Investigation of possible effect of carnosic acid (CA) on combined exposure to bisphenol a (BPA) and diethyl hexyl phthalate (DEHP)

54th Congress of the European-Societies-of-Toxicology (EUROTOX) - Toxicology Out of the Box -- SEP 02-05, 2018 -- Brussels, BELGIUMWOS: 000454045100375DEHP and BPA, the main components of plastic products, are the important endocrine-disrupting chemicals which can mimic or disrupt the actions of estrogen by inhibiting normal functions of the body. Because of this widespread usage of DEHP and BPA, not only alone exposure but also combined exposure is considered as an important issue. Nowadays, researches are increased related to the effects on neurological functions instead of endocrine disrupting effects. This study aims to investigate combined exposure of DEHP and BPA and the possible effect of carnosic acid as an antioxidant.European Soc Toxico

A pilot study on effects of combination usage of curcumin and melatonin on cisplatin treatment

52nd Congress of the European-Societies-of-Toxicology (EUROTOX) -- SEP 04-07, 2016 -- Seville, SPAINWOS: 000402436700390Cisplatin is an antitumor drug widely used in the treatmentof many malignant tumors. And also some studies show thatmelatonin and curcumin have antitumor activity via several mech-anisms including theirs in addition to theirs potent antioxidantactions. This study was performed to evaluate the effect of cur-cumin and melatonin on the cytotoxicity induced by cisplatin inthe human breast cancer cell line (MCF-7). The cells were treatedwith melatonin or cisplatin or curcumin for 24 and 48 h. Cellviability was quantified using the 3-(4,5-dimethyl-2-thiazolyl)-European Society of Toxicologi

Synthesis of novel methyl jasmonate derivatives and evaluation of their biological activity in various cancer cell lines

Warburg hypothesized that the energy consumption of cancer cells is different than the normal cells. When compared to normal conditions, cancer cells do not undergo tricarboxylic acid (TCA) cycle therefore resulting in more lactate in the cells. Glycolysis pathway is a way of cancer cells to provide energy. The first step in glycolysis is the phosphorylation of glucose to glucose-6-phosphate. This reaction is catalyzed by the hexokinase-II enzyme (HK-II) which is known to be overexpressed in tumor cells. The feeding of cancer cells can be prevented by inhibiting the hexokinase-II enzyme in the first step of aerobic glycolysis. In literature, Methyl Jasmonate (MJ) is known as a Hexokinase-II inhibitor since it disposes VDAC and HK-II interaction on mitochondrial membrane. In our study, we aimed to increase the activity by synthesizing the novel MJ analogues with appropriate modifications. Here we report Hexokinase-2 enzyme and cell viability study results in different cancer cells. Based on the three different cancer cell lines we investigated, our novel MJ analogues proved to be more potent than the original molecule. Thus this research may provide more efficacious/ novel HK-II inhibitors and may shed light to develop new anti-cancer agents.Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK

Biocompatibility of biomimetic multilayered alginate-chitosan/beta-TCP scaffold for osteochondral tissue

WOS: 000359166300047PubMed ID: 25982954Biomimetic three-layered monolithic scaffold (TLS) intended for treatment of osteochondral defects was fabricated by using freeze drying method. The multilayered material was prepared with chitosan (C) and alginate (A) polyelectrolyte complex (CA/PEC) as a cartilaginous layer, a combination of CA/PEC (60 wt%) and beta-tricalcium phosphate (beta-TCP) (40 wt%) as an intermediate layer and a combination of CA/PEC (30 wt%) and beta-TCP (70 wt%) as a subchondral layer in order to mimic the inherent gradient structure of healthy osteochondral tissue. Characterization of the scaffolds was performed using Fourier transform infrared (FT-IR) spectroscopy analysis, swelling and scanning electron microscopy (SEM) tests. In vitro cytotoxicity assay with L929 cells and EpiDerm skin irritation test (SIT) using the EpiDerm reconstructed human epidermal (RHE) model were performed to analyze biocompatibility of the scaffolds. Characterization results showed that there were strong ionic interactions among chitosan, alginate and beta-TCP and the layers showed interconnected porous structure with different swelling ratios. The relative cell viability and SIT results were greater than 70% indicating that the scaffolds are considered nontoxic according to the International Organization for Standardization (ISO) standard. All results taken together, biomimetic TLS can be considered to be suitable for osteochondral applications.Scientific Research Projects Coordination Unit of Istanbul University [50792]We sincerely thank Zehra Yilmaz and Ece Gurdal, Yeditepe University, Turkey. This work was supported by Scientific Research Projects Coordination Unit of Istanbul University. Project number: 50792