MICs of the lipopeptides derived from BP100 against the bacteria <i>E</i>. <i>amylovora</i> (<i>Ea</i>), <i>P</i>. <i>syringae</i> pv. syringae (<i>Pss</i>), <i>P</i>. <i>syringae</i> pv. actinidiae (<i>Psa</i>), <i>X</i>. <i>arboricola</i> pv. pruni (<i>Xap</i>), <i>X</i>. <i>fragariae</i> (<i>Xf</i>), and <i>X</i>. <i>axonopodis</i> pv. vesicatoria (<i>Xav</i>), and the fungi <i>P</i>. <i>expansum</i> (<i>Pe</i>) and <i>F</i>. <i>oxysporum</i> (<i>Fo</i>).

<p>The position of the acyl group at the N-terminus is indicated by a N, and the position of the acylated Lys by numbers 1 to 11. The type of the acyl group is depicted as R = COC₅H₁₁ (hexanoyl), R = COC₃H₇ (butanoyl) and R = COC₁₁H₂₃ (lauroyl).</p

Sequences, retention times and purities on HPLC, and mass spectrometry data of lipopeptides.

<p>Sequences, retention times and purities on HPLC, and mass spectrometry data of lipopeptides.</p

Lipopeptides with the best biological activity profile.

<p>Lipopeptides with the best biological activity profile.</p

Hemolytic activity of the lipopeptides derived from BP100 at 250 μM.

<p>Vertical bars within each column indicate confidence interval at the mean. The position of the acyl group at the N-terminus is indicated by a N, and the position of the acylated Lys by numbers 1 to 11. The type of the acyl group is depicted as R = COC₅H₁₁ (hexanoyl), R = COC₃H₇ (butanoyl) and R = COC₁₁H₂₃ (lauroyl).</p

Effect of the lipopeptides derived from BP100 at 50 and 250 μM on the size of the lesions in infiltrated tobacco leaves.

<p>This effect was compared to melittin. Vertical bars within each column indicate confidence interval at the mean. The position of the acyl group at the N-terminus is indicated by a N, and the position of the acylated Lys by numbers 1 to 11. The type of the acyl group is depicted as R = COC₅H₁₁ (hexanoyl), R = COC₃H₇ (butanoyl) and R = COC₁₁H₂₃ (lauroyl).</p

Susceptibility of BP100 and selected lipopeptides to proteinase K hydrolysis.

<p>Lipopeptides were treated with proteinase K for 60 min and the percentage of digestion was analyzed by HPLC.</p

Expression of genes related to defense/stress response in tomato after the treatment with the reference products flagellin 15 (flg15), jasmonic acid (JA), and acybenzolar-<i>S</i>-methyl (ASM), and with BP100 and 9 selected lipopeptides.

<p>Fold induction above 2 is considered overexpression in the relative quantification by the <i>ΔΔCt</i> method. Significant values are indicated in bold.</p