Functional Recovery and Serum Angiogenin Changes According to Intensity of Rehabilitation Therapy After Stroke

Rehabilitation is still the only treatment available to improve functional status after the acute phase of stroke. Most clinical guidelines highlight the need to design rehabilitation treatments considering starting time, intensity, and frequency, according to the tolerance of the patient. However, there are no homogeneous protocols and the biological effects are under investigation. To investigate the impact of rehabilitation intensity (hours) after stroke on functional improvement and serum angiogenin (ANG) in a 6-month follow-up study. A prospective, observational, longitudinal, and multicenter study with three cohorts: strokes in intensive rehabilitation therapy (IRT, minimum 15 h/week) vs. conventional therapy (NO-IRT, <15 h/week), and controls subjects (without known neurological, malignant, or inflammatory diseases). A total of seven centers participated, with functional evaluations and blood sampling during follow-up. The final cohort includes 62 strokes and 43 controls with demographic, clinical, blood samples, and exhaustive functional monitoring. The median (IQR) number of weekly hours of therapy was different: IRT 15 (15-16) vs. NO-IRT 7.5 (5-9), p < 0.01, with progressive and significant improvements in both groups. However, IRT patients showed earlier improvements (within 1 month) on several scales (CAHAI, FMA, and FAC; p < 0.001) and the earliest community ambulation achievements (0.89 m/s at 3 months). There was a significant difference in ANG temporal profile between the IRT and NO-IRT groups (p < 0.01). Additionally, ANG was elevated at 1 month only in the IRT group (p < 0.05) whereas it decreased in the NO-IRT group (p < 0.05). Our results suggest an association of rehabilitation intensity with early functional improvements, and connect the rehabilitation process with blood biomarkers

Attomolar Detection of Botulinum Toxin Type A in Complex Biological Matrices

BACKGROUND: A highly sensitive, rapid and cost efficient method that can detect active botulinum neurotoxin (BoNT) in complex biological samples such as foods or serum is desired in order to 1) counter the potential bioterrorist threat 2) enhance food safety 3) enable future pharmacokinetic studies in medical applications that utilize BoNTs. METHODOLOGY/PRINCIPAL FINDINGS: Here we describe a botulinum neurotoxin serotype A assay with a large immuno-sorbent surface area (BoNT/A ALISSA) that captures a low number of toxin molecules and measures their intrinsic metalloprotease activity with a fluorogenic substrate. In direct comparison with the "gold standard" mouse bioassay, the ALISSA is four to five orders of magnitudes more sensitive and considerably faster. Our method reaches attomolar sensitivities in serum, milk, carrot juice, and in the diluent fluid used in the mouse assay. ALISSA has high specificity for the targeted type A toxin when tested against alternative proteases including other BoNT serotypes and trypsin, and it detects the holotoxin as well as the multi-protein complex form of BoNT/A. The assay was optimized for temperature, substrate concentration, size and volume proportions of the immuno-sorbent matrix, enrichment and reaction times. Finally, a kinetic model is presented that is consistent with the observed improvement in sensitivity. CONCLUSIONS/SIGNIFICANCE: The sensitivity, specificity, speed and simplicity of the BoNT ALISSA should make this method attractive for diagnostic, biodefense and pharmacological applications

DRD4 Polymorphism Moderates the Effect of Alcohol Consumption on Social Bonding

Development of interpersonal relationships is a fundamental human motivation, and behaviors facilitating social bonding are prized. Some individuals experience enhanced reward from alcohol in social contexts and may be at heightened risk for developing and maintaining problematic drinking. We employed a 3 (group beverage condition) ×2 (genotype) design (N = 422) to test the moderating influence of the dopamine D4 receptor gene (DRD4 VNTR) polymorphism on the effects of alcohol on social bonding. A significant gene x environment interaction showed that carriers of at least one copy of the 7-repeat allele reported higher social bonding in the alcohol, relative to placebo or control conditions, whereas alcohol did not affect ratings of 7-absent allele carriers. Carriers of the 7-repeat allele were especially sensitive to alcohol's effects on social bonding. These data converge with other recent gene-environment interaction findings implicating the DRD4 polymorphism in the development of alcohol use disorders, and results suggest a specific pathway by which social factors may increase risk for problematic drinking among 7-repeat carriers. More generally, our findings highlight the potential utility of employing transdisciplinary methods that integrate genetic methodologies, social psychology, and addiction theory to improve theories of alcohol use and abuse

10 tips to using video analysis more effectively in physical education

In order for video replay to be an effective assessment and teaching tool, a number of steps need to be followed. This article provides some useful tips for physical educators to ensure they are implementing this video technology effectively when teaching the overarm throw

Igniting the pressure acclimatization training debate: contradictory evidence from Australian football

In Australian Football, set shot goal kicking is when a player has a shot at goal after taking a mark (i.e., catching a kicked ball) or receiving a free kick (i.e., penalty from an opposing player). In the past two decades, Australian football has improved in nearly all aspects of the game, yet set shot goal kicking accuracy has declined. The purpose of the current pilot study was to investigate whether pressure acclimatization training improves Australian football goal kicking under pressure. Twelve football players assigned to either an experimental (EG) or a control group (CG) completed a pre-intervention test, intervention, and post-intervention test. During the pre-intervention and post-intervention tests, participants attempted 10 goal kicking trials under low pressure (LP) and high pressure (HP) conditions. After five attempts, participants completed an anxiety questionnaire. During the intervention period, the EG practiced under HP, while the CG practiced under LP. These pilot results indicated a significant increase in participants ' anxiety from LP to HP for both groups and a significant decrease in accuracy from pre-intervention to post-intervention. In the post-intervention test, the CG was more accurate under HP than the EG, indicating no significant advantage by practicing under pressure and contradicting other acclimatization studies. These results may ignite a debate about the benefits of acclimatization training, but caution should be exercised when interpreting the results considering the pilot nature of the study. Larger sample sizes should be used to further explore these effects. Future research in acclimatization training is discussed. (PsycINFO Database Record (c) 2016 APA, all rights reserved

Validation of a Follow-Through Developmental Sequence for the Overarm Throw for Force in University Students

Background: The purpose of this study was to examine the effect of the follow-through on thrown ball velocity, potentially justifying inclusion of the follow-through in Roberton’s five critical components. Method: Seventy-eight University students participated in the overarm, dominant hand, throwing task, which involved throwing a standard tennis ball with maximum force three times. Each throw was filmed by two cameras placed behind and to the open side of the thrower to assess the throwing technique. The velocity of the throws was recorded with a radar gun. Results: Results indicated that, after accounting for the effects of gender, age, and throwing experience, there was a significant effect of follow-through level on throw velocity. Analysis of covariance also revealed a significant gender effect, with males throwing significantly faster than females. Results indicated the follow-through had the second largest impact on thrown ball velocity of all six components. Discussion: These findings provide preliminary support that the follow-through should be added to Roberton’s developmental levels. The inclusion of the follow-through component could assist teachers and coaches to facilitate learner and athlete development and could also improve the accuracy of throwing development assessment