Recent advances in our understanding of the structure and function of more unusual cation channels.

As their name implies, cation channels allow the regulated flow of cations such as sodium, potassium, calcium, and magnesium across cellular and intracellular membranes. Cation channels have long been known for their fundamental roles in controlling membrane potential and excitability in neurons and muscle. In this review, we provide an update on the recent advances in our understanding of the structure-function relationship and the physiological and pathophysiological role of cation channels. The most exciting developments in the last two years, in our opinion, have been the insights that cryoelectron microscopy has provided into the inner life and the gating of not only voltage-gated channels but also mechanosensitive and calcium- or sodium-activated channels. The mechanosensitive Piezo channels especially have delighted the field not only with a fascinating new type of structure but with important roles in blood pressure regulation and lung function

TRPV4, TRPC1, and TRPP2 assemble to form a flow-sensitive heteromeric channel

Transient receptor potential (TRP) channels, a superfamily of ion channels, can be divided into 7 subfamilies, including TRPV, TRPC, TRPP, and 4 others. Functional TRP channels are tetrameric complexes consisting of 4 pore-forming subunits. The purpose of this study was to explore the heteromerization of TRP subunits crossing different TRP subfamilies. Two-step coimmunoprecipitation (co-IP) and fluorescence resonance energy transfer (FRET) were used to determine the interaction of the different TRP subunits. Patch-clamp and cytosolic Ca2+ measurements were used to determine the functional role of the ion channels in flow conditions. The analysis demonstrated the formation of a heteromeric TRPV4-C1-P2 complex in primary cultured rat mesenteric artery endothelial cells (MAECs) and HEK293 cells that were cotransfected with TRPV4, TRPC1, and TRPP2. In functional experiments, pore-dead mutants for each of these 3 TRP isoforms nearly abolished the flow-induced cation currents and Ca2+ increase, suggesting that all 3 TRPs contribute to the ion permeation pore of the channels. We identified the first heteromeric TRP channels composed of subunits from 3 different TRP subfamilies. Functionally, this heteromeric TRPV4- C1-P2 channel mediates the flow-induced Ca2+ increase in native vascular endothelial cells.-Du, J., Ma, X., Shen, B., Huang, Y., Birnbaumer, L., Yao, X. TRPV4, TRPC1, and TRPP2 assemble to form a flowsensitive heteromeric channel.Fil: Du, Juan. Chinese University Of Hong Kong; Hong Kong. Anhui Medical University; ChinaFil: Ma, Xin. Chinese University Of Hong Kong; Hong KongFil: Shen, Bing. Chinese University Of Hong Kong; Hong Kong. Anhui Medical University; ChinaFil: Huang, Yu. Chinese University Of Hong Kong; Hong KongFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. National Institutes of Health; Estados UnidosFil: Yao, Xiaoqiang. Chinese University Of Hong Kong; Hong Kon

Investigation and Assessment of basic Flow Field Designs for the cathodic Gas Supply in low Temperature Fuel Cells

One of the major challenges in the development of polymer electrolyte fuel cells (PEFCs) is to exploit the whole potential that inheres a given membrane electrode assembly. For this purpose, the fuel cell performance can be maximised by optimising the flow field design with regard to the intended operating point. As a prerequisite for such an optimisation, the electrochemical performance of PEFCs with various cathodic flow fields (Parallel flow, serpentine, spiral and interdigitated) has been investigated. In addition, the influence of the rib width was studied by utilising each structure with different rib widths, whereby the width and the height of the channels remain constant. The characterisation of the gas distributor structures was carried out by measuring overall polarization curves and local current density distributions by means of a segmented laboratory cell with an active area of 25 cm2. The optimum gas distributor structure strongly depends on the particular operating point. Consequently, each basic structure has to be adapted for the intended operating conditions. For example, a serpentine flow field generates the highest current densities for high air fluxes whereas an interdigitated structure is preferred for small flow rates. In the same way it can be shown that a spiral structure produces the most uniform current density distributions, whereas the current density distribution of a flow field with parallel channels is extremely inhomogeneous and becomes unsteady for higher water generation rates. The variation of the rib width has shown that all areas with a high catalytic activity are situated directly over the gas channels. In contrast, the covered areas represent only a small fraction of the global performance, whereby the general tendency is independent of the basic structure of the flow field

Active transport in a channel: stabilisation by flow or thermodynamics

Recent experiments on active materials, such as dense bacterial suspensions and microtubule-kinesin motor mixtures, show a promising potential for achieving self-sustained flows. However, to develop active microfluidics it is necessary to understand the behaviour of active systems confined to channels. Therefore here we use continuum simulations to investigate the behaviour of active fluids in a two-dimensional channel. Motivated by the fact that most experimental systems show no ordering in the absence of activity, we concentrate on temperatures where there is no nematic order in the passive system, so that any nematic order is induced by the active flow. We systematically analyze the results, identify several different stable flow states, provide a phase diagram and show that the key parameters controlling the flow are the ratio of channel width to the length scale of active flow vortices, and whether the system is flow aligning or flow tumbling

Expanded microchannel heat exchanger: design, fabrication and preliminary experimental test

This paper first reviews non-traditional heat exchanger geometry, laser welding, practical issues with microchannel heat exchangers, and high effectiveness heat exchangers. Existing microchannel heat exchangers have low material costs, but high manufacturing costs. This paper presents a new expanded microchannel heat exchanger design and accompanying continuous manufacturing technique for potential low-cost production. Polymer heat exchangers have the potential for high effectiveness. The paper discusses one possible joining method - a new type of laser welding named "forward conduction welding," used to fabricate the prototype. The expanded heat exchanger has the potential to have counter-flow, cross-flow, or parallel-flow configurations, be used for all types of fluids, and be made of polymers, metals, or polymer-ceramic precursors. The cost and ineffectiveness reduction may be an order of magnitude or more, saving a large fraction of primary energy. The measured effectiveness of the prototype with 28 micron thick black low density polyethylene walls and counterflow, water-to-water heat transfer in 2 mm channels was 72%, but multiple low-cost stages could realize the potential of higher effectiveness

A superelement-based method for computing unsteady three-dimensional potential flows in hydraulic turbomachines

A numerical method is presented for the computation of unsteady, three-dimensional potential flows in hydraulic pumps and turbines. The superelement method has been extended in order to eliminate slave degrees of freedom not only from the governing Laplace equation, but also from the Kutta conditions. The resulting superelement formulation is invariant under rotation. Therefore the geometrical symmetry of the flow channels in the rotor can be exploited. This makes the method especially suitable to performing fully coupled computations of the unsteady flow phenomena in both rotor and stator, the so-called rotor-stator interaction. \ud The developed numerical method is used to simulate the unsteady flow in an industrial mixed-flow pump. Two types of simulation are considered: one in which unsteady wakes behind the trailing edges of the rotor blades are taken into account and one in which these are neglected. Results are given that show the importance of unsteady flow phenomena. However, the computed head-capacity curve is hardly influenced by whether or not unsteady wakes are taken into account