Multi-scale structure, pasting and digestibility of adlay (Coixlachryma-jobi L.) seed starch

peer-reviewedThe hierarchical structure, pasting and digestibility of adlay seed starch (ASS) were investigated compared with maize starch (MS) and potato starch (PS). ASS exhibited round or polyglonal morphology with apparent pores/channels on the surface. It had a lower amylose content, a looser and more heterogeneous C-type crystalline structure, a higher crystallinity, and a thinner crystalline lamellae. Accordingly, ASS showed a higher slowly digestible starch content combined with less resistant starch fractions, and a decreased pasting temperature, a weakened tendency to retrogradation and an increased pasting stability compared with those of MS and PS. The ASS structure-functionality relationship indicated that the amylose content, double helical orders, crystalline lamellar structure, and surface pinholes should be responsible for ASS specific functionalities including pasting behaviors and in vitro digestibility. ASS showed potential applications in health-promoting foods which required low rearrangement during storage and sustainable energy-providing starch fractions

Nutritional characterization of gluten free non-traditional pasta

When a food is formulated, its characterization is important from the chemical and biochemical point of view; even more whennon-traditional raw materials are used. Noodles were made with cassava starch and corn flour (4:1), milk, egg, salt and xanthangum. The chemical composition of the pasta was determined and the total and resistant starch content was quantified. Thehydrolysis rate of the starch was measured at different times, from which the hydrolysis index and, subsequently, the predictiveglycemic index was calculated. The chemical composition of the noodles showed its high content of total fibers. From thedigestibility tests, high values were obtained for proteins (93%), and average values for the starch (52%). The results of the starchhydrolysis kinetics showed a higher proportion of slowly digestible starch with a low glycemic index (46%). Analyzed noodles arewithin the dietary guidelines that suggest a diet with high total dietary fiber content and low glycemic index.Fil: Milde, Laura Beatríz. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Chigal, Paola Soledad. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Chiola Zayas, Maria Ofelia. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales; Argentin

Cereal non-starch polysaccharides in pig diets

This thesis is based on three different studies comprising the weaning and the growing period, aiming at monitoring the influence of cereal non-starch polysaccharides (NSP) and dietary enzyme supplementation on gastro-intestinal processes in pigs. The diets were based on cereals and cereal by-products, and composed to contain different amounts of total as well as soluble NSP. Results from these studies have shown that with increased NSP content, the reduction in digestibility of organic matter (OM) was twice as high in the small intestine than in the total tract, both in newly weaned piglets and growing pigs. An increased proportion of insoluble NSP decreased the digestibility of OM and fibre components in the small intestine of the newly weaned piglets and in the total tract of growing pigs. The gut environment, as described by content and proportions of organic acids (OA) and pH, as well as total microbial populations and coliform diversity, was altered by NSP content and solubility, whereas enzyme supplementation influenced the distribution of OA in the small intestine. PVTC-cannulation did not influence the coliform flora, and results obtained from PVTC-cannulated pigs were concluded to reflect true intestinal conditions. In conclusion, these results indicate that the dietary content of total and soluble NSP influence gastro-intestinal processes such as digestion site, gut environment and microbial populations in different ways in newly weaned piglets and in growing pigs. Therefore, NSP constitute an important tool with possibilities to influence gut health in pigs, and may therefore offer prospects to optimise the feed for pigs of different age

Factors affecting sorghum protein digestibility

In the semi-arid tropics worldwide, sorghum (Sorghum bicolor (L.) Moench) is cultivated by farmers on a subsistence level and consumed as food by humans. A nutritional limitation to its use is the poor digestibility of sorghum protein when wet cooked. The factors affecting wet cooked sorghum protein digestibility may be categorised into two main groups: exogenous factors (grain organisational structure, polyphenols, phytic acid, starch and non-starch polysaccharides) and endogenous factors (disulphide and non-disulphide crosslinking, kafirin hydrophobicity and changes in protein secondary structure). All these factors have been shown to influence sorghum protein digestibility. More than one factor may be at play at any time depending on the nature or the state in which the sorghum grain is; that is whether whole grain, endosperm, protein body preparation, high-tannin or condensed-tannin-free. It is proposed that protein crosslinking may be the greatest factor that influences sorghum protein digestibility. This may be between ?- and ß-kafirin proteins at the protein body periphery, which may impede digestion of the centrally located major storage protein, a-kafirin, or between ?- or ß-kafirin and a-kafiri

Effects of replacing different proportions of barley grain by barley fibre on performance of dairy bulls

The objective of the present experiment was to study the effects of partial replacement of barley grain with barley fibre (BF) on animal performance, carcass traits and diet digestibility of growing dairy bulls. The feeding experiment comprised 20 Finnish Ayrshire bulls and 12 Holstein-Friesian bulls, and four treatments (8 bulls per treatment). There were four diets with two offered at stage 1 (from the initiation of the study to 450 kg live weight) and four at stage 2 (from 450 kg live weight to slaughter). The control diet (BF0) included grass silage (460 g kg–1 dry matter) and barley grain (540), BF25 diet included grass silage (460), barley grain (405) and BF (135), BF50 diet included grass silage (460), barley grain (270) and BF (270), and BF75 diet included grass silage (460), barley grain (135) and BF (405). At stage 1 there were only two treatments (BF0 and BF50) and at stage 2, all four treatments were included. All bulls were fed total mixed ration ad libitum. The mean initial live weight of the bulls was 261 kg and the mean final live weight 650 kg. At stage 1 there were no significant treatment differences in dry matter, energy or protein intakes or in live weight gain. At stage 2, replacing barley grain with BF led to a linear decrease of daily live weight gain (P < 0.05) and a linearly reduced feed conversion (kg dry matter kg–1 live weight gain) (P < 0.05). The apparent digestibility of the organic matter and neutral detergent fibre decreased linearly with increasing BF supplementation (P < 0.001). The dressing proportion and the carcass fat score decreased linearly (P < 0.05) with partial replacement of barley grain with BF. On carcass conformation, treatment had a significant (P < 0.05) quadratic effect: the BF25 and BF50 diets were classified highest. The results indicate that 50% of barley starch can be replaced with BF without affecting growth, but feed efficiency factors may decrease when barley starch is replaced with BF. At 75% replacement, feed intake was reduced, which resulted in a lower energy intake and reduced level of performance

Plant cell walls: impact on nutrient bioaccessibility and digestibility

Cell walls are important structural components of plants, affecting both the bioaccessibility and subsequent digestibility of the nutrients that plant-based foods contain. These supramolecular structures are composed of complex heterogeneous networks primarily consisting of cellulose, and hemicellulosic and pectic polysaccharides. The composition and organization of these different polysaccharides vary depending on the type of plant tissue, imparting them with specific physicochemical properties. These properties dictate how the cell walls behave in the human gastrointestinal tract, and how amenable they are to digestion, thereby modulating nutrient release from the plant tissue. This short narrative review presents an overview of our current knowledge on cell walls and how they impact nutrient bioaccessibility and digestibility. Some of the most relevant methods currently used to characterize the food matrix and the cell walls are also described

Effects of Starch and Fat Concentrations in Starter Grain on Jersey Calf Performance

We investigated the nutritional needs of Jersey calves, focusing on meeting energy requirements by altering starch and fat concentrations in calf starters. Thirty-six female Jersey calves were grouped by BW and birth date and randomly assigned among 3 calf starters: 35% starch and 2% fat (HST), 20% starch and 2% fat (LST), and 35% starch and 4% fat (HST-F). The fat supplement for HST-F consisted of 20% coconut oil, 45% lard, 15% flaxseed oil, and 20% soybean oil. Calves were fed 4 L of colostrum at birth. All calves were fed the same milk replacer at 4.4 L/d during week one, 5.2 L/d during wk 2 to 7, and 2.6 L/d during wk 8 prior to weaning. Intake was measured daily and wither heights (WH) and BW were measured weekly. One week after weaning, fecal and feed samples were collected daily for 3 d. Using acid insoluble ash, apparent digestibility of dry matter (DM), neutral detergent fiber (NDF), and crude protein (CP) were determined. Neither starter (0.54, 0.52, and 0.56 kg/d, respectively for LST, HST, and HST-F) nor total DM intakes (1.05, 0.99, and 1.08 kg/d, respectively) differed among treatments. Average daily gains from birth through wk 10 were similar among groups (0.534, 0.586, and 0.550 kg/d, respectively), thus BW and WH were similar across weeks. Digestibilities of DM (66.0, 73.4, and 70.2%, respectively), NDF (50.2, 41.6, and 41.4%, respectively), and CP (65.1, 67.6, and 62.6%, respectively) were similar among treatments. Fecal scoring was evaluated on a 1-6 scale. For the first 4 weeks, there were no differences among treatments for average fecal scores and days with fecal scores of 1 to 4. Starch and fat concentrations in the starters appeared to not affect calf performance which can provide flexibility in formulation of starters based on ingredient costs.AJCC Research Foundation of the American Jersey Cattle AssociationHonors Committee for OSU's College of Food, Agricultural, and Environmental SciencesNo embargoAcademic Major: Animal Science

Effects of forage supplements on milk production and chemical properties, in vivo digestibility, rumen fermentation and N excretion in dairy cows offered red clover silage and corn silage or dry ground corn

peer-reviewedThis study concerned the effects of partial substitution of clover silage with high starch forages on milk production and chemical composition, in vivo digestibility, rumen fermentation pattern and nitrogen excretion of dairy cows. Sixteen dairy cows were separated into two groups and were assigned to treatments in a two-period crossover design. Two forage supplements were used: corn silage (CS) and dry ground corn (DG). All animals received 4.5 kg of concentrate dry matter per day. Results showed no significant difference between the forage supplements for milk production, while significant differences (P<0.01) were observed for milk fat, milk protein and nitrogen utilisation efficiency (42 v. 4.0 g/kg, 3.5 v. 3.3 g/kg and 222 v. 188 g/kg, respectively, for DG and CS). Faecal N excretion did not differ between forage supplements, but urinary N excretion was higher for CS (P<0.05). No significant differences were observed between treatments for rumen fluid pH or for rumen fluid concentrations of ammonium nitrogen or of acetic, propionic or butyric acids. Dry matter intake and the in vivo digestibility of dry matter, organic matter, acid detergent fibre and neutral detergent fibre were all higher for CS compared with DG.The authors are grateful to the Environmental Office of the Cantabrian Government for funding the project 05-640.02-2174

Quantification of nutrient supply in forage-based diets for beef cattle

End of Project ReportIntroduction Cattle rearing systems in Ireland are predominantly grass-based as 80% of agricultural land is dedicated to grassland (silage, hay and pasture) (CSO, 2007). Feed costs represent the largest single variable cost in beef production in Ireland. Grazed grass is generally the cheapest source of food available for beef (and milk) production provided that the environment and management permit high yields of high quality herbage to be utilised (McGee, 2000). Environmental legislation and the rules of environmental schemes such as the European Union (EU) Rural Environmental Protection Scheme are progressively restricting the application of fertilizer Nitrogen (N), and many grazing systems are becoming more extensive. Over 80% of all farms in Ireland make grass silage (O’Kiely et al., 1998) and it accounts for 87% of total grass conserved (Mayne and O’Kiely, 2005). The deficiencies in nutrient supply to beef cattle from grass silage are usually overcome by supplementing with concentrates (McGee, 2005), which are primarily cereal-based (Drennan et al., 2006). However, diverse types of concentrates containing a variety of feed ingredients, particularly non-cereal by-products are available and frequently fed as supplements to grass silage or as highconcentrate diets. The relatively small amount of information available on feeding these contrasting concentrates to beef cattle is inconsistent. Moreover, there has been an increased use of other ensiled forages such as maize and whole-crop cereals. These forages have high intake potential and can reduce the concentrate feeding level, while maintaining or increasing performance of beef cattle (Keady, 2005). With increasing costs of beef production and increasing constraints of environmental regulations, efficient utilisation of consumed nutrients by cattle is imperative in providing sustainable production and income to farmers. Feed evaluation systems are used to match the dietary nutrient supply with animal requirements for a specific level of production (Dijkstra et al., 2007). These systems are important in order to optimise the efficiency of feed utilisation, to improve animal performance and to reduce nutrient losses to the environment (Dijkstra et al., 2007). Although the reticulo-rumen is central to the profile of nutrients available for absorption, yet quantitative knowledge of the rates of passage and the digestion of nutrients in the rumen is limited compared with that on degradation rates (Dijkstra et al., 2007). There is a lack of information that adequately characterises the supply of nutrients from forages and feedstuffs specific to Ireland, especially for fresh grass-based diets of which, there are very few studies reported in the literature. This shortcoming impedes our ability to capitalise on the merits of evolving feeding systems. This project aimed to: 1. Increase the knowledge and advance the understanding on rumen digestion and nutrient flow from the rumen of the main forages / forage-based diets offered to beef cattle in Ireland. 2. Evaluate strategies for optimal utilization of nutrients consumed by cattle

Effect of variety, endosperm hardness, the 1B/1R translocation and enzyme addition on the nutritive value of wheat for growing pigs

It has been widely recognised that wheat chemical composition and nutritive value can vary as a result of genotypic differences, but there is a lack of information on wheat grown in Northern Ireland. Furthermore, there have been conflicting reports regarding the effect of endosperm hardness, the 1B/1R translocation and enzyme addition on the nutritive value of wheat for growing pigs. The effects of wheat variety, endosperm hardness, the presence of the 1B/1R translocation and enzyme addition were examined in four experiments involving a total of 326 Large White Landrace pigs. Performance traits of individually housed pigs were measured in Experiments 1, 3 and 4 and apparent in vivo digestibility coefficients were determined at the total tract and ileal level from post-valve-T-caecum cannulated pigs in Experiment 2. The results obtained for the analysis of the chemical composition of the eight varieties were mainly within reported limits. However, there was a wide range of crude protein concentrations (97.8 to 138.7g/kg dry matter) suggesting varietal differences. There was no effect of endosperm hardness or the 1B/1R translocation on chemical composition. In contrast to other research reports, there were no significant differences in pig performance as a result of either variety, endosperm hardness or the presence of the 1B/1R translocation. However, there were significant variety effects on apparent total-tract digestibility coefficients. Enzyme supplementation had no significant effect on pig performance, despite the fact that the basal diet did not have a high nutrient specification.The authors acknowledge the Department of Agriculture and Rural Development for Northern Ireland (DARD) and Danisco Animal Nutrition for the financial support which enabled this research to be undertaken. Appreciation is expressed to the staff from the Agri-Food and Biosciences Institute who assisted with this work