Hsa-miR-584-5p as a novel candidate biomarker in Turkish men with severe coronary artery disease

Coronary artery disease (CAD) is still the preliminary cause of mortality and morbidity in the developed world. Identification of novel predictive and therapeutic biomarkers is crucial for accurate diagnosis, prognosis and treatment of the CAD. The aim of this study was to detect novel candidate miRNA biomarker that may be used in the management of CAD. We performed miRNA profiling in whole blood samples of angiographically confirmed Turkish men with CAD and non-CAD controls with insignificant coronary stenosis. Validation of microarray results was performed by qRT-PCR in a larger cohort of 62 samples. We subsequently assessed the diagnostic value of the miRNA and correlations of miRNA with clinical parameters. miRNA-target identification and network analyses were conducted by Ingenuity Pathway Analysis (IPA) software. Hsa-miR-584-5p was one of the top significantly dysregulated miRNA observed in miRNA microarray. Men-specific down-regulation (p = 0.040) of hsa-miR-584-5p was confirmed by qRT-PCR. ROC curve analysis highlighted the potential diagnostic value of hsa-miR-584-5p with a power area under the curve (AUC) of 0.714 and 0.643 in men and in total sample, respectively. The expression levels of hsa-miR-584-5p showed inverse correlation with stenosis and Gensini scores. IPA revealed CDH13 as the only CAD related predicted target for the miRNA with biological evidence of its involvement in CAD. This study suggests that hsa-miR-584-5p, known to be tumor suppressor miRNA, as a candidate biomarker for CAD and highlighted its putative role in the CAD pathogenesis. The validation of results in larger samples incorporating functional studies warrant further research

Coronary artery disease related Mir-19A and Mir-26A are sensitive to simvastatin and ror-alpha ligands in macrophage cells

87th Congress of the European-Atherosclerosis-Society (EAS)European Atherosclerosis So

Okul Öncesi Öğretmenlerinin Medyanın Çocukların Beslenme Alışkanlıkları ve Bozuklukları Üzerindeki Etkisine Yönelik Bakış Açıları

Son yıllarda medyanın bireyin yaşantısındaki yansımaları göz önüne alındığında zararlı beslenme alışkanlıkları ve bozuklukları bunlardan sadece birkaçıdır. Medyanın çocukların beslenme alışkanlıkları ve bozuklukları üzerindeki etkisini okul öncesi öğretmenlerinin gözüyle değerlendirmek amacıyla yapılan bu çalışmada nitel araştırma yöntemi kullanılmıştır. Veriler, Ankara ilinde görev yapan 12 okul öncesi öğretmeni ile yapılan yarı yapılandırılmış görüşme tekniği ile elde edilmiştir. İçerik analizi tekniği ile analiz edilen verilere göre çalışmada 7 adet tema bulunmaktadır. Yapılan analiz sonucunda okul öncesi öğretmenlerinin, ailenin yeme konusundaki tutumunun ve medyanın çocuklardaki beslenme alışkanlıkları ve bozuklukları üzerinde etkili olduğunu düşündükleri bulunmuştur. Elde edilen veriler ışığında, çocuklarda sağlıklı beslenme alışkanlıklarının oluşması için sağlıklı beslenme üzerine farkındalık sağlamayı amaçlayan projeler planlanması, ailelere medya okuryazarlık eğitimleri ve aile katılımını sağlayacak eğitimlerin düzenlenmesi gibi öneriler sunulmuştur. Anahtar Kelimeler: medya, çocuklar, beslenme alışkanlıkları, beslenme bozuklukları, okul öncesi öğretmenler

Cholesterol-related gene variants are associated with diabetes in coronary artery disease patients

Coronary artery disease (CAD) which is a complex cardiovascular disease is the leading cause of death worldwide. The changing prevalence of the disease in diferent ethnic groups pointing out the genetic background of CAD. In this study, we aimed to evaluate the contribution of selected cholesterol metabolism-related gene polymorphisms to CAD presence. A total of 493 individuals who underwent coronary angiography were divided into 2 groups: normal coronary arteries (?30% stenosis) and critical disease (?50% stenosis). Individuals were genotyped for APOC1 (rs11568822), APOD (rs1568565), LIPA (rs13500), SORL1 (rs2282649), and LDLR (rs5930) polymorphisms using hydrolysis probes in Real-Time PCR. Blood samples were drawn before coronary angiography and biochemical analyses were done. The results were statistically evalu ated. When the study group was stratifed according to CAD, the minor allele of APOD polymorphism was found related to decreased risk for T2DM in the non-CAD group. In logistic regression analysis adjusted for several confounders, LDLRrs5930 polymorphism was found associated with T2DM presence in the male CAD group [OR=0.502, 95%CI (0.259–0.974), p=0.042]. Besides, APOD and LIPA polymorphisms were shown to afect serum lipid levels in non-CAD T2DM patients (p<0.05). The minor allele of APOC1 was found associated with triglyceride levels in males independent of CAD status. Besides, LDLR minor allele carrier females had elevated HbA1c and glucose levels independent from CAD status in the whole group. The cholesterol metabolism-related gene polymorphisms were found associated with T2DM and biochemical parameters stratifed to sex, CAD, and T2DM status

The First Record of Raccoon Dog (Nyctereutes Procyonoides) in Turkey

The raccoon dog (Nyctereutes procyonoides) was recorded for the first time in Turkey on May 10, 2019, and June 5, 2019, in the same location after 4668 nights of camera trapping in the forests of the Sarıkamış region and Allahuekber Mountains in eastern Turkey. It was recorded in a Scots pine (Pinus sylvestris) forest at 2340 m above sea level with extensive snow cover. Given that the nearest known population is in the forests of Georgia approximately 105 km away, there may already be a viable population in Turkey. As an omnivorous species with a high adaptive ability and high reproductive potential, the raccoon dog has strong dispersal capability. It is considered an invasive species in Europe and there are some ongoing eradication activities in several countries. Therefore, we strongly advise that the raccoon dog in Turkey be evaluated as an invasive species and that the relevant authorities conduct extensive research and any necessary management, especially where the habitat and local climate are more favorable for the species’ reproduction and range expansion

The role of lipoprotein metabolism and inflammatory gene polymorphims on the coronary artery disease with type 2 diabetes mellitus

86th Congress of the European-Atherosclerosis-Society (EAS) -- MAY 05-08, 2018 -- Lisbon, PORTUGALEuropean Atherosclerosis So

The role of lipid, sex hormone and inflammation pathway genes polymorphims on coronary artery disease in turks

86th Congress of the European-Atherosclerosis-Society (EAS) -- MAY 05-08, 2018 -- Lisbon, PORTUGALEuropean Atherosclerosis Societ