Phase Shifts of the Circadian Locomotor Rhythm Induced by Pigment-Dispersing Factor in the Cricket Gryllus bimaculatus

Pigment-dispersing factors (PDFs) are octadeca-peptides widely distributed in insect optic lobes and brain. In this study, we have purified PDF and determined its amino acid sequence in the cricket Gryllus bimaculatus. Its primary structure was NSEIINSLLGLPKVLNDA-NH2, homologous to other PDH family members so far reported. When injected into the optic lobe of experimentally blinded adult male crickets, Gryllus-PDF induced phase shifts in their activity rhythms in a phase dependent and dose dependent manner. The resulted phase response curve (PRC) showed delays during the late subjective night to early subjective day and advances during the mid subjective day to mid subjective night. The PRC was different in shape from those for light, serotonin and temperature. These results suggest that PDF plays a role in phase regulation of the circadian clock through a separate pathway from those of other known phase regulating agents

Multistable Phase Regulation for Robust Steady and Transitional Legged Gaits

We develop robust methods that allow specification, control, and transition of a multi-legged robot’s stepping pattern—its gait—during active locomotion over natural terrain. Resulting gaits emerge through the introduction of controllers that impose appropriately-placed repellors within the space of gaits, the torus of relative leg phases, thereby mitigating against dangerous patterns of leg timing. Moreover, these repellors are organized with respect to a natural cellular decomposition of gait space and result in limit cycles with associated basins that are well characterized by these cells, thus conferring a symbolic character upon the overall behavioral repertoire. These ideas are particularly applicable to four- and six-legged robots, for which a large variety of interesting and useful (and, in many cases, familiar) gaits exist, and whose tradeoffs between speed and reliability motivate the desire for transitioning between them during active locomotion. We provide an empirical instance of this gait regulation scheme by application to a climbing hexapod, whose “physical layer” sensor-feedback control requires adequate grasp of a climbing surface but whose closed loop control perturbs the robot from its desired gait. We document how the regulation scheme secures the desired gait and permits operator selection of different gaits as required during active climbing on challenging surfaces

Силовая электроника в смарт сетях

Discusses features of the criteria for evaluating the quality of electricity, and also integrated indicators on the basic and higher harmonics. Shown that power converters work with pulse-phase regulation of alternating voltage induces a change of reactive power component of the power network. Review the functions of power electronics devices in Smart Grids

Two Regulatory Aspects of INO1 Transcription in Yeast

This study focuses on understanding the mechanisms of expression control of a phospholipid biosynthetic gene, INO1. This study also includes investigation into transcriptional regulation of SNA3, a gene in tandem upstream of INO1. INO1 expression is a prevailing model for transcription studies. INO1 is repressed under growth conditions with inositol and derepressed by two transcription activators, Ino2 and Ino4, when inositol is absent. Coordination of the centromeric binding factor, Cbf1, with Ino2 and Ino4 is required for efficient derepression of INO1. Transcription of the INO1 adjacent SNA3 gene is also influenced by inositol. INO1 and SNA3 are co-regulated by Cbf1, Ino2 and Ino4. However, the mechanism of this co-regulation is not fully understood. A separate aspect of INO1 expression is its growth phase regulation. Under inositol depleted conditions, the expression of INO1 increases during log phase and decreases during stationary phase. Most genes in yeast are believed to be expressed at a constant level through all growth phases. It is unclear how INO1 growth phase regulation takes place. The first part of my work focused on exploring the mechanism through which Cbf1, Ino2 and Ino4 control the inositol-mediated regulation of INO1 and SNA3. This included determining the necessity of the Cbf1 binding site for Ino2 and Ino4 binding, as well as for the inositol mediated regulation of INO1 and SNA3. The second part of my work focused on understanding the growth phase regulation of INO1. This includes examining the expression of INO1 in individual cells in a growing population

Observing parity-time symmetry in diffusive systems

Phase modulation has scarcely been mentioned in diffusive systems since the diffusion process does not carry momentum like waves. Recently, the non-Hermitian physics provides a new perspective for understanding diffusion and shows prospects in the phase regulation of heat flow, for example, the discovery of anti-parity-time (APT) symmetry in diffusive systems. The precise control of thermal phase however remains elusive hitherto and can hardly be realized in APT-symmetric thermal systems due to the existence of phase oscillation. Here we construct the counterpart of APT-symmetric diffusive systems, i.e., PT-symmetric diffusive systems, which can achieve complete suppression of thermal phase oscillation. We find the real coupling of diffusive fields can be readily established through a strong convective background, where the decay-rate detuning is enabled by thermal metamaterial design. Moreover, we observe phase transition of PT symmetry breaking in diffusive systems with the symmetry-determined amplitude distribution and phase regulation of coupled temperature fields. Our work uncovers the existence of PT-symmetry in dissipative energy exchanges and provides a unique approach for harnessing the mass transfer of particles, the wave propagation in strongly scattering systems as well as thermal conduction

Single Walled Carbon Nanotubes Exhibit Dual-Phase Regulation to Exposed Arabidopsis Mesophyll Cells

Herein we are the first to report that single-walled carbon nanotubes (SWCNTs) exhibit dual-phase regulation to Arabidopsis mesophyll cells exposed to different concentration of SWCNTs. The mesophyll protoplasts were prepared by enzyme digestion, and incubated with 15, 25, 50, 100 μg/ml SWCNTs for 48 h, and then were observed by optical microscopy and transmission electron microscopy, the reactive oxygen species (ROS) generation was measured. Partial protoplasts were stained with propidium iodide and 4'-6- diamidino-2-phenylindole, partial protoplasts were incubated with fluorescein isothiocyanate-labeled SWCNTs, and observed by fluorescence microscopy. Results showed that SWCNTs could traverse both the plant cell wall and cell membrane, with less than or equal to 50 μg/ml in the culture medium, SWCNTs stimulated plant cells to grow out trichome clusters on their surface, with more than 50 μg/ml SWCNTs in the culture medium, SWCNTs exhibited obvious toxic effects to the protoplasts such as increasing generation of ROS, inducing changes of protoplast morphology, changing green leaves into yellow, and inducing protoplast cells' necrosis and apoptosis. In conclusion, single walled carbon nanotubes can get through Arabidopsis mesophyll cell wall and membrane, and exhibit dose-dependent dual-phase regulation to Arabidopsis mesophyll protoplasts such as low dose stimulating cell growth, and high dose inducing cells' ROS generation, necrosis or apoptosis

Dual phase regulation of experimental allergic encephalomyelitis by platelet-activating factor

Experimental allergic encephalomyelitis (EAE) serves as a model for multiple sclerosis and is considered to be a CD4+ Th1 cell–mediated autoimmune disease. To investigate the role of platelet-activating factor (PAF) in this disease, PAF receptor (PAFR) KO (PAFR-KO) and wild-type (WT) mice, on a C57BL/6 genetic background, were immunized with myelin oligodendrocyte glycoprotein 35–55. The levels of PAF production and PAFR mRNA expression in the spinal cord (SC) correlated with the EAE symptoms. PAFR-KO mice showed lower incidence and less severe symptoms in the chronic phase of EAE than WT mice. However, no difference was observed in T cell proliferation, Th1-cytokine production, or titer of IgG2a between both genotypes. Before onset, as revealed by microarray analysis, mRNAs of inflammatory mediators and their receptors—including IL-6 and CC chemokine receptor 2—were down-regulated in the SC of PAFR-KO mice compared with WT mice. Moreover, in the chronic phase, the severity of inflammation and demyelination in the SC was substantially reduced in PAFR-KO mice. PAFR-KO macrophages reduced phagocytic activity and subsequent production of TNF-α. These results suggest that PAF plays a dual role in EAE pathology in the induction and chronic phases through the T cell–independent pathways