The evaluation of serum pancreatic derived factor (PANDER) and malondealdehyde (MDA) levels in women with polycystic ovarian syndrome

Polikistik Over Sendromu (PKOS) insülin direnci, dislipidemi, sistemik inflamasyon ve endotel disfonksiyonu gibi metabolik bozukluklarla birliktelik gösterir. Polikistik over sendromlu hastalarda gelişebilen DM etiyolojisine yönelik oksidatif stresin ve pankreas hücrelerinde meydana gelebilecek hasarlanmanın rolünü araştırdık. Pankreas hücrelerindeki yıkımı belirlemek için Pancreatic Derived Factor (PANDER veya FAM3B) ve oksidatif stres parametresi olarak ta malondialdehit (MDA) kullanıldı. Yaş aralığı 18-38 olan 27 PKOS?lu hasta çalışma grubu olarak, 24 sağlıklı kadın ise kontrol grubu olarak çalışmaya alındı. Demografik verileri, antropometrik ölçümleri, hormonal ve metabolik pro?lleri, serum PANDER ve MDA düzeyleri değerlendirildi. Çalışmamızda hasta ve kontrol grubu arasında serum MDA ve PANDER değerleri arasında anlamlı bir farklılık bulunmamıştır. PKOS?lu grupta açlık kan şekeri, açlık insülin düzeyleri ve HOMA-IR düzeyleri kontrol grubuna göre anlamlı olarak yüksek saptandı HOMA-IR düzeyleri ile FAI (r = 0,50 p = 0,0001), HOMA-IR düzeyleri ile BMI (r = 0,55 p = 0,0001) ve HOMA-IR düzeyleri ile TG (r = 0,35 p = 0,013) arasında pozitif ilişki saptandı. Çalışmamızda PKOS?lu hastalarda anlamlı olarak artmış FAI ve serum total testosteron düzeyleri ile azalmış olan SHBG düzeyleri tespit edildi. Yaptığımız çalışmada Polikistik Over Sendromlu hastalarda gelişen DM un etiyolojisinde oksidatif stres etkisi ve insülin sekresyonu yapan hücrelerde hasarlanma olmadığı ortaya konmuştur. Daha doğru bir yorum için uzun süreli, randomize ve kontrollü klinik çalışmaların yanı sıra moleküler araştırmalara da ihtiyaç vardır. Anahtar kelimeler: Polikistik over sendromu, PKOS, Pander, MalondialdehitPolycystic ovary syndrome (PCOS) is associated with metabolic derangements including insulin resistance, dyslipidemia, systemic inflammation and endothelial dysfunction. In this study we investigated the role of oxidative stres and pancreatic cell damage as possible factors in development of DM in PCOS. Pancreatic Derived Factor (PANDER) and Malondealdehyde (MDA) were used as markers of pancreatic cell damage and oxidative stress, respectively. 27 patients with PCOS and 24 healthy women as control group ages of whom ranging between 18-38 were included in the study. Demographic data, antropometric measurements, hormonal and metabolic status, serum MDA and PANDER levels were evaluated. Serum MDA and PANDER levels did not differ significantly between the study and control groups. Significantly higher serum fasting glucose, insulin levels and HOMA-IR values were observed in women with PCOS in our study. Positive correlations were observed between HOMA-IR and FAI, BMI ,TG levels ((r = 0,50 p = 0,0001, r = 0,55 p = 0,0001, r = 0,35 p = 0,013, respectively). The patients with PCOS had considerably higher FAI and serum total testosterone leves than healthy subjects, while significantly lower serum SHBG concentrations were determined. Our study demonstrated that oxidative stress and pancreatic cell damage has no role in the etiology of DM in PCOS. However, in terms of PANDER activity, randomized, case-controlled, long-term studies are needed in PCOS, especially in molecular basis. Keywords: Polycystic ovary syndrome, PCOS, PANDER, malondialdehyd

Effect of ejaculatory abstinence period on sperm DNA fragmentation and pregnancy outcome of intrauterine insemination cycles: A prospective randomized study

Purpose: To evaluate the effect of the ejaculatory abstinence period on sperm DNA fragmentation and pregnancy rates in IUI cycles. Methods: One hundred and twenty couples with unexplained infertility were prospectively randomized into two groups on the second day of their cycle. In group A, patients had 1-day ejaculatory abstinence period; in group B patients had 3-day ejaculatory abstinence period. Each patient was stimulated with gonadotropins, and IUI was performed. The primary outcome measure was clinical pregnancy and sperm DNA fragmentation rate. Results: The pregnancy rate was 17.3% and 18.5% in group A and group B, respectively. No significant difference was found among the groups (p = 0.803). The sperm DNA fragmentation rate for group A (20.71 ± 11.01) and group B (23.78 ± 12.64) were almost similar (p = 0.187). The sperm DNA fragmentation rate was 24.89 ± 12.89 in pregnant couples and 21.71 ± 11.69 in non-pregnant couples (p = 0.288). Conclusion: Even after a short abstinence period of 1 day, compared to the standard abstinence period of 3 days, pregnancy rates are similar among unexplained infertility couples undergoing ovarian stimulation and IUI. ClinicalTrials: The study was registered at ClinicalTrials.gov with ID NCT04361292. Date of registration: 27 April 2020. The study was registered retrospectively. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature

Nonobstruktif azospermi olgusunda testisten mezenkimal kök hücrelerin izolasyonu

Azospermi semende hiç sperm bulunamaması durumudur. Obstrüktif ve nonobstrüktif olmak üzere iki çeşidi vardır. Testiste spermatojenik seriyi oluşturan sperm kök hücresinden (spermatogonium) başka mezenkimal kök hücre (mkh) ve VSELs (çok küçük embriyonik benzeri kök hücreler) de vardır. Mezenkimal kök hücreler stromal kökenli erişkin kök hücreleridir ve birçok dokuda bulunmaktadır. Mkh’lerin testiste germ hücrelerinin mikroçevresini oluşturduğu gösterilmiştir. Bizim bu çalışmamızdaki amacımız nonobstruktif azosperminin bir çeşidi olan sertoli cell only sendroma sahip bir olguda mezenkimal kök hücreleri izole etmek ve bu hücrelerin karekterizasyonunu incelemektir

Evaluation of sexual functions and quality of life in female patients after hysterectomy for benign symptomatic diseases

Objective: To evaluate selected quality of life parameters among sexually active patients who underwent a total abdominal hysterectomy and bilateral salpingo-oophorectomy (TAH-BSO) for benign symptomatic disease. Materials and Methods: Fifty-six TAH-BSO patients (20 menopausal + 36 premenopausalhysterectomized women) were evaluated between September 2017 and May 2018 using the FSFI, ASEX and SF-36 scores with individual interviews before and six months after TAH + BSO surgery. Results: The preoperative and postoperative period scores from three different validated questionnaires were found to be significantly different for all patients. Scores in the postoperative periods were better in all patients. Also, scores in the preoperative and postoperative periods were higher among premenopausal patients than menopausal patients. Conclusion: Our results showed that sexual function and quality of life improved in all patients who underwent TAH-BSO for benign symptomatic disease. Also, menopausal status is very important. ©2020 Koşar Can et al. Published by IMR Press

Serum Caspase-1 levels in women with polycystic ovary syndrome

Objective: Caspase-1 is implicated in several important inflammatory diseases and controls adipocyte differentiation and insulin sensitivity. Interleukin-10 (IL-10) is an anti-inflammatory cytokine and plays an important role in chronic inflammatory conditions. This study was planned to determine if there is any relationship between Caspase-1 and IL-10 levels in women with PCOS. Materials and methods: Forty-two women with PCOS and thirty-seven healthy controls were evaluated in this controlled clinical study. Caspase-1 and IL-10 levels, serum lipid sub-fractions, fasting glucose, fasting insulin and other hormones (gonadotropins, androgens), malondialdehyde (MDA) and glutathione (GSH) levels were measured. Homeostasis model assessment (HOMA-IR) was used to estimate insulin resistance. Results: Free androgen index (FAI), HOMA-IR, MDA and Caspase-1 levels were significantly higher in subjects with PCOS. However, the women with PCOS had considerably lower GSH concentration levels than healthy subjects. Serum IL-10 levels were higher in study subjects than in controls, though it was statistically insignificant. Caspase-1 was positively associated with IL-10. Conclusion: These outcomes propose that Caspase-1 may have a role in triggering the processes leading to chronic low-grade inflammation in women with PCOS, independent of insulin resistance, androgen excess and oxidative stress. Nevertheless, the precise role of Caspase-1 in the pathogenesis of the disease remains to be elucidated. © 202

Do seasonal variations in ambient temperature, humidity and daylight duration affect semen parameters? A retrospective analysis over eight years

We aimed to evaluate the possible effects of seasonal variation on semen parameters. We retrospectively analysed the data of 6,116 semen samples collected at a university hospital for eight years. The past ambient temperature, relative humidity and daylight duration records, and birth registry of the province were obtained to examine the relationship of seasonal changes in semen parameters with annual birth rates and environmental factors. The mean age was 33.03 ± 6.86 years. We found a significant difference between months for sperm concentration (p <.0001), total sperm count (p <.0001), progressively motile sperm count (p <.0001) and normal sperm morphology (p =.028). The sperm concentration and total count were significantly lower in July and August compared with December, May and June. The progressively motile sperm count in October was 23.6% less than the value of May. The temperature and temperature–humidity index were negatively correlated with semen parameters. The highest number of births was in the summer. However, no correlation was present between deliveries and the semen concentration regarding months (rs = 0.199, p =.083). In conclusion, we observed significant seasonal and monthly differences in sperm concentration, sperm count and progressively motile sperm count. Increased ambient temperature due to seasonal changes may be a detrimental factor for semen parameters. © 2020 Wiley-VCH Gmb

Polikistik over sendromlu kadınlarda serum omentin, osteoprotegerin ve malondialdehit düzeylerinin değerlendirilmesi: prospektif vaka kontrol çalışması

Purpose: The aim of this study was to evaluate the serum levels of omentin, osteoprotegerin and malondialdehyde levels in patients with polycystic ovary syndrome (PCOS).Material and Methods: This prospective case-control study included 31 patients with the diagnosis of PCOS and 33 healthy cases as a control group. Both groups were compared according to demographic characteristics, antropometric measurements, levels of biochemical parameters and serum levels of omentin, osteoprotegerin and malondialdehyde.Results: When compared with control group, patients in PCOS group were found to have statistically significantly lower age but have higher hirsutism score, fasting glucose level, total testosteron level, free androgen index, LH level and LH/FSH ratio. There was no statistically significant difference among PCOS and control groups in terms of serum omentin, osteoprotegerin and malondialdehyde levels.Conclusion: No significant change was shown in the levels of serum omentin, osteoprotegerin and malondealdeheyde levels in women with PCOS