CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF LACTULOSE AND MANNITOL RATIO FOR ESTIMATING INTESTINAL PERMEABILITY

In this study, a simple, rapid and accurate capillary electrophoresis method has been developed for the determination of lactulose and mannitol in urine for estimating the intestinal permeability. The urine samples were prepared to analyze by using solid phase extraction. For analysis a background electrolyte (BGE) consisted of 6 mmol/L sorbic acid, 1.25mmol/L hexadecyltrimeth ylammoniumbromide (HDTMA), 50 mmol/L LiOH (pH 12.5) was used. Separation was performed at -5 kV over 20 min within the fused capillary column (45 cm, 50 mu m I.D.) at 20 degrees C. The method developed was applied a series of urine sample to measure the lactulose/mannitol concentration rate

SPECTROPHOTOMETRIC DETERMINATION OF MIRTAZAPINE IN PURE AND TABLET PHARMACEUTICAL PREPARATION

Sensitive spectrophotometric method was developed for the determination of mirtazapine from pharmaceutical preparats. The proposed method was based on the formation of a yellow colored ion-pair complex by the reaction of methyl orange with mirtazapine. The ion pair complex was extracted with dichloromethane. Under the optimum condition, the ion pair complex showed an absorption maximum at 427 nm. The linearity range for concentrations of mirtazapine was found to be 2.5-12.5 mu g/mL. The proposed method is successfully applied to the determination of mirtazapine tablet formulations