Skin thickness of the anterior, anteromedial, and anterolateral thigh: a cadaveric study for split-skin graft donor sites

Background: The depth of graft harvest and the residual dermis available for reepithelization primarily influence the healing of split-skin graft donor sites. When the thigh region is chosen, the authors hypothesize based on thickness measurements that the anterolateral region is the optimal donor site. Methods: Full-thickness skin specimens were sampled from the anteromedial, anterior, and anterolateral regions of human cadavers. Skin specimens were cut perpendicularly with a custom-made precision apparatus to avoid the overestimation of thickness measurements. The combined epidermal and dermal thicknesses (overall skin thickness) were measured using a digital calliper. The specimens were histologically stained to visualize their basement membrane, and microscopy images were captured. Since the epidermal thickness varies across the specimen, a stereological method was used to eliminate observer bias. Results: Epidermal thickness represented 2.5% to 9.9% of the overall skin thickness. There was a significant difference in epidermal thickness from one region to another (P<0.05). The anterolateral thigh region had the most consistent and highest mean epidermal thickness (60±3.2 µm). We observed that overall skin thickness increased laterally from the anteromedial region to the anterior and anterolateral regions of the thigh. The overall skin thickness measured 1,032±435 µm in the anteromedial region compared to 1,220±257 µm in the anterolateral region. Conclusions: Based on skin thickness measurements, the anterolateral thigh had the thickest epidermal and dermal layers. We suggest that the anterolateral thigh region is the optimal donor site for split-skin graft harvests from the thigh

Isospin Diffusion in Heavy-Ion Collisions and the Neutron Skin Thickness of Lead

The correlation between the thickness of the neutron skin in Pb-208, and the degree of isospin diffusion in heavy-ion collisions is examined. The same equation of state is used to compute the degree of isospin diffusion in an isospin-depedent transport model and the neutron skin thickness in the Hartree-Fock approximation. We find that skin thicknesses less than 0.15 fm are excluded by the isospin diffusion data.Comment: 5 pages, 4 figures; few minor corrections and updates; version to appear in PR

Nuclear symmetry energy and neutron skin thickness

The relation between the slope of the nuclear symmetry energy at saturation density and the neutron skin thickness is investigated. Constraints on the slope of the symmetry energy are deduced from the neutron skin data obtained in experiments with antiprotonic atoms. Two types of neutron skin are distinguished: the "surface" and the "bulk". A combination of both types forms neutron skin in most of nuclei. A prescription to calculate neutron skin thickness and the slope of symmetry energy parameter $L$ from the parity violating asymmetry measured in the PREX experiment is proposed.Comment: 12 pages, 5 figures, Presented at XXXII Mazurian Lakes Conference on Physics, Piaski, Poland, September 11-18, 201

Probing neutron-skin thickness with total reaction cross sections

We analyze total reaction cross sections, $\sigma_R$, for exploring their sensitivity to the neutron-skin thickness of nuclei. We cover 91 nuclei of O, Ne, Mg, Si, S, Ca, and Ni isotopes. The cross sections are calculated in the Glauber theory using the density distributions obtained with the Skyrme-Hartree-Fock method in 3-dimensional coordinate space. Defining a reaction radius, $a_R=\sqrt{\sigma_R/\pi}$, to characterize the nuclear size and target (proton or $^{12}$C) dependence, we find an empirical formula for expressing $a_R$ with the point matter radius and the skin thickness, and assess two practical ways of determining the skin thickness from proton-nucleus $\sigma_R$ values measured at different energies or from $\sigma_R$ values measured for different targets.Comment: 6 pages, 5 figures, to appear in Phys. Rev.

Skin grafts : local quest for viable alternatives to autologous grafts using silk and acellular dermal matrices

The gold standard with regards to skin transplantation is the use of the patient’s own skin obtained from a healthy donor site. Such grafts can be either full thickness skin or more commonly nowadays, split thickness skin. Various materials, having either natural and or synthetic origins, have been used in the engineering of skin substitutes to-date and these grafts are then confronted against autologous skin grafts. If proven to be successful, such matrices could be utilised in clinical applications such as in the treatment of burn wounds and in cases of skin ulcers amongst others. In this study the primary cells used, keratinocytes and fibroblast, were obtained from donor skin and cultured. Scaffolds of xenogenic (raw silk) as well as of allogenic (acellular dermal matrices) origins were obtained via low-cost methods and seeded using the fibroblasts and keratinocytes so as to determine which gave the closest mimic to skin grafts. Out of the matrices assessed, the raw silk matrix allowed the best colonisation with skin cells in our hands. The ADM matrice also showed some cell colonisation, but will need further experimentation.peer-reviewe

A new method to determine the skin thickness of asymmetric UF-membranes using colloidal gold particles

In this paper a new method is presented for the determination of the skin thickness of asymmetric ultrafiltration membranes. The method is based on the use of well-defined, uniformly sized colloidal gold particles, permeated from the sublayer side of the membrane, combined with electron microscopic analysis of the membrane afterward. Using this method poly(2,6-dimethyl-1,4-phenylene oxide) (PPO) and polysulfone (PSf) membranes were investigated. PPO membranes appeared to have a well-defined skin layer with a thickness of about 0.2 μm and a pore size distinctly different from that in the macroporous layer underneath. In the case of PSf such a distinct skin layer cannot be defined. The size of the pores in these membranes gradually increases from skin to sublayer