The heterotrimeric G-protein complex modulates light sensitivity in arabidopsis thaliana seed germination

Release of dormancy and induction of seed germination are complex traits finely regulated by hormonal signals and environmental cues such as temperature and light. The Red (R):Far-Red (FR) phytochrome photoreceptors mediate light regulation of seed germination. We investigated the possible involvement of heterotrimeric G-protein complex in the phytochrome signaling pathways of Arabidopsis thaliana seed germination. Germination rates of null mutants of the alpha (Gα) and beta (Gβ) subunits of the G-protein (Atgpa1-4 and agb1-2, respectively) and the double mutant (agb1-2/gpa1-4) are lower than the wildtype (WT) under continuous or pulsed light. The Gα and Gβ subunits play a role in seed germination under hourly pulses of R lower than 0.1 μmol m -2 s -1 whereas the Gβ subunit plays a role in higher R fluences. The germination of double mutants of G-protein subunits with phyA-211 and phyB-9 suggests that AtGPA1 seems to act as a positive regulator of phyA and probably phyB signaling pathways, while the role of AGB1 is ambiguous. The imbibition of seeds at 4°C and 35°C alters the R and FR light responsiveness of WT and G-protein mutants to a similar magnitude. Thus, Gα and Gβ subunits of the heterotrimeric G-protein complex modulate light induction of seed germination by phytochromes and are dispensable for the control of dormancy by low and high temperatures prior to irradiation. We discuss the possible indirect role of the G-protein complex on the phytochrome-regulated germination through hormonal signaling pathways.Fil: Botto, Javier Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Ibarra, Silvia Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Jones, Alan M.. University of North Carolina; Estados Unido

Arabidopsis heterotrimeric G-protein regulates cell wall defense and resistance to necrotrophic fungi

The Arabidopsis heterotrimeric G-protein controls defense responses to necrotrophic and vascular fungi. The agb1 mutant impaired in the Gβ subunit displays enhanced susceptibility to these pathogens. Gβ/AGB1 forms an obligate dimer with either one of the Arabidopsis Gγ subunits (γ1/AGG1 and γ2/AGG2). Accordingly, we now demonstrate that the agg1 agg2 double mutant is as susceptible as agb1 plants to the necrotrophic fungus Plectosphaerella cucumerina. To elucidate the molecular basis of heterotrimeric G-protein-mediated resistance, we performed a comparative transcriptomic analysis of agb1-1 mutant and wild-type plants upon inoculation with P. cucumerina. This analysis, together with metabolomic studies, demonstrated that G-protein-mediated resistance was independent of defensive pathways required for resistance to necrotrophic fungi, such as the salicylic acid, jasmonic acid, ethylene, abscisic acid, and tryptophan-derived metabolites signaling, as these pathways were not impaired in agb1 and agg1 agg2 mutants. Notably, many mis-regulated genes in agb1 plants were related with cell wall functions, which was also the case in agg1 agg2 mutant. Biochemical analyses and Fourier Transform InfraRed (FTIR) spectroscopy of cell walls from G-protein mutants revealed that the xylose content was lower in agb1 and agg1 agg2 mutants than in wild-type plants, and that mutant walls had similar FTIR spectratypes, which differed from that of wild-type plants. The data presented here suggest a canonical functionality of the Gβ and Gγ1/γ2 subunits in the control of Arabidopsis immune responses and the regulation of cell wall composition

Optically visible post-AGB/RGB stars and young stellar objects in the Small Magellanic Cloud: candidate selection, spectral energy distributions and spectroscopic examination

We have carried out a search for optically visible post-AGB candidates in the Small Magellanic Cloud (SMC). We used mid-IR observations from the Spitzer Space Telescope to select optically visible candidates with a mid-IR excess. We obtained low-resolution optical spectra for 801 candidates. After removing contaminants and poor quality spectra, the final sample comprised of 63 post-AGB/RGB candidates of A - F spectral type. Using the spectra, we estimated the stellar parameters: effective temperature, surface gravity and [Fe/H]. We also estimated the reddening and deduced the luminosity using the stellar parameters combined with photometry. Based on a luminosity criterion, 42 of these 63 sources were classified as post-RGB candidates and the remaining as post-AGB candidates. From the spectral energy distributions we found that 6 of the 63 post-AGB/RGB candidates have a circumstellar shell suggesting that they are single stars, while 27 of them have a surrounding disc, suggesting that they are binaries. For the remaining candidates the nature of the circumstellar environment was unclear. Variability is displayed by 38 post-AGB/RGB candidates with common variability types being the Population II Cepheids (including RV-Tauri stars) and semi-regular variables. This study has also revealed a new s-process enriched RV Tauri star (J005107.19-734133.3). From the numbers of post-AGB/RGB stars in the SMC, we were able to estimate evolutionary rates that are in good agreement with the stellar evolution models with mass loss in the post-AGB phase and re-accretion in the post-RGB phase. This study also resulted in a new sample of 40 luminous young stellar objects (YSOs) of A - F spectral type. Additionally, we also identified a group of 63 objects whose spectra are dominated by emission lines and in some cases, a UV continuum. These objects are likely to be either hot post-AGB/RGBs or luminous YSOs.Comment: 67 pages, 26 figures, 20 tables, 3 appendices + online supporting information on CD

Lossless Compression Based on a Discrete and Exact Radon Transform: A Preliminary Study

International audienceThis article presents a new lossless compression algorithm based on a Finite Radon Transform. The baseline of this work consist in encoding the difference between several sim- ilar finite Radon projections. Two differential encoding tech- niques are needed to efficiently encode the Radon trans- formed data. An intra-projection encoding technique is first performed, this differential encoding process takes benefit of the redundancy present in such projections. The second step consists in encoding the similarities between Radon projections, this is the inter-projection encoding procedure. The used Finite Radon transform (called Mojette transform), allows to ensure both compression and data redundancy. The main novelty of this work is the use of a secure dis- tributed storage or transmission tool in a lossless compres- sion context

A Regulatory Module Controlling Homeostasis of a Plant Immune Kinase

Plant pattern recognition receptors (PRRs) perceive microbial and endogenous molecular patterns to activate immune signaling. The cytoplasmic kinase BIK1 acts downstream of multiple PRRs as a rate-limiting component, whose phosphorylation and accumulation are central to immune signal propagation. Previous work identified the calcium-dependent protein kinase CPK28 and heterotrimeric G proteins as negative and positive regulators of BIK1 accumulation, respectively. However, mechanisms underlying this regulation remain unknown. Here we show that the plant U-box proteins PUB25 and PUB26 are homologous E3 ligases that mark BIK1 for degradation to negatively regulate immunity. We demonstrate that the heterotrimeric G proteins inhibit PUB25/26 activity to stabilize BIK1, whereas CPK28 specifically phosphorylates conserved residues in PUB25/26 to enhance their activity and promote BIK1 degradation. Interestingly, PUB25/26 specifically target non-activated BIK1, suggesting that activated BIK1 is maintained for immune signaling. Our findings reveal a multi-protein regulatory module that enables robust yet tightly regulated immune responses

The range of validity of cluster masses and ages derived from broad-band photometry

I analyze the stochastic effects introduced by the sampling of the stellar initial mass function (SIMF) in the derivation of the individual masses and the cluster mass function (CMF) from broad-band visible-NIR unresolved photometry. The classical method of using unweighted UBV photometry to simultaneously establish ages and extinctions of stellar clusters is found to be unreliable for clusters older than approx. 30 Ma, even for relatively large cluster masses. On the other hand, augmenting the filter set to include longer-wavelength filters and using weights for each filter increases the range of masses and ages that can be accurately measured with unresolved photometry. Nevertheless, a relatively large range of masses and ages is found to be dominated by SIMF sampling effects that render the observed masses useless, even when using UBVRIJHK photometry.Comment: 6 pages, 5 figures, to appear in IAU Symposium 266 on Star Cluster

A systematic survey for eruptive young stellar objects using mid-infrared photometry

Accretion in young stellar objects (YSOs) is at least partially episodic, i.e. periods with high accretion rates ('bursts') are interspersed by quiescent phases. These bursts manifest themselves as eruptive variability. Here we presenta systematic survey for eruptive YSOs aiming to constrain the frequency of accretion bursts. We compare mid-infrared photometry from Spitzer and WISE separated by ~5 yr for two samples of YSOs, in nearby star-forming regions and in the Galactic plane, each comprising about 4000 young sources. All objects for which the brightness at 3.6 and 4.5 μm is increased by at least 1 mag between the two epochs may be eruptive variables and burst candidates. For these objects, we carry out follow-up observations in the near-infrared. We discover two new eruptive variables in the Galactic plane which could be FU Ori-type objects, with K-band amplitudes of more than 1.5 mag. One object known to undergo an accretion burst, V2492 Cyg, is recovered by our search as well. In addition, the young star ISO-Oph-50, previously suspected to be an eruptive object, is found to be better explained by a disc with varying circumstellar obscuration. In total, the number of burst events in a sample of 4000 YSOs is 1-4. Assuming that all YSOs undergo episodic accretion, this constraint can be used to show that phases of strong accretion (>10-6Mâ?? yr-1) occur in intervals of about 104 yr, most likely between 5000 and 50 000 yr. This is consistent with the dynamical time-scales for outflows, but not with the separations of emission knots in outflows, indicating that episodic accretion could either trigger or stop collimated large-scale outflows. © 2013 The Authors. Published by Oxford University Press on behalf of the Royal Astronomical Society

New Evolutionary Synthesis code. An application to the irregular galaxy NGC 1560

We have developed a new evolutionary synthesis code, which incorporates the output from chemical evolution models. We compare results of this new code with other published codes, and we apply it to the irregular galaxy NGC 1560 using sophisticated chemical evolution models. The code makes important contributions in two areas: a) the building of synthetic populations with time-dependent star formation rates and stellar populations of different metallicities; b) the extension of the set of stellar tracks from the Geneva group by adding the AGB phases for $m_i/M_\odot \geq 0.8$ as well as the very low mass stars. Our code predicts spectra, broad band colors, and Lick indices by using a spectra library, which cover a more complete grid of stellar parameters. The application of the code with the chemical models to the galaxy NGC 1560 constrain the star formation age for its stellar population around 10.0 Gy.Comment: 10 pages, 15 figures, submited to A&

Nonlinear Hydrodynamics from Flow of Retarded Green's Function

We study the radial flow of retarded Green's function of energy-momentum tensor and $R$-current of dual gauge theory in presence of generic higher derivative terms in bulk Lagrangian. These are first order non-linear Riccati equations. We solve these flow equations analytically and obtain second order transport coefficients of boundary plasma. This way of computing transport coefficients has an advantage over usual Kubo approach. The non-linear equation turns out to be a linear first order equation when we study the Green's function perturbatively in momentum. We consider several examples including $Weyl^4$ term and generic four derivative terms in bulk. We also study the flow equations for $R$-charged black holes and obtain exact expressions for second order transport coefficients for dual plasma in presence of arbitrary chemical potentials. Finally we obtain higher derivative corrections to second order transport coefficients of boundary theory dual to five dimensional gauge supergravity.Comment: Version 2, reference added, typos correcte