Interplay between glucose and palmitate uptake in breast carcinoma in vitro

One of the most studied tumor cells lines in vitro is the breast carcinoma MDA-MB-231 cell line. Several studies have proved its glycolytic profile, namely known as the Warburg effect. Glutamine oxidation is also important for its metabolism. Nevertheless, the use of fatty acids for obtaining energy in these cells is still rising. Palmitic acid is the most common saturated fatty acid, containing sixteen carbons in its structure. However, the use of palmitate for metabolic studies in MDA-MB-231 is not very extended due to its pro-apoptotic effect in this cell line after certain time exposure. Nonetheless, in this work we used palmitate as a metabolic fuel for just 30 minutes in order to see the almost immediate response of the cells to its presence, after a 30 minutes fast period. Our results show that MDA-MB-231 cells are not able of oxidizing palmitate nor producing lactate from it. Simultaneous presence of palmitate with glucose or with glutamine does not affect glucose nor glutamine uptake in these cells. However, we observed that even low concentrations of glucose increase palmitate uptake in MDA-MB-231 after a 30 minutes incubation. Treatment with 5 mM 2-deoxyglucose also for 30 minutes counters this rise, since 2-deoxyglucose diminishes palmitate uptake. Increasing glucose concentration to the same dosis of 2-deoxyglucose leads to a prevalence of the glucose effect on palmitate uptake. The exact role of glucose and glucose derivatives should be further studied in order to know more about palmitate metabolism in this cell line.Our experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain). This communication has the support of a travel grant "Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech"

Fasentin, a glucose uptake inhibitor, is also able to inhibit angiogenesis

Es comunicación a congreso en formato pósterThe role of glucose on endothelial cell (EC) metabolism and angiogenesis has been an emerging issue in the last few years. Some inhibitors of glucose metabolism, such as 2-deoxyglucose, have been shown to have anti-angiogenic effects. Fasentin is a poor-studied inhibitor of glucose uptake which modulates GLUT-1 and GLUT-4 transporters in cancer cells. We wanted to test its possible effect on EC glucose uptake, showing a light decrease in HMEC at 100 µM. Lower doses did not affect this characteristic of glucose metabolism. In line with this fact, fasentin at 100 µM totally inhibited tube formation on Matrigel in these cells. This anti-angiogenic effect is not likely to be helped by a pro-apoptotic effect of fasentin but, as proved with additional assays, it could be due to a decrease on the signaling for extracellular matrix degradation. More research would be necessary in order to elucidate its fine regulation on angiogenesis and metabolism.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. [Our experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain). This communicaction has the support of a travel grant "Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech"]

Synthesis and biological activity of a new class of antitumor cyclopeptides based on the solomonamides

Solomonamides A (1) and B (2) are novel natural products recently isolated from the marine sponge Theonella swinhoei [1]. Preliminary structural studies revealed an unprecedented cyclic peptide type structure. Interestingly, solomonamide A exhibits anti-inflammatory activity, showing potent reduction (60%) of inflammation at a very low concentration of 100 µg/kg in animal models. However, the scarcity of these compounds from their natural sources has been a drawback for further pharmacological assays. In fact, the anti-inflammatory activity of solomonamide B was not evaluated due to the limited amounts. This difficulty to access large amounts of these compounds makes quite difficult to gain insight into their biological profiles and mechanism of action and justifies the chemical synthesis of this new class of cyclic peptides. As a consequence, the solomonamides have been the subject of several synthetic efforts [2] notably by the Reddy group who has recently reported the first total synthesis of solomonamide B based on a intramolecular Heck reaction, which led to a revision of the initially proposed structure for 2 [3].Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Our ten years of work on transparet box business simulation

Traditional business games are of the so-called black-box type (BBBS=Black box business simulator); that is to say, the internal structure which generates the results of the simulation after decision-making is not known. As a result, the player normally operates by trial and error and bases his decisions on the symptoms of the problem (the observed behaviors of the system's variables) and not on the real causes of the problem (the system's structure). Since 1988 José A.D. Machuca has insisted that the business games based on System Dynamics models should be Transparent-box business simulators (TBBSs). That means that, during the game, the user has access to the structure of the underlying model and is able to relate it to the observed behaviors. The hypothesis is that such transparency would facilitate causal reflection and favor systemic learning of business problems. In 1990, the G.I.D.E.A.O. Research Group took action on this idea and centered one of its lines of research on this matter, with three main objectives: a) Creation of TBBSs, b) Introduction of TBBSs in undergraduate and graduate Management courses as well as in executive training, c) Experimentation in controlled environments in order to test the hypothesis mentioned in the above paragraph. Now, ten years after the birth of the idea, we would like to share in this paper the results obtained during that period

Reparto de un dividendo entre empresas del grupo después de sucesivos canjes de valores: Análisis de la consulta 4 del BOICAC 121, de mayo de 2020

Reparto de un dividendo entre empresas del grupo después de sucesivos canjes de valores

Antitumor and antiangiogenic potential of solomonamide synthesis intermediates

Es comunicación (formato panel) a congreso internacionalIn this work we developed a new synthetic strategy towards the solomonamides. a novel class of cyclopeptides of marine origin. The described synthetic approach utilized an olefin metathesis reaction to form the [15]-membered ring contained in these natural products. During the synthetic process, a diverse set of analogues was generated and we evaluated their potential antitumor activity in vitro. For this purpose we performed in vitro proliferation assays, determining the IC50 values of the compounds in a panel of tumor cell lines. In addition, we evaluated the possible antiangiogenic effects of these solomonamide analogues by using in vitro endothelial cell differentiation assays. Our results showed that the potential antitumor and antiangiogenic activity of the studied analogues depended on their chemical structure, suggesting that the presence of specific functional groups could be responsible of their biological activity. Further studies are needed to understand the basis of the observed activities in endothelial and tumor cells.Our experimental work is supported by grants BIO2014-56092-R (MINECO and FEDER) and P12-CTS-1507 (Andalusian Government and FEDER) and funds from group BIO-267 (Andalusian Government). The "CIBER de Enfermedades Raras" is an initiative from the ISCIII (Spain). This communicaction has the support of a travel grant "Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Normas de registro y valoración del inmovilizado material y de las inversiones inmobiliarias. Resolución del ICAC de 1 de marzo de 2013 (I)

On March 8, 2013, it was published in the Boletín Oficial del Estado the Order of the Institute of Accounting and Auditing (ICAC) of March 1, 2013, which establishes rules for registration and valuation of the assets and the property investments. The order is divided into five rules: • First: Initial valuation.• Second: Subsequent valuation.• Third: Special ways of acquisition of fixed assets.• Fourth: Derecognition. • Fifth: Property investments. This paper attempts an analysis of standards and evaluation criteria adopted by the ICAC in that Resolution, through practical application examples that can help the professional and scholar of accounting to a better understanding of these rules.El pasado 8 de marzo de 2013 se publicó en el Boletín Oficial del Estado la Resolución de 1 de marzo de 2013, del Instituto de Contabilidad y Auditoría de Cuentas (ICAC), por la que se dictan normas de registro y valoración del inmovilizado material y de las inversiones inmobiliarias.La resolución se divide en cinco normas: • Primera: Valoración inicial.• Segunda: Valoración posterior.• Tercera: Formas especiales de adquisición del inmovilizado material.• Cuarta: Baja en cuentas.• Quinta: Inversiones inmobiliarias. El presente trabajo pretende realizar un análisis de las normas y criterios de valoración adoptados por el ICAC en la citada resolución, a través de ejemplos de aplicación práctica que puedan ayudar al profesional y estudioso de la contabilidad a una mejor comprensión de estas normas

Consultas planteadas al ICAC

BOICAC núm. 81, marzo 2010. Consulta 3. Moneda extranjera, NRV 11.ª. Operaciones por cuenta ajena. BOICAC núm. 81, marzo 2010. Consulta 4. Reducción de capita

Consultas planteadas al ICAC

BOICAC núm. 77, marzo 2009. Consulta 4. Instrumentos financieros. NRV 9.ª Deterioro de valor de acciones. BOICAC núm. 80, diciembre 2009. Consulta 1. Marco conceptual. Concesiones administrativas. Activos objeto de reposición