selena utječe na njegovu bioraspoloživost i dostupnost u sintezi selenoproteina. Upravo je stoga neophodan, jer kao dio enzimskih sustava sudjeluje u metabolizmu štitaste žlijezde, ključan je u reprodukciji i dijelom je važnog antioksidacijskog sustava. Cilj istraživanja bio je ustvrditi utjecaj dodatka organskog oblika selena (Selplex®, Alltech, SAD) u krmnu smjesu za jelene u količini od 0,5 mg/ kg suhe tvari na hematološke i biokemijske pokazatelje u jelena lopatara. Istraživanje je provedeno na 40 jelena lopatara tijekom dvije godine istraživanja, na po dvadeset svake godine. Životinje smo podijelili na mlade (do 1 godine starosti) i odrasle (2-9 godina). Krmna smjesa ponuđena je tijekom 60 dana na kraju ljetnog perioda godine. Utvrđen je trend povećanja ukupnih proteina, globulina i imunoglobulina G klase u odraslih jedinki hranjenih s većim udjelom selena u hrani, ali bez statističke značajnosti. Koncentracija LDL kolesterola bila je značajno (P<0,05) veća u odraslih jelena hranjenih s većom koncentracijom selena. Pad koncentracije željeza u jelena kojima je dodavan selen znakom je da su životinje uistinu dobile veću količinu selena u obroku nego prve godine. S obzirom da nije bilo značajnih razlika u rezultatima hematoloških i biokemijskih pokazatelja, moguće je preispitati normative za potrebne koncentracije selena kao dodatka u krmnu smjesu za jelene.Selenium is an essential element that is involved in numerous physiological processes and accordingly, its inclusion in the feed of both domestic and game animals is advised. The form of selenium affects its bioavailability and the availability of seleno-aminoacids for the synthesis of selenoproteins. As part of the enzymatic system, it participates in thyroid gland metabolism, and plays an important role in the functioning of the antioxidation system. The aim this study was to determine the influence of organic selenium (Selplex®, Alltech, USA) in the amount of 0.5 mg/kg DM in feed mixture on the hematologic and biochemical indicators of fallow deer. The study was conducted on 40 deer over a two- year period. Animals were divided into two groups: young up to 2 years and adults 2-9 years old. The feed mixture was offered for 60 days at the end of the summer season. The trend of increasing the total protein, globulin and immunoglobulin G levels in adult deer fed with a selenium supplement was established, but without significance. The concentration of LDL cholesterol was significantly (P<0.05) higher in adult deer fed selenium supplement. The drop in iron concentrations in the second year is a sign that animals actually received a higher amount of selenium than in the first year. Given that there were no significant differences in the results of hematological and biochemical indicators, re-examination of the normative for the required selenium concentrations as a supplement in the feed mixture is recommended

Kovarijabilnost svojstava dnevne mliječnosti te hematoloških i biokemijskih parametara u serumu i mlijeku krava holstein pasmine

Aiming to determine the variability and co-variability of daily production traits and biochemical as well as haematological parameters, blood and milk were taken from 25 Holstein cows during a three-month period, on a monthly basis. Thereby differences in analysed parameters due to the animal’s health status (acidosis or ketosis risk or normal health status; defined accordingly to fat/protein ratio) were determined. According to the statistical analysis, the co-variability level varied depending on the set of the analysed traits (from negligible to strong). The determined differences between the analysed biochemical and haematological parameters due to the animal\u27s health status were not statistically significant. The obtained results indicate that the analysed biochemical and haematological parameters cannot be used as accurate indicators of acidosis prevalence, while for indication of ketosis prevalence, glucose and urea content in blood, sedimentation after 24 hours, as well as protein and iron content in milk, could be used. Furthermore, test-day records could be used as a valuable indicator of the prevalence risk of metabolic disorders (acidosis/ketosis) but for unambiguous detection in animals with the indication of a prevalence risk, other diagnostic methods should be used.S ciljem utvrđivanja varijabilnosti i kovarijabilnosti dnevnih proizvodnih svojstava te biokemijskih i hematoloških parametara, uzorkovani su krv i mlijeko 25 krava holstein pasmine tijekom tromjesečnog razdoblja. Također, utvrđene su razlike u analiziranim parametrima uslijed zdravstvenog statusa životinje (rizik od acidoze ili ketoze ili normalno zdravstveno stanje; definirano prema omjeru mliječna masti/protein). Statistička analiza je pokazala da razina kovarijabilnosti varira ovisno o skupu analiziranih svojstava (i to od zanemarive do jake). Razlike između analiziranih biokemijskih i hematoloških parametara uslijed zdravstvenog statusa životinje nisu bile statistički značajne, ali su prisutne. Dobiveni rezultati pokazuju da se analizirani biokemijski i hematološki parametri ne mogu koristiti kao točan pokazatelj pojavnosti acidoze, dok se za indikaciju pojavnosti ketoze može koristiti sadržaj glukoze i uree u krvi, sedimentacija nakon 24 sata te sadržaj proteina i željeza u mlijeku. Nadalje, rezultati kontrole mliječnosti mogu se koristiti kao vrijedan pokazatelj rizika pojavnosti metaboličkih poremećaja (acidoza/ketoza), ali bi se druge dijagnostičke metode trebale koristiti za nedvosmisleno utvrđivanje kod životinja kod kojih rezultati kontrole mliječnosti ukazuju na rizik pojavnosti

BLOOD ACID-BASE BALANCE IN GROWING LAMBS

Cilj je ovoga rada istražiti promjenu pokazatelja acidobazne ravnoteže krvi janjadi u porastu. Istraživanje je provedeno na 10 janjadi Merinolandšaf pasmine prosječne dobi 23. i 63. dana. Janjad je odabrana ravnomjerno prema spolu (50% ♀ : 50% ♂), bila je zdrava i u dobroj kondiciji. Osim što je sisala majke, janjad je imala na raspolaganju obrok koji se sastojao od krmne smjese, ječma i sijena lucerne kojeg su kao i vodu imali na raspolaganju po volji. U krvi janjadi utvrđeni su pH vrijednost, hematokrit, plinovi (pCO2, pO2), elektroliti (Ca, Na+, K+, Cl- ), bikarbonati (HCO3-) i zasićenost kisikom (O2 – sat.) te izračunati z – vrijednost, BEfw, BECl, BEalb, anionski procjep (AG) i razlika jakih iona (SID). Većina pokazatelja acidobazne rav¬noteže krvi bila je unutar referentnih vrijednosti za ovce i janjad, osim blagog odstu¬panja u koncentraciji Ca, Cl, pCO2, O2-sat. Navedene promjene su znak intenzivnih metaboličkih procesa i prilagodbe janjadi na drugačiju strukturu obroka u kojem raste udio krutih i voluminoznih krmiva te rasta i razvoja janjadi. Utvrđivanjem pokazatelja acidobazne ravnoteže krvi, osobito u stresnom razdoblju prijelaza sa sisanja na krutu hranu omogućuje nam uvid u opskrbu janjadi hranjivim tvarima, ukazuje na kvalitetu obroka i njihov zdrastveni status.The aim of this study was to investigate the changes of blood acid base indicators in growing lambs. The research was conducted on the same 10 lambs of Merinolandschaf breed, average age of 23 and 63 days. The lambs were selected evenly by sex (50% ♀: ♂ 50%), were healthy and in good physical condition. Apart from sucking their mothers, lambs had a ration consisting of feed mixture, barley and alfalfa hay, as well as water available ad libitum. In the blood of lambs pH, Hct, gases (pCO2, pO2), electrolytes (Ca, Na+, K+, Cl-and HCO3-), oxygen saturation (O2 - sat.) were determined, and z- value, BEfw , BECl-, BEalb, anion gap (AG) and the strong ion difference (SID) calculate. Most indicators of acid-base balance were within the reference range, except for slight variations in the concentration of Ca, Cl-, pCO2, O2- sat. These changes are a sign of intense metabolic processes and adaptation of lambs to a different structure of ration in which share of solid feed increases. Determination indicators of acid-base balance in the blood of lambs, especially in the stressful period of transition from suckling to solid feed allow an insight into the lambs’ supply of nutrients, indicating the quality of the ration, and lambs’health status