Human sperm cryopreservation effects of sperm motility and dna fragmentation; to investigate relationship of this with the effect of leptin molecule

Radyoterapi, kemoterapi ve bazı maligniteler sonucu ejekülatuar disfonksiyon ve testiküler yetmezlik oluşabilir. Bu gibi durumlar için çiftlere çocuk sahibi olma konusunda yardımcı olabilecek metod sperm kriyoprezervasyonudur. Kriyoprezervasyon; hücrelerin ve dokuların sıfır derecenin altındaki sıcaklığa kadar soğutularak, bütün biyolojik aktivitelerinin durdurulması ve gelecekte kullanılması amacıyla saklanmasını ifade eder. Leptin molekülü; yenilenme, üreme, anjiyogenez, enerji harcanması, nöroendokrin sistemlere ilişkin düzenleme tokluk da dahil çoğu biyolojik süreçte rol oynamaktadır ve leptinin çeşitli çalışmalarda spermatogenez ile ilişkili olduğu belirtilmiştir. Bu çalışma ile kriyoprezervasyon öncesi ve sonrası parametrelerde leptin molekülünün sperm motilitesi ve DNA fragmantasyonu parametrelerinde bir marker (belirleyici) olarak kullanılabilmesi amaçlanmaktadır. Çalışmada Dünya Sağlık Örgütü (DSÖ) laboratuvar kılavuzuna göre normozoospermik olan 30 insandan alınan semen örnekleri kullanıldı. Kriyoprezervasyon öncesi ve sonrası; sperm motilitesi DSÖ kriterlerine göre, morfolojik sperm analizi Spermac Stain boyaması ile, DNA fragmantasyon analizi TUNEL ile, spermatozoa ultrastrüktüel yapı incelemesi geçirimli elektron mikroskobu ile, semen leptin seviyesi ölçümü ELISA yöntemi ile, ROT (Reaktif Oksijen Türleri) seviyeleri ise kalorimetrik yöntemlerle analiz edildi. Kriyoprezervasyon sonrasında öncesine oranla, sperm motilitesinde düşüş, morfolojik yapıda defektler, DNA fragmantasyonunda ise artış olduğu gözlemlenmiştir. Aynı şekilde semen ROT ve leptin seviyelerinde anlamlı ölçüde artış olduğu bulunmuştur. Bu çalışmamızın sonuçlarına göre; kriyoprezervasyon öncesi ve sonrası, normozoospermik hasta semen örneklerinde sperm motilitesi, DNA fragmantasyonu gibi parametrelerde, leptin molekülünün bir marker olarak kullanılabileceğini ve bu çalışmanın sonuçlarının klinik yönden erkek (in)fertilitesi üzerine yapılacak çalışmalara katkı sağlayacağını düşünmekteyiz.Human Sperm Cryopreservatıon Effects Of Sperm Motılıty And Dna Fragmentatıon; To Investıgate Relatıonshıp Of Thıs Wıth The Effect Of Leptın Molecule Radiotherapy, chemotherapy, some malignancies and even invasive surgery can result in ejaculatory dysfunction and testicular insufficiency. For a couples who are about to have a child can help stop the only proven method of sperm cryopreservation. Cryopreservation: accounts for, freezing the cells and tissues to below zero, stopping all the biological activities and keeping them for future use. Cryopreservation can cause some harmful changes in the structure and function of the sperm. Leptin molecule; plays a lot of roles in most biological processes including the satiety and renewal, proliferation, angiogenesis, energy expenditure, regulation of the neuroendocrine system and leptin was reported to be assosiated with spermatogenesis in several studies. This study aims leptin molecule to be used as a marker for sperm motility and DNA fragmentation parameters before and after the cryopreservation. In this study, according to the world health organization laboratory guide semen samples were taken from 30 normozoospermia group is used. Each semen sample is examined for the same paremeters before and after a cryopresevation process. Cryopreservation after and before; according to the sperm motility is going to be analysis based on WHO laboratory criteria, morphological sperm analysis with spermac stain dye, DNA fragmentation analysis by TUNEL, spermatozoa ultrastructure analysis with transmission electron microscopy(TEM), seminal leptin levels is measured with ELISA method and ROS levels is analysed by the calorimetric method. After cryopreservation than before, there is a decrease in sperm motility, distribution of sperm morphology and there is an increase in DNA fragmentation. Similarly, semen in ROT levels are also found to be increased significantly. According to results of this study; after and before cryopreservation, in normozoospermia patient semen samples in parameters sperm motility and such as DNA fragmentation; leptin molecule can be used as a marker and it can contribute to further studies on man (in)fertility

The relationship of seminal leptin levels with semen parameters and dna fragmentation

Amaç: Bu çalışmamızda insan semen leptin düzeylerini ve semen parametreleri ile ilişkisinin incelenmesi amaçlanmıştır. Materyal ve Metod: YNormal ve oligoastenoteratospermik 30 hastalık gruplar oluşturuldu. Hastaların semen örneklerinde leptin ve DNA fragmantasyon düzeyleri incelendi, ayrıca sperm sayısını ve motilite oranlarını içeren semen parametreleri de- ğerlendirildi. Bulgular: İnfertil hastalarda sperm sayısı ve motilite oranları normal gruba göre anlamlı olarak azalmıştır: 6,40±4,29 (x106/ mL), % 38,63±14,92 (p<0,0001). DNA fragmantasyon oranları normal grupta % 21,00±8,23 olarak gözlenirken infertil erkeklerde % 38,43±14,23 oranıyla anlamlı olarak yüksek bulunmuş, semen leptin oraları ise normal grupta 347,58±111,13 ng/mL olarak gözlenirken, infertil grupta 267,63±56,36 ng/mL düzeyi ile anlamlı olarak azaldığı gözlenmiştir. İnfertil gruplar kendi içinde incelendiği zaman ise semen leptin düzeyi ile yaş, volüm, sperm sayısı ve motilitesi arasında istatistiksel olarak anlamlı olacak bir ilişki saptanmamıştır. Sonuç: Çalışmamızda, oligoastenoteratozoospermik erkek infertilitesinde serum leptin seviyelerinin azalmış olduğunu, ama sperm motilitesi ile anlamlı bir ilikinin bulunmadı gözlemlenmiştir. Leptinin semende tanısal bir kriter olarak kullanılabilmesi için daha geni kapsamlı çalışmaların yapılması gerektiği düşünülmektedir.Objective: The objective of this study is to investigate the relationship between human seminal leptin levels and semen parameters.Material and Methods: Study groups include 30 males with oligoasthenoteratozoospermia and 30 males who have normal semen parameters. Leptin and DNA fragmentation levels were investigated in both groups, along with a variety of semen parameters including sperm count and motility.Results: In comparison with the normal males, sperm count and motility have decreased significantly in infertile patients: 6,40&plusmn;4,29(x106/mL), %, 38,63&plusmn;14,92 (p&lt;0,0001). DNA fragmentation levels were found as 21,00&plusmn;8,23 % and 38,43&plusmn;14,23 %, in normal and patient groups respectively. Seminal leptin levels were 347,58&plusmn;111,13 ng/mL in the normal group, while there was a significant decrease to 267,63&plusmn;56,36 ng/mL in the infertile group. Among infertile patient groups, no significant difference was observed regarding the relationship between seminal leptin levels and age, volume, sperm count and motility.Conclusion: In our study it was demonstrated that, serum leptin levels decrease in oligoasthenoteratozoospermic infertile males, while there is not a significant relation with the motility of sperm. In order to use seminal leptin levels as a diagnostic tool more extensive studies are needed

Leptin in sperm analysis can be a new indicator

WOS: 000461000600006PubMed ID: 30482507Purpose Radiotherapy, chemotherapy, various tumors and invasive surgery can result in ejaculatory dysfunction and testicular insufficiency. Sperm cryopreservation is the only method which can provide a baby for couples. Cryopreservation freezes tissues and cells, allowing them to be stored for future use by stopping all biological activities. Cryopreservation can cause some harmful changes in the structure and function of the sperm. Leptin molecule plays many roles in most biological processes including the satiety and cell renewal, proliferation, angiogenesis, modulation of energy expenditure and regulation of the neuroendocrine system. Leptin was also reported to be associated with spermatogenesis in several studies. Methods This study aims to use leptin molecule as a parameter for sperm motility and DNA fragmentation before and after the cryopreservation. In this study, semen samples were taken from 30 normospermic male volunteers. Each semen sample was examined for the same parameters before and after the cryopreservation. Samples were analyzed before and after cryopreservation in terms of sperm motility by morphological sperm analysis with spermac stain dye, DNA fragmentation by TUNEL assay, ultrastructural analysis with transmission electron microscopy (TEM), seminal leptin levels by ELISA method and reactive oxygen species (ROS) levels by colorimetric method. Results Decreased sperm motility, distribution of sperm morphology and increased DNA fragmentation were determined after cryopreservation. Similarly, seminal ROS and leptin levels were also increased significantly. There was a negative correlation between seminal leptin and sperm motility. Additionally, there was a positive correlation between seminal leptin and DNA fragmentation. Conclusion According to these results, leptin molecule can be used as a marker for sperm motility and DNA fragmentation before and after cryopreservation. We think that the results of this study can contribute to further studies in the clinical aspect.Research Fund of Medipol University-Istanbul/Turkey [BAP 86770134-604/13]This study was supported by the Research Fund of Medipol University (BAP 86770134-604/13)-Istanbul/Turkey as Msc. thesis. We thank Dr. M. Serif Aydin for contributions in the laboratory

Research Data for Is SARS-CoV-2 viral load a predictor of mortality in COVID-19 acute respiratory distress syndrome patients?

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