PENGARUH PEMBERIAN SUPLEMEN BUBUK TULANG AYAM SELAMA MASA KEHAMILAN DAN MENYUSUI TERHADAP KADAR KALSIUM GIGI ANAK (Kajian in vivo pada tikus putih galur Sprague dawley)

Calcium is one of important mineral in the growth of tooth and bone. The intake of additional calcium from diet may help to maintain the level of calcium during pregnancy and lactation periods. Chicken bone contains high calcium and can be used as a source of natural calcium due to its bioavailability. This study was conducted to investigate the effect of chicken bone powder during pregnancy and lactation period of mother rat on calcium level of Sprague dawley pups� teeth. Six Sprague dawley female rats aged 3 months (200-250 g body weight) were divided into 2 groups: treatment and control groups. Treatment group were given chicken bone powder supplement 1375 mg/kg rat�s body weight in 2.5% CMC-Na from the first day of pregnancy up to fifteenth day after the delivery of the pups. Control group were given CMC-Na 2.5%. Two ml of suspension was given by intragastric administration once a day. On the fifteenth day, the rat pups were sacrificed and the teeth of both groups were collected. The calcium level of the tooth was assayed by atomic absorption spectrophotometry (AAS) method on the wave length of 422.7 nm. The data were analyzed using independent sample t-test to determine the significance of mean between two different groups. The results showed that the teeth calcium level of treatment group was significantly higher than the control group (p=0.003). It is concluded that the administration of chicken bone powder during pregnancy and lactation period of mother rat increases the calcium level of Sprague dawley pups� teeth