Rapid enamel deposition on Sprague Dawley after nano calcium supplementation during pregnancy

Calcium is one of the most important minerals needed during hard tissue development. The preparation of this material into nano-sized particle is carried out to enhance the bioavailability and distribution of calcium in the body. Lack of calcium during odontogenesis causes defect in enamel such as hypoplasia and hypomineralization. During amelogenesis, after secretion of organic matrices, enamel mineralization will start in the presence of calcium. The objective of this study was to determine the effect of nano calcium supplementation during pregnancy on enamel development. In this study, 3-month-old female Sprague Dawley were mated and divided into three groups: nano calcium group (A), micro calcium group (B), and negative control group (C). The treatment was started on day 1 of pregnancy to day 1 after birth by intragastric administration method. The mandibles of 6 pups from each group were collected and stained with hematoxylin and eosin. Examination was conducted using microscope. Enamel deposition was measured using Optilab Image Raster® and the data collected was analyzed using t-test. Histological section of mandibular right first molar on Sprague Dawley newborn pups showed that enamel was observed on day 1 after birth but only on the group treated with nano calcium and micro calcium. Statistical analysis performed showed that the difference between the two groups was significant (p<0.05). From this study it can be concluded that the administration of nano calcium during pregnancy leads to rapid enamel deposition on Sprague Dawley pups

PENGARUH PEMBERIAN BUBUK TULANG AYAM PADA INDUK TIKUS Sprague dawley SELAMA MASA KEHAMILAN DAN MENYUSUI TERHADAP KETEBALAN DENTIN GIGI ANAKAN

Nutritional deficiency during pregnancy may impair children�s tooth formation. Chicken bones contains various proteins and minerals that can be used as an additional nutrient provider. The purpose of this study was to determine the effect of chicken bone powder administered on Sprague dawley rats during pregnancy and lactation on pups� dentin thickness. Nine Sprague dawley female rats aged 10-12 weeks with approximately weight 200-250 g were divided into 2 groups: a control group consisted of a sub-group of negative control (A) and comparator (B), and a treatment group (C). Since the first day of pregnancy until the pups were 5 days old, each sub group and group were intragastric administered with 2 ml of suspension per day. Suspension of 2,5% CMC-Na, 1,5% of multivitamins and minerals, and 13,75% of chicken bone powder was given to group A, B, and C, consecutively. At the age of 5 days old, four rat pups of each group were sacrificed, the mandibles were collected, histologicaly processed, and stained with HE. The observation of the first molar tooth dentin thickness was done under light microscope in the magnification of 100 x. The data was analyzed using ANOVA and LSD post-hoc test (p<0,05). The administration of chicken bone powder during pregnancy and lactation on the mother rats showed a significant effect to the pup�s dentin thickness. It is concluded that the administration of chicken powder in mother rats during pregnancy and lactation may increase the thickness of dentin on rat pups