3 research outputs found
Epidemiology of hepatitis B virus infection among recyclable waste collectors in central Brazil
Genotyping of hepatitis C virus and evaluation of treatment response in Goiânia-Goiás, with emphasis on the polymorphism upstream of IL28B gene
Submitted by Marlene Santos ([email protected]) on 2014-11-27T19:29:54Z
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Dissertação - Ágabo Macêdo da Costa e Silva- 2014.pdf: 1890977 bytes, checksum: 8a4d7bcee9ab084556c93c3c72e6a9f8 (MD5)
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Previous issue date: 2014-02-28Fundação de Amparo à Pesquisa do Estado de Goiás - FAPEGHepatitis C represents a public health problem worldwide. Hepatitis C virus (HCV) is
classified into seven genotypes and many subtypes. Besides their epidemiological
importance, these genotypes have influence on the response to hepatitis C treatment, as
well as other factors related to the virus and its host, such as polymorphisms upstream
of interleukin (IL) 28B locus. Despite the importance of these factors in the response to
treatment of hepatitis C, there are no data regarding the subject in the Midwest Region
of Brazil. The present study aimed to identify the genotypes of HCV among patients
attended at a reference laboratory in Goiânia-GO, and also to assess response to
treatment of patients infected with genotype 1 with pegylated interferon (PEG-IFN) and
ribavirin (RBV), with emphasis on the polymorphism upstream of IL28B gene (rs
12979860). For HCV genotyping, a cross-sectional study was conduct in an anti-HCV
positive patients in a reference laboratory in Goiânia-GO, during in a period of 10 years
(2003 to 2012) (n = 1300). From January/2012 to December/2013 (n = 101), a cohort
was conduct among patients infected with HCV genotype 1 treated with PEG-IFN and
RBV in order to evaluate treatment response. Patients were interviewed and blood
samples collected for detection of viral RNA by polymerase chain reaction (PCR) with
primers complementary to the region 5’ noncoding (NC) of HCV. All HCV RNA
positive samples were genotyped by line probe assay – LiPA, and the samples typed as
1a/1b, 2 and 4 were sequenced in the NS5B region of the viral genome. The analysis of
single nucleotide polymorphism (SNP) rs12979860 C/T was performed by restriction
fragment length polymorphism (RFLP). Of the 1300 samples tested for HCV RNA, 894
were positive. Among these, genotypes 1 (76.3%), 2 (1.9%), 3 (21.6%) and 4 (0.2%)
were found. These data show the predominance of genotype 1, followed by 3 and 2, and
support the results of other studies in Goiânia. In addition, genotype 4 was found in the
studied population. Regarding to SNP rs12979860 C/T, the TC genotype was the most
common (57.4%), followed by CC (23.8%) and TT (18.8%). The rate of sustained
virologic response (SVR) was 28.7%, being higher in individuals with the CC genotype
(54.2%) when compared to those with CT and TT genotypes (22.4% and 15.8%,
respectively). These results indicate that analysis of SNP rs12979860 is an important
predictor of SVR following PEG-IFN and RBV treatment in patients infected with HCV
genotype 1 in our region.A hepatite C representa um problema de saúde pública mundial. O vírus da hepatite C
(HCV) é classificado em sete genótipos e vários subtipos. Além da importância
epidemiológica, os genótipos desse vírus influenciam a resposta ao tratamento da
hepatite C, assim como outros fatores relacionados ao vírus e ao hospedeiro, como o
polimorfismo relacionado ao gene da interleucina (IL) 28B. Apesar da importância
desses fatores na resposta ao tratamento da hepatite C, não existem dados sobre o tema
na Região Centro-Oeste. O presente estudo teve como objetivos identificar os genótipos
do HCV em pacientes atendidos em laboratório de referência em Goiânia-GO e avaliar
a resposta ao tratamento com interferon peguilado (PEG IFN) e ribavirina (RBV) em
portadores do genótipo 1, com ênfase no polimorfismo relacionado ao gene IL28B (rs
12979860). Foi realizado um estudo de corte transversal para genotipagem do HCV em
pacientes anti-HCV reagentes atendidos em laboratório de referência em Goiânia-GO,
em um período de 10 anos (2003 a 2012) (n = 1300). Entre janeiro/2012 e
dezembro/2013 (n = 101), foi formada uma coorte de portadores do genótipo 1 do HCV
submetidos ao tratamento da hepatite C com PEG IFN e RBV, para avaliação de sua
resposta. Os pacientes foram entrevistados e amostras sanguíneas coletadas para
detecção do RNA viral por reação em cadeia da polimerase (PCR) com iniciadores
complementares a região 5’ não codificante (NC) do HCV. Todas as amostras RNAHCV
positivas foram genotipadas por line probe assay – LiPA, e as tipadas como
1a/1b, 2 e 4 foram sequenciadas para a região NS5B do genoma viral. A análise do
single nucleotide polymorphism (SNP) rs12979860 C/T foi realizada por restriction
fragment length polymorphism (RFLP). Das 1300 amostras testadas para RNA-HCV,
894 foram positivas, sendo identificados os genótipos 1 (76,3%), 2 (1,9%), 3 (21,6%) e
4 (0,2%). Estes dados mostram o predomínio do genótipo 1, seguido do 3 e 2, e
corroboram os de outros estudos realizados em Goiânia, com a adição do genótipo 4 na
população estudada. Em relação ao SNP rs12979860 C/T, o genótipo TC foi o mais
frequente (57,4%), seguido por CC (23,8%) e TT (18,8%). A taxa de resposta virológica
sustentada (RVS) foi de 28,7%, sendo superior nos portadores do genótipo CC (54,2%)
em relação aos genótipos TC e TT (22,4% e 15,8%, respectivamente). Estes resultados
indicam que a análise do SNP rs12979860 é importante preditor de RVS ao tratamento
com PEG IFN e RBV em pacientes infectados com o genótipo 1 do HCV em nossa
região
Epidemiology of hepatitis B virus infection among recyclable waste collectors in central Brazil
Submitted by Luciana Ferreira ([email protected]) on 2018-07-31T13:28:51Z
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Artigo - Tamíris Augusto Marinho - 2014.pdf: 463246 bytes, checksum: 33b373a77e4444b2ae399c74d3bb1ce7 (MD5)
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Artigo - Tamíris Augusto Marinho - 2014.pdf: 463246 bytes, checksum: 33b373a77e4444b2ae399c74d3bb1ce7 (MD5)
license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2018-08-01T14:13:26Z (GMT). No. of bitstreams: 2
Artigo - Tamíris Augusto Marinho - 2014.pdf: 463246 bytes, checksum: 33b373a77e4444b2ae399c74d3bb1ce7 (MD5)
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Previous issue date: 2014-02The collection of recyclable waste materials is a widespread activity among the urban poor. Today, this occupation
attracts an increasingly large number of individuals. Despite its economic and environmental importance, this activity is associated
with unsafe and unhealthy working conditions. The aim of this study was to investigate the seroepidemiological profi le of hepatitis
B virus (HBV) infection in a population of recyclable waste collectors in central Brazil. Methods: Recyclable waste collectors from
all 15 recycling cooperatives in Goiânia City were invited to participate in the study. The participants (n = 431) were interviewed
and screened for hepatitis B surface antigen (HBsAg) and antibodies against HBsAg (anti-HBs) and hepatitis B core antigen
(anti-HBc) by enzyme-linked immunosorbent assay (ELISA). HBsAg- and anti-HBc-positive samples were tested for HBV DNA
and genotyped. Results: The overall prevalence of HBV infection (HBsAg- and/or anti-HBc-positive) was 12.8%. An age over
40 years and illicit drug use were associated with HBV infection. HBV DNA was detected in 2/3 HBsAg-positive samples and in
1/52 anti-HBc-positive/HBsAg-negative samples (an occult HBV infection rate of 1.9%), in which the genotypes/subgenotypes
A/A1, D/D3 and F/F2 were identifi ed. Only 12.3% of the recyclable waste collectors had serological evidence of previous HBV
vaccination. Conclusions: These fi ndings highlight the vulnerability of recyclable waste collectors to HBV infection and reinforce
the importance of public health policies that address the health and safety of this socially vulnerable population