Characterisation of the relationship between surface texture and surface integrity of superalloy components machined by grinding

The surface texture of a machined component is influenced largely by the processing parameters used during machining and hence, there is a relationship between both the formation of the surface texture and surface integrity of the machined component. In the study to be reported in this paper, GH4169, a hard-to-cut superalloy, widely used in aero-engines, was selected for a detailed investigation into the relationship between the surface texture and the component-performance (surface integrity) of the machined components for which a series of grinding experiments with different grinding-wheels and grinding parameter-values was carried out in order to quantitatively analyze variations of the surface roughness with processing parameters. Further, considering that the features of the ground-surfaces measured are of a random nature, statistic properties of the produced surfaces were revealed and characterised with power spectral density function (PSD) and auto-covariance function(ACV) method respectively

Links Between Machining Parameters and Surface Integrity in Drilling Ni-Superalloy

In aerospace industry, the manufacturing of critical parts (high energy components) requires an important validation process to guarantee the quality of the produced parts, and thus their fatigue lifecycle. Globally, this validation consists in freezing the cutting conditions using metallurgical analysis or fatigue trials, and a test on the first article. This process is extremely complex and expensive. In this way establishing the correlation between the cutting conditions and the surface integrity will help us to optimize the manufacture of those parts. In this article, by the means of an experimental method, we define a domain of validation by combining the cutting conditions according to the classic criteria established by AFNOR E66-520 norm (Couple-Tool-Material) and the criteria of surface integrity for the drilling of a Nickel-base superalloy. The experimental device consists in drilling a Ø15.5 mm hole on a 3-axis milling centre instrumented by a 4 components Kistler dynamometer (Fx, Fy, Fz and Mz), a spindle power sensor “Watt-pilote” and three accelerometers placed following the directions X, Y and Z. Scanning Electron Microscopy (SEM) observations, micro-hardness tests and topographic measurements with an optical profilometer, are carried out to characterize the metallurgical state of the holes manufactured. Finally, correlations were respectively made between the cutting conditions, the recorded signals and the metallurgical state of the holes

Characterization of the Adherence of Clostridium difficile Spores: The Integrity of the Outermost Layer Affects Adherence Properties of Spores of the Epidemic Strain R20291 to Components of the Intestinal Mucosa

Indexación: Web of Science.Clostridium difficile is the causative agent of the most frequently reported nosocomial diarrhea worldwide. The high incidence of recurrent infection is the main clinical challenge of C. difficile infections (CBI). Formation of C. difficile spores of the epidemic strain R20291 has been shown to be essential for recurrent infection and transmission of the disease in a mouse model. However, the underlying mechanisms of how these spores persist in the colonic environment remains unclear. In this work, we characterized the adherence properties of epidemic R20291 spores to components of the intestinal mucosa, and we assessed the role of the exosporium integrity in the adherence properties by using cdeC mutant spores with a defective exosporium layer. Our results showed that spores and vegetative cells of the epidemic R20291 strain adhered at high levels to monolayers of Caco-2 cells and mucin. Transmission electron micrographs of Caco-2 cells demonstrated that the hair-like projections on the surface of R20291 spores are in close proximity with the plasma membrane and microvilli of undifferentiated and differentiated monolayers of Caco-2 cells. Competitive-binding assay in differentiated Caco-2 cells suggests that spore-adherence is mediated by specific binding sites. By using spores of a cdeC mutant we demonstrated that the integrity of the exosporium layer determines the affinity of adherence of C. difficile spores to Caco-2 cells and mucin. Binding of fibronectin and vitronectin to the spore surface was concentration-dependent, and depending on the concentration, spore-adherence to Caco-2 cells was enhanced. In the presence of an aberrantly-assembled exosporium (cdeC spores), binding of fibronectin, but not vitronectin, was increased. Notably, independent of the exosporium integrity, only a fraction of the spores had fibronectin and vitronectin molecules binding to their surface. Collectively, these results demonstrate that the integrity of the exosporium layer of strain R20291 contributes to selective spore adherence to components of the intestinal mucosa.http://journal.frontiersin.org/article/10.3389/fcimb.2016.00099/ful

An ultra scale-down analysis of the recovery by dead-end centrifugation of human cells for therapy.

An ultra scale-down method is described to determine the response of cells to recovery by dead-end (batch) centrifugation under commercially defined manufacturing conditions. The key variables studied are the cell suspension hold time prior to centrifugation, the relative centrifugal force (RCF), time of centrifugation, cell pellet resuspension velocities, and number of resuspension passes. The cell critical quality attributes studied are the cell membrane integrity and the presence of selected surface markers. Greater hold times and higher RCF values for longer spin times all led to the increased loss of cell membrane integrity. However, this loss was found to occur during intense cell resuspension rather than the preceding centrifugation stage. Controlled resuspension at low stress conditions below a possible critical stress point led to essentially complete cell recovery even at conditions of extreme centrifugation (e.g., RCF of 10000 g for 30 mins) and long (~2 h) holding times before centrifugation. The susceptibility to cell loss during resuspension under conditions of high stress depended on cell type and the age of cells before centrifugation and the level of matrix crosslinking within the cell pellet as determined by the presence of detachment enzymes or possibly the nature of the resuspension medium. Changes in cell surface markers were significant in some cases but to a lower extent than loss of cell membrane integrity. Biotechnol. Bioeng. 2015;112: 997-1011. © 2014 Wiley Periodicals, Inc

Chaining of welding and finish turning simulations for austenitic stainless steel components

The chaining of manufacturing processes is a major issue for industrials who want to understand and control the quality of their products in order to ensure their in-service integrity (surface integrity, residual stresses, microstructure, metallurgical changes, distortions,…). Historically, welding and machining are among the most studied processes and dedicated approaches of simulation have been developed to provide reliable and relevant results in an industrial context with safety requirements. As the simulation of these two processes seems to be at an operationnal level, the virtual chaining of both must now be applied with a lifetime prediction prospect. This paper will first present a robust method to simulate multipass welding processes that has been validated through an international round robin. Then the dedicated “hybrid method”, specifically set up to simulate finish turning, will be subsequently applied to the welding simulation so as to reproduce the final state of the pipe manufacturing and its interaction with previous operations. Final residual stress fields will be presented and compared to intermediary results obtained after welding. The influence of each step on the final results will be highlighted regarding surface integrity and finally ongoing validation works and numerical modeling enhancements will be discussed

Surface Modification of Biodegradable Porous Mg Bone Scaffold Using Polycaprolactone/Bioactive Glass Composite

A reduction in the degradation rate of magnesium (Mg) and its alloys is in high demand to enable these materials to be used in orthopedic applications. For this purpose, in this paper, a biocompatible polymeric layer reinforced with a bioactive ceramic made of polycaprolactone (PCL) and bioactive glass (BG) was applied on the surface of Mg scaffolds using dip-coating technique under low vacuum. The results indicated that the PCL-BG coated Mg scaffolds exhibited noticeably enhanced bioactivity compared to the uncoated scaffold. Moreover, the mechanical integrity of the Mg scaffolds was improved using the PCL-BG coating on the surface. The stable barrier property of the coatings effectively delayed the degradation activity of Mg scaffold substrates. Moreover, the coatings induced the formation of apatite layer on their surface after immersion in the SBF, which can enhance the biological bone in-growth and block the microcracks and pore channels in the coatings, thus prolonging their protective effect. Furthermore, it was shown that a three times increase in the concentration of PCL-BG noticeably improved the characteristics of scaffolds including their degradation resistance and mechanical stability. Since bioactivity, degradation resistance and mechanical integrity of a bone substitute are the key factors for repairing and healing fractured bones, we suggest that PCL-BG is a suitable coating material for surface modification of Mg scaffolds

Residual Stresses Induced by Dry and Cryogenic Cooling during Machining of AZ31B Magnesium Alloy

The major challenge of the Mg alloys has been their unsatisfactory corrosion resistance, which can be enhanced by improving the surface integrity. Cryogenic machining, where liquid nitrogen was used during machining, has been reported to improve the surface integrity of machined components, including compressive residual stresses. This paper analyses the influence of several cutting parameters, tool geometry and cryogenic conditions on the surface and subsurface residual stresses distribution

Characterising the impact of surface integrity on the fatigue behaviour of a shot-peened connecting rod

The present study focuses on analysing and modelling the influence on fatigue behaviour of the surface of a hot-forged C70 connecting rod which undergoes a shot-blasting treatment. The shot-blasting heavily affects the surface and thus the fatigue properties. In addition, the forging process introduces large defects which also have an effect on the fatigue strength. So as to be able to determine which aspects of the surface integrity are the most influential in fatigue, additional surface states were generated by shot-peening the as-forged surface. The various surface states were thoroughly characterised and then tested in high cycle fatigue in bending. The various aspects studied are the surface roughness and large defects, residual stresses, and microstructure