Foreword (letter)

Submitted by Sandra Infurna ([email protected]) on 2019-11-28T13:49:22Z No. of bitstreams: 1 AlvaroLuizBertho_IOC_2001.pdf: 4528 bytes, checksum: 728ca4d81e2ed85f56291aaecd8f7059 (MD5)Approved for entry into archive by Sandra Infurna ([email protected]) on 2019-11-28T13:56:56Z (GMT) No. of bitstreams: 1 AlvaroLuizBertho_IOC_2001.pdf: 4528 bytes, checksum: 728ca4d81e2ed85f56291aaecd8f7059 (MD5)Made available in DSpace on 2019-11-28T13:56:56Z (GMT). No. of bitstreams: 1 AlvaroLuizBertho_IOC_2001.pdf: 4528 bytes, checksum: 728ca4d81e2ed85f56291aaecd8f7059 (MD5) Previous issue date: 2000Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.In 1997, Dr Sérgio Coutinho invited me to consider organizing, in 1998, a symposium to commemorate the ten years of flow cytomery in Brazil. The purpose of the meeting was to bring together several professionals who have contributed in someway to the development of this technology in our scientific milieu. The symposium took place between September 29-30, 1998 at Fundação Oswaldo Cruz, Fiocruz, Rio de Janeiro and consisted of round-tables emphasizing the applications of flow citomery to some important fields including cytokines, membrane receptors, cellular functions, cancer and Aids. The scientific content of the meeting made it both unique and very informative. The discussion generated by the presentations highlighted the importance of flow cytomery as a tool for studying several phenomena in clinical as well as basic biological research. Sincere acknowledgments are indispensable to the co-organizers Wilson Savino, Geraldo Pereira, Rodrigo CorreaOliveria and especially to Marta Santiago for her invaluable assistance and extraordinary organizational acumen. We are equally grateful to all the speakers, administratrive assistans and participans Furthermore, none of this would have been possible without the generous financial and moral support provided by the Oswaldo Cruz Institute and Beckman-Colter, Brazil

Foreword

In 1997, Dr Srgio Coutinho invited me to consider organizing, in 1998, a symposium to commemorate the ten years of flow cytomery in Brazil. The purpose of the meeting was to bring together several professionals who have contributed in someway to the development of this technology in our scientific milieu. The symposium took place between September 29-30, 1998 at Fundao Oswaldo Cruz, Fiocruz, Rio de Janeiro and consisted of round-tables emphasizing the applications of flow citomery to some important fields including cytokines, membrane receptors, cellular functions, cancer and Aids. The scientific content of the meeting made it both unique and very informative. The discussion generated by the presentations highlighted the importance of flow cytomery as a tool for studying several phenomena in clinical as well as basic biological research

Foreword

In 1997, Dr Srgio Coutinho invited me to consider organizing, in 1998, a symposium to commemorate the ten years of flow cytomery in Brazil. The purpose of the meeting was to bring together several professionals who have contributed in someway to the development of this technology in our scientific milieu. The symposium took place between September 29-30, 1998 at Fundao Oswaldo Cruz, Fiocruz, Rio de Janeiro and consisted of round-tables emphasizing the applications of flow citomery to some important fields including cytokines, membrane receptors, cellular functions, cancer and Aids. The scientific content of the meeting made it both unique and very informative. The discussion generated by the presentations highlighted the importance of flow cytomery as a tool for studying several phenomena in clinical as well as basic biological research

The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets

The objective of this paper is to propose a protocol to analyze blood samples in yellow fever 17DD vaccinated which developed serious adverse events. We investigated whether or not the time between sample collection and sample processing could interfere in lymphocyte subset percentage, for it is often impossible to analyze blood samples immediately after collection due to transport delay from collection places to the flow cytometry facility. CD4+CD38+ T, CD8+CD38+ T, CD3+ T, CD19+ B lymphocyte subsets were analyzed by flow cytometry in nine healthy volunteers immediately after blood collection and after intervals of 24 and 48 h. The whole blood lysis method and gradient sedimentation by Histopaque were applied to isolate peripheral blood mononuclear cells for flow cytometry analyses. With the lysis method, there was no significant change in lymphocyte subset percentage between the two time intervals (24 and 48 h). In contrast, when blood samples were processed by Histopaque gradient sedimentation, time intervals for sample processing influenced the percentage in T lymphocyte subsets but not in B cells. From the results obtained, we could conclude that the whole blood lysis method is more appropriate than gradient sedimentation by Histopaque for immunophenotyping of blood samples collected after serious adverse events, due to less variation in the lymphocyte subset levels with respect to the time factor

Lymphocyte subset analyses in healthy adults vaccinated with yellow fever 17DD virus

In this study the kinetics of humoral and cellular immune responses in first-time vaccinees and re-vaccinees with the yellow fever 17DD vaccine virus was analyzed. Flow cytometric analyses were used to determine percentual values of T and B cells in parallel to the yellow fever neutralizing antibody production. All lymphocyte subsets analyzed were augmented around the 30th post vaccination day, both for first-time vaccinees and re-vaccinees. CD3+ T cells increased from 30.8% (SE ± 4%) to 61.15% (SE ± 4.2%), CD4+ T cells from 22.4% (SE ± 3.6%) to 39.17% (SE ± 2%) with 43% of these cells corresponding to CD4+CD45RO+ T cells, CD8+ T cells from 15.2% (SE ± 2.9%) to 27% (SE ± 3%) with 70% corresponding to CD8+CD45RO+ T cells in first-time vaccinees. In re-vaccinees, the CD3+ T cells increased from 50.7% (SE ± 3%) to 80% (SE ± 2.3%), CD4+ T cells from 24.9% (SE ± 1.4%) to 40% (SE ± 3%) presenting a percentage of 95% CD4+CD45RO+ T cells, CD8+ T cells from 19.7% (SE ± 1.8%) to 25% (SE ± 2%). Among CD8+CD38+ T cells there could be observed an increase from 15 to 41.6% in first-time vaccinees and 20.7 to 62.6% in re-vaccinees. Regarding neutralizing antibodies, the re-vaccinees presented high titers even before re-vaccination. The levels of neutralizing antibodies of first-time vaccinees were similar to those presented by re-vaccinees at day 30 after vaccination, indicating the success of primary vaccination. Our data provide a basis for further studies on immunological behavior of the YF 17DD vaccine

Detection of Intracytoplasmic Cytokines by Flow Cytometry

Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models

Immunophenotyping in Saliva as an Alternative Approach for Evaluation of Immunopathogenesis in Chronic Periodontitis

Made available in DSpace on 2015-05-27T13:39:47Z (GMT). No. of bitstreams: 2 license.txt: 1914 bytes, checksum: 7d48279ffeed55da8dfe2f8e81f3b81f (MD5) raquel_ferrazetal_IOC_2014.pdf: 1083647 bytes, checksum: 0cfdc88dfdfe0baee8dc35638525e5b8 (MD5) Previous issue date: 2014Universidade do Estado do Amazonas (UEA). Escola de Ciências da Saúde. Faculdade de Odontologia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação Celular. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Leônidas e Maria Deane. Departamento de Biodiversidade em Saúde. Manaus, Am, Brasil.Universidade Federal do Amazonas (UFA). Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imunologia. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo – Purificação Celular. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Amazonas (UFA). Instituto de Ciências Biológicas. Departamento de Parasitologia. Laboratório de Imunologia. Manaus, AM, Brasil.Background: To date, flow cytometric immunophenotyping has not been used to investigate immune patterns in saliva samples from individuals with inflammatory processes in the oral cavity, such as chronic periodontitis (CP). Saliva analysis could be a non-invasive method for evaluating oral health. The objective of this study is to determine the phenotype of leukocytes and total immunoglobulin A (IgA), IgG, and IgM titers in the saliva of individuals with CP. Methods: Saliva samples were obtained from patients with CP (n = 12) and from a control group (n = 27) without oral diseases. Flow cytometry was performed to determine the frequency of T cells (CD4+ and CD8+), B cells, and natural killer (NK) cells as well as the total leukocyte population. Immunoglobulin titers were determined by dot enzyme-linked immunosorbent assay. Results: Cell immunophenotyping revealed that patients with CP had a higher frequency of total leukocytes (47.94% ± 5.1%; P < 0.001), B cells (43.93% ± 6.2%; P = 0.006), NK cells (0.16% ± 0.04%; P = 0.03), and CD4+ T cells (38.99% ± 4.4%; P = 0.002) than individuals without oral pathologies (24.75% ± 2.2%, 20.60% ± 2.7%, 0.09% ± 0.03%, and 16.82% ± 3.5%, respectively). No significant differences in salivary total IgA, IgG, and IgM titers were found between the two cohorts studied. Nevertheless, higher total IgG levels were observed in patients with CP, which could indicate a possible correlation between clinical attachment level and salivary IgG (P = 0.07; r2 = 0.08). Conclusion: These results show that cell phenotyping by flow cytometry could be an effective tool for determining leukocyte profiles in saliva samples from patients with CP and healthy individuals