Electro-optic and Acousto-optic Laser Beam Scanners

Optical solid state deflectors rely on the electro-optical or acousto-optic effect. These Electro-Optical Deflectors (EODs) and Acousto-Optical Deflectors (AODs) do not contain moving parts and therefore exhibit high deflection velocities and are free of drawbacks associated with mechanical scanners. A description of the principles of operation of EODs and AODs is presented. In addition, characteristics, properties and the (dis)advantages of EODs and AODs, when compared to mirror based mechanical deflectors, is discussed. Deflection angles, speed and accuracy are discussed in terms of resolvable spots and related quantities. Also, response time, damage threshold, efficiency and the type and magnitude of beam distortions is addressed. Optical deflectors are characterized by high angular deflection velocities, but small deflection angles. Whereas mechanical mechanical scanners are characterized by relatively small deflection velocities, but large deflection angles. Arranging an optical deflector and a mechanical scanner in series allows to take advantage of the best of both world

Multiplexed broadband beam steering system utilizing high speed MEMS mirrors

We present a beam steering system based on micro-electromechanical systems technology that features high speed steering of multiple laser beams over a broad wavelength range. By utilizing high speed micromirrors with a broadband metallic coating, our system has the flexibility to simultaneously incorporate a wide range of wavelengths and multiple beams. We demonstrate reconfiguration of two independent beams at different wavelengths (780 and 635 nm) across a common 5x5 array with 4 us settling time. Full simulation of the optical system provides insights on the scalability of the system. Such a system can provide a versatile tool for applications where fast laser multiplexing is necessary.Comment: 11 pages, 6 figures, submitte

Steerable optical tweezers for ultracold atom studies

We report on the implementation of an optical tweezer system for controlled transport of ultracold atoms along a narrow, static confinement channel. The tweezer system is based on high-efficiency acousto-optical deflectors and offers two-dimensional control over beam position. This opens up the possibility for tracking the transport channel when shuttling atomic clouds along the guide, forestalling atom spilling. Multiple clouds can be tracked independently by time-shared tweezer beams addressing individual sites in the channel. The deflectors are controlled using a multichannel direct digital synthesizer, which receives instructions on a sub-microsecond time scale from a field-programmable gate array. Using the tweezer system, we demonstrate sequential binary splitting of an ultracold $\rm^{87}Rb$ cloud into $2^5$ clouds.Comment: 4 pages, 5 figures, 1 movie lin

MICROLENS FOCAL LENGTH CONTROLING FOR OPTICAL TWEEZER ARRAYS BY ACOUSTO-OPTIC MODULATION IN GERMANIUM

The microlens arrays created by ultrasonic waves in acousto-optical material Germanium (Ge) is theoretically proposed. The simulated results show the proposed microlens arrays can be used for optical tweezers arrays to trap an assembly micro-beads. Moreover, with the control by changing of the ultrasonic intensity and frequency, the optical tweezers arrays will act as the dynamical one, which can sieve the beads in (X,Y) plane in the embedding fluid. The focal length of microlens in Germanium controling by frequency and intensity of ultrasonic wave is discussed.&nbsp

Acousto-optical Scanning-Based High-Speed 3D Two-Photon Imaging In Vivo.

Recording of the concerted activity of neuronal assemblies and the dendritic and axonal signal integration of downstream neurons pose different challenges, preferably a single recording system should perform both operations. We present a three-dimensional (3D), high-resolution, fast, acousto-optic two-photon microscope with random-access and continuous trajectory scanning modes reaching a cubic millimeter scan range (now over 950 × 950 × 3000 μm3) which can be adapted to imaging different spatial scales. The resolution of the system allows simultaneous functional measurements in many fine neuronal processes, even in dendritic spines within a central core (>290 × 290 × 200 μm3) of the total scanned volume. Furthermore, the PSF size remained sufficiently low (PSFx < 1.9 μm, PSFz < 7.9 μm) to target individual neuronal somata in the whole scanning volume for simultaneous measurement of activity from hundreds of cells. The system contains new design concepts: it allows the acoustic frequency chirps in the deflectors to be adjusted dynamically to compensate for astigmatism and optical errors; it physically separates the z-dimension focusing and lateral scanning functions to optimize the lateral AO scanning range; it involves a custom angular compensation unit to diminish off-axis angular dispersion introduced by the AO deflectors, and it uses a high-NA, wide-field objective and high-bandwidth custom AO deflectors with large apertures. We demonstrate the use of the microscope at different spatial scales by first showing 3D optical recordings of action potential back propagation and dendritic Ca2+ spike forward propagation in long dendritic segments in vitro, at near-microsecond temporal resolution. Second, using the same microscope we show volumetric random-access Ca2+ imaging of spontaneous and visual stimulation-evoked activity from hundreds of cortical neurons in the visual cortex in vivo. The selection of active neurons in a volume that respond to a given stimulus was aided by the real-time data analysis and the 3D interactive visualization accelerated selection of regions of interest

Finite-Temperature Collective Dynamics of a Fermi Gas in the BEC-BCS Crossover

We report on experimental studies on the collective behavior of a strongly interacting Fermi gas with tunable interactions and variable temperature. A scissors mode excitation in an elliptical trap is used to characterize the dynamics of the quantum gas in terms of hydrodynamic or near-collisionless behavior. We obtain a crossover phase diagram for collisional properties, showing a large region where a non-superfluid strongly interacting gas shows hydrodynamic behavior. In a narrow interaction regime on the BCS side of the crossover, we find a novel temperature-dependent damping peak, suggesting a relation to the superfluid phase transition

High-Throughput Nonlinear Optical Microscopy

High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be important for studying fast kinetic processes, for enabling clinical diagnosis and treatment, and for extending the field of image informatics. This review will provide an overview of the fundamental limitations on NLO microscopy throughput. We will further cover several important classes of high-throughput NLO microscope designs with discussions on their strengths and weaknesses and their key biomedical applications. Finally, this review will close with a perspective of potential future technological improvements in this field.National Institutes of Health (U.S.) (9P41EB015871-26A1)National Institutes of Health (U.S.) (R01-EX017656)National Institutes of Health (U.S.) (5 R01 NS051320)National Institutes of Health (U.S.) (4R44EB012415-02)National Science Foundation (U.S.) (CBET-0939511)Singapore-MIT AllianceSkolkovo Institute of Science and TechnologySingapore. National Research Foundation (Singapore-MIT Alliance for Research and Technology)Wellcome Trust (London, England) (Massachusetts Institute of Technology. Postdoctoral Fellowship 093831/Z/10/Z

(3+1) Massive Dirac Fermions with Ultracold Atoms in Optical Lattices

We propose the experimental realization of (3+1) relativistic Dirac fermions using ultracold atoms in a rotating optical lattice or, alternatively, in a synthetic magnetic field. This approach has the advantage to give mass to the Dirac fermions by coupling the ultracold atoms to a Bragg pulse. A dimensional crossover from (3+1) to (2+1) Dirac fermions can be obtained by varying the anisotropy of the lattice. We also discuss under which conditions the interatomic potentials give rise to relativistically invariant interactions among the Dirac fermions

Optogenetics design of mechanistically-based stimulation patterns for cardiac defibrillation

Current rescue therapies for life-threatening arrhythmias ignore the pathological electro-anatomical substrate and base their efficacy on a generalized electrical discharge. Here, we developed an all-optical platform to examine less invasive defibrillation strategies. An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts. The macroscope was implemented with a random-access scanning head capable of drawing arbitrarily-chosen stimulation patterns with sub-millisecond temporal resolution allowing precise epicardial activation of Channelrhodopsin2 (ChR2). We employed this optical system in the setting of ventricular tachycardia to optimize mechanistic, multi-barrier cardioversion/defibrillation patterns. Multiple regions of conduction block were created with a very high cardioversion efficiency but with lower energy requirements as compared to whole ventricle interventions to interrupt arrhythmias. This work demonstrates that defibrillation energies can be substantially reduced by applying discrete stimulation patterns and promotes the progress of current anti-arrhythmic strategies