Serum asymmetric dimethylarginine and nitric oxide levels in obese postmenopausal women

İstanbul Bilim Üniversitesi, Tıp Fakültesi.BACKGROUND: It has been reported that estrogen deficiency after menopause might cause a decrement in nitric oxide (NO) bioavailability by increasing the level of asymmetric dimethylarginine (ADMA), a major endogenous nitric oxide synthase inhibitor, thus leading to abnormalities in endothelial function. Because NO plays an important role on feeding behavior, ADMA may be involved in the pathogenesis of obesity, too. This cross-sectional study aimed to evaluate the relations of ADMA and NO with the obesity-linked peptides, such as ghrelin, leptin, and adiponectin in postmenopausal women free of hormone replacement therapy. METHODS: Adiponectin, ghrelin, leptin, ADMA, and NO(x) (total nitrite/nitrate) were measured in 22 obese (BMI: 30-47 kg/m(2)) and 19 normal weight (BMI: 21.5-26 kg/m(2)) postmenopausal women.Anthropometric measurements (height, weight, BMI, waist, and hip circumferences) were recorded. Statistics were made by the Mann-Whitney U-test. RESULTS: Ghrelin and adiponectin levels were significantly lower (P<0.001), whereas ADMA and leptin levels were higher in obese women than in normal weight controls (P<0.01 and 0.001, respectively). BMI was correlated negatively with adiponectin and ghrelin and positively with ADMA and leptin levels. No correlation existed between ADMA and NO. CONCLUSION: Estrogen deficiency alone may not cause an increase in ADMA levels unless the women are prone to disturbances in energy homeostasis. In spite of the high ADMA levels, the unaltered NO levels in plasma may be owing to ongoing inflammatory conditions

Curcumin uygulamasının yüksek yağlı diyetle beslenen sıçanlarda karaciğer yağlanması ve oksidatif stres üzerine etkisi

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Amaç: Sağlıkta kalite standartlarında bulunan çalışan güvenliği standartları çerçevesinde Biyokimya laboratuvarlarındaki potansiyel tehlikeler için farkındalığın artırılması ve kalite standartlarına uyumun sağlanması amacıyla risk analizi ve önleme planlarının tartışılması amaçlandı

Deneysel obezite niodelinde oral curcumin uygulamasının karaciğer trigliserid düzeyleri ve oksidatif stres üzerine etkisi

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Amaç: Gelişmiş toplumlarda görülme sıklığı artan obezite, metabolik hastalıklara yol açabilen sağlık sorunlarından biridir. Amacımız, yağlı diyetle beslenen sıçanlarda,.köri baharatının bir bileşeni ve tropikal bir bitki ekstresi olan curcuminin; kilo artışı, karaciğer yağlanması ve oksidatif stres üzerine etkisini araştırmaktır

The effect of artichoke leaf extract on hepatic triglyceride levels and oxidative stress in mice fed on high-fat diet

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Alkole bağlı olmayan yağlı karaciğer hastalığı (NAFLD) yaygın sağlık problemlerinden biridir. Antioksidan içeriği yüksek olan enginarın yapraklarından elde edilen ekstrenin (EYE) karaciğeri koruyucu etkisi olduğu bilinmektedir. Çalışmamızın amacı yüksek yağlı diyetle (YYD) beslenen farelerde karaciğer yağlanması ve oksidan-antioksidan denge üzerine EYE’nin etkilerini incelemektir

Effects of curcumin supplementation on liver metabolism in rats fed a high-fat diet: A proteomic approach

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Background: In the modern world, many individuals routinely consume diets rich in animal fat, leading to a high incidence ofobesity. Curcumin (diferuloylmethane, turmeric) extracted from the dried root of the rhizome Curcuma longa, is a popular Asian dietary spice used in curry. Curcumin is the most active component of turmeric, comprising 2-8% by weight. Proteomics is an important tool for elucidating the cellular response to various treatments including dietary factors

Urinary nerve growth factor in children with overactive bladder: A promising, noninvasive and objective biomarker

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Objective: This prospective study was designed to determine urinary nerve growth factor (NGF) levels in children with overactive bladder (OAB), and to evaluate whether this factor can be used as a biomarker for diagnosis and monitoring treatment outcome. Patients and methods: Urinary NGF levels were determined in 40 children with OAB and in a control group of 20 children with no urinary symptoms. Urine samples were collected from the patients prior to and at 3 and 6 months after the beginning of treatment. The total NGF levels (pg/mL) were further normalized to the concentration of urinary creatinine (NGF/Cr level). Results: Overall, both NGF and NGF/Cr levels were significantly higher at the beginning of the study. Mean NGF levels were 30.75 +/- 8.35 and 9.75 +/- 2.11 pg/ml (p=0.023) and mean NGF/Cr levels were 0.53 +/- 0.14 and 0.16 +/- 0.04 (p = 0.022) in patients and controls, respectively. After 6 months of therapy, the NGF/Cr level was significantly reduced to almost control levels (0.16 +/- 0.02, p = 0.047). Conclusion: NGF and NGF/Cr levels were significantly higher in children with OAB than controls at initial evaluation. Furthermore, the NGF/Cr level was significantly reduced following 6 months of therapy. NGF and NGF/Cr levels show promise as reliable biomarkers for OAB diagnosis and to monitor therapy in the pediatric age grou

Yüksek yağlı diyetle beslenen sıçanlarda testis dokusunda curcuminin oksidan-antioksidan sistem üzerine etkisi

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Yağlı beslenme alışkanlığı sonucu artan inflamasyon başta karaciğer olmak üzere pek çok organı etkilemektedir. Testis dokusunun reaktif oksijen ara maddelerine karşı duyarlı olduğu ve testiküler membranların çok doymamış yağ asitlerinden zengin olmasından dolayı mitokondriyal lipid peroksidasyosnunun bu dokuda arttığı daha önceki çalışmalarda gösterilmiştir. Curcumin (dferuloilmetan) bir baharat olan zerdaçalın bileşiminde bulunan, lipid peroxidasyonunu azalttığı ve hücresel antioksidan enzimleri uyardığı gösterilmiştir. Curcuma Longa bitkisinin bir birleşenidir. Bu çalışmanın amacı, curcuminin testis dokusunda yağlı beslenmeye bağlı gelişebilen lipid peroksidasyonu ve antioksidan enzim aktiviteleri üzerine olan etkisini incelemektedir

Effects of curcumin intake on fatty liver in high fat diet fed rats

İstanbul Bilim Üniversitesi, Tıp Fakültesi.Background: Long term high fat diet (HFD) intake causes lots of metabolic disorders and a major risk factor for nonalcoholic fatty liver. Curcumin (Cur) is a well-known compound of traditional turmeric "Curcuma longa" which has been shown to display antioxidative, anticarcinogenic, anti-inflammatory and hypocholesterolemic effects, The aim of this study was to evaluate the effects of curcumin treatment on liver fat accumulation and prooxidant-antioxidant status in an experimental high-fat diet fed rat model

The effect of dietary curcumin and capsaicin on hepatic fetuin-A expression and fat accumulation in rats fed on a high-fat diet

WOS: 000373476600008PubMed ID: 26706937Effects of curcumin (turmeric) and capsaicin (red pepper) on hepatic fat accumulation and fetuin-A expression in rats fed high-fat diet (HFD) is aimed to be investigated. Male Sprague-Dawley rats received HFD (60% of total calories from fat) and 0.15g capsaicin/kg HFD and/or 1.5g curcumin/kg HFD for 16 weeks. Hepatic AMPK, p-AMPK and fetuin-A expressions were determined by western blotting, liver lipid levels were measured with colorimetric methods and serum fetuin-A, insulin, leptin and adiponectin levels were detected using commercial ELISA kits. HFD increased hepatic lipid levels, fetuin-A expression and serum leptin, insulin and fetuin-A levels. Curcumin and capsaicin treatments significantly reduced hepatic fat accumulation and leptin levels; liver fetuin-A expression was decreased significantly by the curcumin treatment. Curcumin and capsaicin treatments attenuated hepatic fat accumulation and increased leptin levels related to inflammation. The suppression of hepatic fetuin-A expression is observed to be especially sensitive to curcumin.Istanbul University [39642]This work was supported by the Research Fund of Istanbul University. Project Number: 39642