VALIDASI PROSEDUR ANALISIS DELTAMETHRIN PADA HATI AYAM MENGGUNAKAN ALAT KROMATOGRAFI CAIR KINERJA TINGGI (KCKT)

Deltamethrin as a pesticide widely used in agriculture and estate sector. Residue of pesticide in food derivate from animals (included offal) has been reported in Indonesia and it would be harmful for health if it is exceeded a predetermined threshold. It caused analysis pesticide need to do. High Performance Liquid Chromatography (HPLC) is one of developing technology analysis pesticide�s residue. High performance liquid chromatography instrument adequate of analyze a variety of shots both qualitatively and quantitatively, either single or mixture component of pesticide. An object of this study is validate the methods of analysis deltamethrin using HPLC instrument based on the specificity, precision, accuracy, limit of detection, limit of quantification, and linearity. The main material used is set HPLC, Shimadzu 6.1, deltamethrin, and chicken�s liver. The support materials is acetonitrile, distilled water, test tubes, spuid, parafilm, vortex mixer, ultrasonic bath, shaker, timers, scales, micropipette, and HPLC injector. Methods of study with smooth liver tissue was then added deltamethrin at a concentration of 1 mg 1 μg/ml, 2 μg/ml, dan 5 μg/ml in a test tube. Cap test tube close with parafilm, and then each in a vortex for 1 minute, put in an ultrasonic bath for 15 minute at 40 0C, then homogenization in shaker with a speed of 150-200 beat/minute during 20 minutes. Then all the tubes centrifuged during 12 minutes at speed 13,000 rpm. The supernatant was taken and injection to HPLC. Results showed the mean cup of deltamethrin separated from other compounds in the retention time between 8 to 9.5 minutes at a concentration of 1 μg/ml, 2 μg/ml, and 5 μg/ml in the test specificity. Indicated precision for each area concentration in a series are 43.01