Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach

Regarding future space exploration missions and long-term exposure experiments, a detailed investigation of all factors present in the outer space environment and their effects on organisms of all life kingdoms is advantageous. Influenced by the multiple factors of outer space, the extremophilic bacterium Deinococcus radiodurans has been long-termly exposed outside the international Space Station in frames of the tanpopo orbital mission. the study presented here aims to elucidate molecular key components in D. radiodurans, which are responsible for recognition and adaptation to simulated microgravity. D. radiodurans cultures were grown for two days on plates in a fast-rotating 2-D clinostat to minimize sedimentation, thus simulating reduced gravity conditions. Subsequently, metabolites and proteins were extracted and measured with mass spectrometry-based techniques. our results emphasize the importance of certain signal transducer proteins, which showed higher abundances in cells grown under reduced gravity. these proteins activate a cellular signal cascade, which leads to differences in gene expressions. Proteins involved in stress response, repair mechanisms and proteins connected to the extracellular milieu and the cell envelope showed an increased abundance under simulated microgravity. focusing on the expression of these proteins might present a strategy of cells to adapt to microgravity conditions

Creative Improvisation in Disaster Responses : Practice in areas aff ected by the Kumamoto earthquakes

In the face of disasters, organizations and groups collaborate continuously and fluidly, coordinating with one another in order to overcome extraordinary challenges. When researchers explored this phenomenon in-depth, they employed the concept of “improvisation.” However, there is a dearth of research in the area of “creative improvisation” relating to civic agencies that do not have special plans or approaches to disaster response. This study, therefor, aims at identifying some of the most eff ective improvisational approaches taken by civic agencies in the fi eld of communication where information, consciousness, and ideas converge. This paper classifi es cases of creative improvisation observed during the April 2016 Kumamoto earthquakes into two types and considers the respective conditions of improvisation. These are: 1) “Place-making”: by raising several cases representing “place to convergence” scattered throughout the region, the improvisational condition that can be summarized as, "Awareness of gathering to support the aff ected key places" is highlighted. 2) “Forming a commonality”: through participant observation at the "informal talk" held by local civil agencies, the interaction between agencies in the sense-making process is highlighted, thus clarifying the improvisational approaches of, "the process of realizing the role while being encouraged to participate initially," and "ingenuity to induce new movement by co-editing opposite opinions"

Construyendo redes de amistad y espacios interculturales: el caso de las mujeres japonesas en España

This study examines the associationism of Japanese women living in Spain who are members of the Tanpopo-no-kai/Sociedad Tanpopo, and their intercultural experiences through their written personal narratives. This associationism has resulted in attempts to create a social network and space for intercultural communication at a national level between Hispanic-Japanese families and Japanese residents in Spain. The research is based on numerical data and narrative data, however Japanese narratives from the quarterly association bulletins provide the main source of information. By using the narrative analysis method in the form of questions we have been able to study the writings in order to explore issues such as: their transcultural experiences, their processes of adapting to life in Spain, the evolution of their identity, their interpretation of the social reality around them and the passing on of their social and cultural heritage to their children, among others. The study of the Tanpopo association has demonstrated a close-knit and relaxed transcultural and female space for intraethnic communication. It reveals a joint strategy of emotional and instrumental support to overcome the isolation, the loss of social networks and the geographical separation from their own cultural region, resulting from the immigration through marriage of Japanese women living in Spain.U radu se istražuje udruživanje japanskih žena koje žive u Španjolskoj i članice su Društva Tanpopo (Tanpopo-no-kai/Sociedad Tanpopo) te njihova interkulturna iskustva kroz osobne pisane priče. Iz tog su udruživanja proizašli pokušaji stvaranja društvene mreže i prostora za interkulturnu komunikaciju na državnoj razini između španjolsko-japanskih obitelji i Japanaca koji borave u Španjolskoj. Istraživanje se temelji na brojčanim i narativnim podacima; glavni su izvor informacija pripovijesti Japanaca u tromjesečnom biltenu Društva. Primjena metode narativne analize u obliku pitanja omogućila je proučavanje napisanoga kako bi se istražila, između ostalih, sljedeća pitanja: njihova transkulturna iskustva, procesi prilagodbe životu u Španjolskoj, razvoj njihova identiteta, njihova interpretacija društvene stvarnosti koja ih okružuje i prenošenje njihova društvenoga i kulturnog naslijeđa na djecu. Proučavanje Društva Tanpopo pokazalo je da postoji čvrsto povezan i opuštajući transkulturni i ženski prostor za unutaretničku komunikaciju. Ono otkriva zajedničku strategiju emocionalne i instrumentalne potpore za prevladavanje osamljenosti, gubitka društvenih mreža i geografske odvojenosti od njihove kulturne okoline kao posljedice imigracije putem udaje japanskih žena koje žive u Španjolskoj.Este estudio examina el asociacionismo de las mujeres japonesas residentes en España y miem¬bros de la Tanpopo-no-kai/Sociedad Tanpopo, y sus experiencias interculturales a través de sus narrativas escritas. Este asociacionismo ha intentado crear una red de amistad y un espacio comunicativo intercultural a nivel estatal, entre familias hispano-japonesas y japoneses residentes en España. Esta investigación se basa en datos numéricos y narrativos, aunque la fuente de datos principal han sido las narrativas de sus boletines trimestrales. Con la utilización del método de análisis de narrativas en forma de preguntas, se han podido analizar sus escritos para explorar temas como: sus experiencias transculturales, sus procesos de adaptación a España, la evolución de su identidad, su interpretación de la realidad social que las envuelve, la transmisión de su herencia social y cultural japonesa a los hijos, entre otras. El estudio de la Sociedad Tanpopo nos muestra un espacio femenino transcultural íntimo y relajado para la comunicación intraétnica, y nos desvela una estrategia conjunta de apoyo emocional e instrumental para superar el aislamiento, la pérdida de redes sociales y la separación geográfica de la propia región cultural, que han acompañado a la inmigración por matrimonio de las mujeres japonesas residentes en España

Taraxacum

Fil: Hurrell, Julio Alberto. Laboratorio de Etnobotánica y Botánica Aplicada (LEBA). Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; ArgentinaFil: Delucchi, Gustavo. División Plantas Vasculares. Facultad de Ciencias Naturales y Museo. Universidad Nacional de La Plata; Argentin