Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen

Three biocontrol agent viz., Trichoderma viride, Trichoderma virens and Trichoderma harzianum were evaluated to test the antagonism against Fusarium udum under in vitro conditions. All the three biocontrol agents have the potential of parasitizing the growth of Fusarium udum in vitro. The rate of parasitism was found fastest in T. viride (61.12% over growth in 96 hrs) than T. virens and T. harzianum. The volatile compounds from Trichoderma viride suppressed the mycelial growth of Fusarium udum by 43.13% and found effective when compared to Tricho-derma virens and Trichoderma harzianum. Non-volatile compounds or culture filtrate from Trichoderma virens at 15% concentration shows complete mycelial inhibition of the test fungi. The antagonist T. virens was chosen to be the most promising bio-control agent for F. udum

Control of common scab without the use of water

The most effective way to control common scab is by irrigating a potato crop at tuber initiation. With the introduction of legislation such as the Water Framework Directive this will become increasingly difficult. In this field experiment, we assessed the potential of a number of non-water measures for controlling this disease. Common scab on daughter tubers at harvest was reduced by applying rapeseed meal at 1 t ha-1 to the beds and then incorporating it into the soil, and adding a mixture of Trichoderma viride isolates into the furrow at planting. None of these treatments was as effective as using water

Organic Farming Technologies and Practices Followed by the Farmers

The study mainly focused on organic farming technologies and practices followed by the farmers for coconut, millets, sugarcane and turmeric. The study was conducted in Coimbatore, Erode and Dharampuri districts of Tamil Nadu. A sample of 100 organic farmers was the respondents for the present study. The data were collected from each respondent through personal interview method with the help of interview schedule. The data were analyzed using percentage analysis. The results revealed that, majority of the respondents followed seed treatment with Azospirillum, beejamrutha and panchakavya, application of jeevamruthum and panchakavya through drip irrigation, application of farm yard manure and insitu ploughing of green manure crops, mulching with crop leaf residues and weeds, spraying of agni astram to repel pests, post harvest technology like oil extraction and jaggary preparation, storage techniques like using neem and pungam leaves. The result of the present study shows that, the farmers have the inclination for adoption of organic production practices. Hence it may be concluded from the study that, there is an imperative need to raise the level of adoption of these organic farming practices in order to reduce the quantum of environmental hazards by inorganic farming

Enantiomeric oxidation of organic sulfides by the filamentous fungi Botrytis cinerea, Eutypa lata and Trichoderma viride

The biotransformations of a series of substituted sulfides were carried out with the filamentous fungi Botrytis cinerea, Eutypa lata and Trichoderma viride. Several products underwent microbial oxidation of sulfide to sulfoxide with medium to high enantiomeric purity. With regard to sulfoxide enantioselectivity, the (R)-enantiomer was favoured in biotransformations by T. viride and E. lata while the (S)-enantiomer was favoured in those by B. cinerea. A minor amount of sulfone product was also obtained

Effect of some biocontrol agents against root-knot nematode (Meloidogyne incognita race2)

Culture filtrate of four rhizospheric fungi and four biocontrol agents were studied in vitro for their efficacy against Meloidogyne incognita race 2. The per cent mortality and egg hatching inhibition was proportional to the concentration of culture filtrate and the duration of exposure period. Culture filtrates of Trichoderma viride, Trichoderma harzianum, Trichoderma sp., Fusarium sp., Penicillium sp. and Aspergillus sp. significantly induced inhibition of egg hatching and mortality of Meloidogyne incognita race 2.The highest percentage of inhibition of egg hatching and juvenile mortality was recorded in Trichoderma harzianum followed by Trichoderma viride and Trichoderma sp

Susceptibility of Several Northeastern Conifers to Fusarium circinatum and Strategies for Biocontrol

Producción CientíficaFusarium circinatum, the causal of pine pitch canker disease (PPC), is now considered among the most important pathogens of Pinaceae in the world. Although in Europe PPC is only established in the Iberian Peninsula, the potential endangered areas cover over 10 million hectares under the current host distribution and climatic conditions. It is therefore a priority to test the susceptibility of those species and their provenances, within Central and Northern Europe and find biological control agents (BCAs) against the disease. In this study, the susceptibility of Pinus sylvestris, P. mugo and Picea abies Romanian provenances to F. circinatum was tested using three inoculum doses. In parallel, the potential use of Trichoderma atroviride and Trichoderma viride as BCAs against F. circinatum was also tested. This study has demonstrated, for the first time, the susceptibility of P. mugo to F. circinatum. Likewise, the susceptibility of P. abies was also confirmed. The fact that the Romanian provenance of P. sylvestris has not been susceptible to F. circinatum suggests genetic resistance as a potential tool to manage the disease. This, together with the apparent effectiveness of Trichoderma species as BCAs, seems to indicate that an integrated management of the disease might be feasible.European Cooperation in Science and Technology (COST Action FP1406 PINESTRENGTH)Ministerio de Economía, Industria y Competitividad (projects AGL2012-39912 / AGL2015-69370-R)Portuguese Foundation for Science and Technology (grant SFRH/BPD/122928/2016

Biocontrol of leucoagaricus gongylophorus of leaf-cutting ants with the mycoparasitic agent trichoderma koningiopsis

Leaf-cutting ants are one of the main agricultural and agroforestry pests in the Neotropic region. The essential food source of these ants is Leucoagaricus gongylophorus. Therefore one of the main biocontrol agents under study are Trichoderma species, because of their biocontrol activity against a diverse range of fungi. Here, Trichoderma koningiopsis, isolated from a leaf-cutting ants nest was tested against three Leucoagaricus gongylophorus strains from leaf-cutting ants by dual culture technique under laboratory conditions. The molecular analysis of ITS sequence data showed three well-separated main clades in which the isolated Trichoderma strain was assembled as a sole subclade among T. koningiopsis strains. The tests also showed that T. koningiopsis strain inhibited the growth of all L. gongylophorus strains tested. The values of radial inhibition of L. gongylophorus ranged from 58% to 69% with an average mean value of 65%. This is the first report on a strain of T. koningiopsis isolated from a naturally parasitized nest of leaf-cutting ants with biocontrol ability over L. gongylophorus tested in dual culturing method.Fil: Castrillo, María Lorena. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bich, Gustavo Angel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Zapata, Pedro Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; ArgentinaFil: Villalba, Laura. Universidad Nacional de Misiones. Facultad de Cs.exactas Quimicas y Naturales. Departamento de Bioquimica Clinica. Laboratorio de Biotecnologia Molecular; Argentin

Spesies Unggul Trichoderma Spp Indigenus Rizozfir Pisang sebagai Pengendali Penyakit Layu Fussarium pada Bibit Tanaman Pisang Mas Hasil Kultur In Vitro

This research will be attempts to obtain strain superior Trichoderma that had ability to colonize roots in mas cultivar banana seedlings so that expected can induce resistant plants to the attack fungi Fusarium oxysporum f.sp . the cause of the disease wither in banana crop result in vitro cultures.Research carried out in the Green House Agricultural Faculty, University of Muhammadiyah Purwokerto, time or carried out 8 months. A design that is a Random Block Design treatment which were examined consists of 2 factor of treatment that is for biological agent antagonist Trichoderma sp, consisting of 2 species that is Trichorderma harzianum (T1), and Trichorderma viride (T2 processor). The second treatment is a way to Trichoderma application that consists of two that is, by immersion banana into the suspension Trichoderma (P1) and the sprinkling planting medium to isolate Trichoderma spp (P2). All organised in factorial with three replications, and each unit treatment uses 10 plants that will use 40 polybags. The result showed that application biological agent Trichoderma (T. Harzianum and T viride) during the research proved an emphasis on the attack disease Fussarium in seed, was shown to the low disease severity attacks. For biologist agenues and biodiversity Trichoderma ( T. Harzianum and T. Viride) which is applied by immersion and the sprinkling can have an influence on the increase of leaves in seeds during the research, and have no effect on than plants and diameter in each stem treatment. However, it is a gift Trichoderma growth able to give a better than without treatment Trichoderma (control) and proved to be able to colonize banana seedlings root is endofit in banana mas seedling result in vitro culture. Keywords: Fusarium, Trichoderma, Biological control Technolog

Pathogenic fungi and Bio-control agents: Competitive bio-assay research

Fungi of the genus Trichoderma have a track record of being antagonist to quite of a number of agricultural important pathogens. Trichoderma have some unique characteristics that make it scientifically proven and suitable bio-control agents against varieties of pathogenic organism infecting economic food crops. Trichoderma has the advantage of being environment friendly and not hazardous to the health of human beings, livestock, soil and environment. Competitive bio-assay experiment was carried out in the laboratory on the effects of Trichoderma species (T. atroviride P1 isolates, T. harzianum T22 isolates, T. viride) on some crop pathogens (Phytophthora cinnanerium, Botrytis cinaria and Rhizoctonia solani). Pure culture of Trichoderma and pathogenic fungi were replicated four times and arranged in a complete block design. The result of the experiment shows that Trichoderma species are strong competitor of P. cinnanerium, B. cinaria and R.solani. Within 72 hours, the Trichoderma species were able to grow and completely overlap the P. cinnanerium, B. cinaria and R. solani. This strong competitiveness indicated that Trichoderma species would effectively inhibit the growth of P. cinnanerium, B. cinaria and R. solani on the infected crop; thus the application of Trichoderma species in the control of P. cinnanerium, B. cinaria and R. solani infected crops