Gestasyonel diyabetin klinik periodontal durum ve plazma NT-ProBNP düzeylerinin değerlendirilmesi

Amaç: Çok az araştırmada, gestasyonel diabetes mellitus (GDM) ile enflamatuvar periodontal hastalıkların olası ilişkisi incelenmiştir. Bu çalışmanın amacı, GDM'si olan ve olmayan gebelerde klinik periodontal durum ve plazma NT-ProBNP düzeylerinin karşılaştırmalı olarak incelenmesidir. Gereç ve Yöntemler: Yükleme sonrası üç saat boyunca saatlik kan sekeri ölçümleri, açlık glukoz, açlık insülin ve HOMA-IR değerleri belirlenerek GDM tanısı konan 43 gebe ve GDM'si olmayan 41 gebe olmak üzere toplam 84 kadın çalışmaya dahil edildi. Plazma NT-ProBNP değerlendirmesi için venöz kan alınarak plazma örnekleri ayrıldı. Klinik periodontal durumun değerlendirilmesi için sondalama derinliği (mm), klinik ataşman seviyesi (mm), plak ve kanama indeksleri (%) ağızdaki tüm dişlerin 4 bölgesinde ölçüldü. Gebelere ait polikistik over, düşük hikayesi, insülin direnci, sosyo-demografik veriler, doğum haftası ve bebeklerin doğum ağırlığı kaydedildi. İstatistiksel analizler için MannWhitney U Testi ve Pearson ki-kare testi kullanıldı. Bulgular: GDM'si olan gebelerde yaş, açlık glukoz, açlık insülin, HOMA-IR, 1., 2. ve 3. saat glukoz değerlerinin GDM'si olmayan gebelere göre daha yüksek olduğu belirlendi (p < 0,05). Klinik periodontal veriler karşılaştırıldığında plak, kanama, ve sondalama derinliği ölçümleri her iki grupta benzer bulundu. Plazma NT-ProBNP seviyesi gruplar arasında anlamlı fark göstermedi. Sonuç: Çalışmamızın bulguları, GDM ile enflamatuvar periodontal hastalık şiddeti arasında anlamlı bir ilişki göstermemektedir. Ancak, konunun daha büyük çalışma gruplarında ve farklı biyokimyasal veriler aracılığıyla irdelenmesinin yararlı olacağını düşünüyoruz

Evaluation of gingival crevicular fluid cortisol levels with HPLC method [Dişeti olugu si{dotless}vi{dotless}si{dotless} kortizol düzeylerinin HPLC yöntemi ile degerlendirilmesi]

Objectives: The main aim of the present study was to evaluate the cortisol levels in gingival crevicular fluid by high performance liquid chromatography method. Secondary aim was to compare the cortisol levels in several periodontal diseases. Methods: Fifteen chronic periodontitis and 15 aggressive periodontitis patients were recruited. A total of 90 gingival crevicular fluid samples were collected from 3 teeth that had shallow, moderate and deep probing pocket depth of each patient. Gingival crevicular fluid samples were analyzed by high performance liquid chromatography method. Results: Gingival crevicular fluid samples of shallow pockets had lower volume (0.05µL>) than deep pockets (1-0.5 µL). Gingival crevicular fluid cortisol levels of shallow pockets were under the limit of detection. On the other hand, some of the gingival crevicular fluid cortisol levels of moderate and deep pocket samples were under the limit of detection. In this respect, it is hard to analyze and compare the cortisol levels of gingival crevicular fluid samples. Conclusions: Within the limitations of this study, it may be suggested that high performance liquid chromatography method is not an acceptable method to evaluate gingival crevicular fluid samples and to compare the cortisol levels in several periodontal diseases. © TurkJBiochem.com

Evaluation of Gingival Crevicular Fluid Cortisol Levels with HPLC Method

Objectives: The main aim of the present study was to evaluate the cortisol levels in gingival crevicular fluid by high performance liquid chromatography method. Secondary aim was to compare the cortisol levels in several periodontal diseases

Are monocyte chemoattractant protein-1-2518 G and regulated activation normal T cell expressed and secreted -403 A alleles risk factor for periodontitis?

Objective: We aimed to analyze the monocyte chemoattractant protein-1 (MCP-1) and regulated on activation, normal T cell expressed and secreted (RANTES) polymorphisms in relation to susceptibility to chronic periodontitis and influence of genotypes in response to non-surgical periodontal therapy. Methods: This cross-sectional clinical study was performed during 2005-2006 in Turkey. Gene polymorphisms were investigated in 51 periodontitis patients and 48 periodontally healthy subjects. Quadrant scaling and root planning was performed to periodontitis patients. Genomic DNA was analyzed for polymorphisms by polymerase chain reaction amplification followed by restriction enzyme digestion. Results: Genotype distributions of related genes were similar between periodontitis and healthy subjects (P>0.05). In periodontitis group, monocyte chemoattractant protein-1 -2518G allele frequencies were 25.5%, regulated on activation, normal T cell expressed and secreted-403A allele frequencies were 30.4%. Percentage of bleeding sites were significantly higher in regulated on activation, normal T cell expressed and secreted gene A negative genotypes compared to A positive (P=0.023). Significant improvements in clinical parameters were found after periodontal therapy in both genotypes (P<0.05). Conclusion: Within the limits of present data, results may suggest that there were no association between specific alleles and periodontal treatment effect. Furthermore, these gene polymorphisms are not associated with chronic periodontitis in Turkish population. © TurkJBiochem.com