Identification of salt stress responsive protyeins in wild sugar beet (Beta maritima) using 2D-page with MALDI-TOF/TOF system

Thesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2012Includes bibliographical references (leaves: 30-34)Text in English; Abstract: Turkish and Englishxiii, 55 leavesHigh salinity is one of the abiotic stresses, which affects the homeostasis, growth and productivity of plants. In plants, uptake of the non-essential salt ions negatively affects the anatomy, physiology and metabolism, changes the osmotic balance in cells and causes abundant dehydration. In this case, higher plants develop salt tolerance mechanisms such as induction of related signaling pathways, effluxion of salt ions, accumulation of these toxic ions in their vacuoles, activation of their detoxification mechanisms and production of osmoprotectans. In this study, identification of salt responsive proteins in moderately halophyte wild type sugar beet Beta vulgaris ssp. maritima was aimed. In order to investigate the protein-based natural stress tolerating mechanisms, plants were exposed to 150 mM NaCl and total proteins were extracted. Differentially expressed proteins were identified by proteomic approaches including MALDI-TOF/TOF mass spectrometry combined two dimensional polyacrylamide gel electrophoresis. The results revealed that enzymatic antioxidants and secondary members of antioxidative pathways are responsive in salt stress. In conclusion, these detected proteins demonstrate that antioxidative system may be the major defense mechanism in halophytic plants

Identification of salt stress responsive protyeins in wild sugar beet (Beta maritima) using 2D-page with MALDI-TOF/TOF system

Thesis (Master)--Izmir Institute of Technology, Molecular Biology and Genetics, Izmir, 2012Includes bibliographical references (leaves: 30-34)Text in English; Abstract: Turkish and Englishxiii, 55 leavesHigh salinity is one of the abiotic stresses, which affects the homeostasis, growth and productivity of plants. In plants, uptake of the non-essential salt ions negatively affects the anatomy, physiology and metabolism, changes the osmotic balance in cells and causes abundant dehydration. In this case, higher plants develop salt tolerance mechanisms such as induction of related signaling pathways, effluxion of salt ions, accumulation of these toxic ions in their vacuoles, activation of their detoxification mechanisms and production of osmoprotectans. In this study, identification of salt responsive proteins in moderately halophyte wild type sugar beet Beta vulgaris ssp. maritima was aimed. In order to investigate the protein-based natural stress tolerating mechanisms, plants were exposed to 150 mM NaCl and total proteins were extracted. Differentially expressed proteins were identified by proteomic approaches including MALDI-TOF/TOF mass spectrometry combined two dimensional polyacrylamide gel electrophoresis. The results revealed that enzymatic antioxidants and secondary members of antioxidative pathways are responsive in salt stress. In conclusion, these detected proteins demonstrate that antioxidative system may be the major defense mechanism in halophytic plants

Eğitim fakültesi öğrencilerinin bilgiye erişim biçimleri üzerine bir tarama çalışması

Bu projede eğitim fakültesi öğrencilerinin bilgiye erişim biçimlerini ve alışkanlıklarını belirlemek üzere bir tarama yapılacaktır. Veriler anket yoluyla toplanacaktır. Araştırmada çalışma grubunu eğitim fakültesi öğrencileri oluşturacaktır

A sixth-grade student's growth in understanding written proof texts: Pre-and post-interview analyses of a teaching experiment study

International audienceThis study aims to describe a 6 th-grade student's progress in understanding written proof texts through participating in an individual teaching experiment. The same task was administered to the student twice in an interview setting, before and a year after the teaching experiment. The student was asked to evaluate four arguments aimed to prove a given conjecture. While in the pre-interview, the student accepted all four arguments based on her Naïve Experience; in the post-interview, she rejected empirical arguments and looked for General Procedures (or Abstract Structures) that necessarily apply to the whole set of numbers under investigation. In the post-interview, spontaneous changes occurred in the student's understanding of the given arguments after she constructed her own proof for the same conjecture. Instructional design elements used in the teaching experiment might have facilitated her understanding of the structure of deductive proof texts

Ortaokul Matematik Öğretmen Adaylarının Matematiksel Tanım Ve Tanımın Matematik Eğitimindeki Önemine Yönelik Algılarının İncelenmesi

Bu çalışmanın amacı dördüncü sınıf ortaokul matematik öğretmeni adaylarının matematiksel tanım algılarını birbirini tamamlayan iki farklı şekilde incelemektir. Öncelikle öğretmen adaylarına matematiksel tanım anlayışlarını yansıtabilecekleri bir görev iki farklı şekilde verilecektir. Bu görevde öğretmen adaylarından kendilerine alfabetik sırada verilen dörtgenleri (deltoid, dikdörtgen, eşkenar dörtgen, kare, paralelkenar, yamuk) herhangi bir öğretim programına bağlı kalmadan tercih ettikleri sıra içerisinde tanımlayarak ikinci bir kişiye tanıtmak üzere bir dizilim oluşturmaları istenecektir. Bu süreç ilk kez uygulandığında katılımcılara sadece dörtgenlerin isimleri verilecek, ve onlardan kendi tanımlarını yapmaları istenecektir. Süreç ikinci kez uygulanırken ise adaylara her bir dörtgen için birden fazla birbirine eş tanım ifadesi verilecektir, katılımcılar istedikleri tanımları seçip kullanabileceklerdir. Ardından, öğretmen adaylarının tanımın matematik disiplinindeki yeri ve önemine dair görüşleri açık uçlu sorulara verdikleri yazılı yanıtlar doğrultusunuda incelenecektir. Öğretmen adaylarının matematik eğitiminde tanımın yeri ve önemi hakkındaki görüşleri ise ayrıca incelenecektir. Çalışmadan elde edilen bulgular bütüncül bir şekilde yorumlanacaktır

Proceedings of the Eleventh Congress of the European Society for Research in Mathematics Education

The research reported here is part of an ongoing study in which prospective middle school mathematics teachers’ conceptions of definition are investigated through their responses to semi-structured interview questions about defining quadrilaterals. Here we present findings from their responses to a subset of the interview questions, with the purpose of understanding what they mean by the expression “definitions can be proved”- an expression commonly referenced, and considered as erroneous in the research literature. Analysis of the responses, through using thematic coding and Toulmin’s (1958) scheme, revealed that participants attributed two different meanings to the phrase: (1) proving the claim that a written definition accurately designates an intended concept and (2) proving the concept being defined (erroneous). Based on our findings, we point to a reconsideration of the phenomenon by the research community.                    </p

Au cœur de l'État At the heart of the State

La conférence internationale « Au Cœur de l’État. Comment les institutions traitent leur public » se déroule à l’École des Hautes Études en Sciences Sociales les 11 et 12 juin. Soutenu par l’Advanced Grant du Conseil européen de la recherche dont Didier Fassin est lauréat, ce colloque, dont l’Institute for Advanced Study de Princeton est partenaire, présente et discute des travaux conduits au sein de l’Iris, Institut de recherche interdisciplinaire sur les enjeux sociaux (CNRS-Inserm-EHESS-UP13)

Characterization of the BETA1 gene, which might play a role in Beta vulgaris subsp. Maritima salt tolerance

Salinity stress has a negative impact on plant growth, which affects homeostasis and productivity. The uptake of nonessential salt ions changes the osmotic balance of the cell and causes dehydration. Higher plants develop salt tolerance mechanisms to avoid dehydration. Sea beet (Beta vulgaris subsp. maritima) is a halophytic ancestor of cultivated sugar beet that displays salt stress tolerance. In this study, we screened a B. vulgaris subsp. maritima cDNA library in Saccharomyces cerevisiae strain Ab11c (ena1Δ, nha1/4Δ, nhx1Δ), which is deficient in sodium transport, to find sodium-detoxifying genes. We identified a cDNA construct, named BETA1, providing salt tolerance to yeast cells. This gene had no previously described function. Intracellular sodium measurements demonstrated no significant differences between yeast cells expressing BETA1 or a sham vector, suggesting that sodium was not effluxed in BETA1-expressing cells. Transcriptionally, BETA1 mRNA levels were induced immediately in leaves and later in the root system in response to the salt stress. Our results suggest that the BETA1 gene is part of the salt tolerance network in B. vulgaris subsp. maritima.Scientific and Technological Research Council of Turkey (115Z694