Comprehensive Nontargeted Analysis of Drinking Water Supplies to Identify Chemicals Associated with Estrogen Receptor Agonism or Present in Regions of Elevated Breast Cancer Occurrence

To explore the hypothesis that differential exposures to estrogen active chemicals may contribute to regional disparities in cancer incidence, a comprehensive targeted and nontargeted analysis was conducted over two seasons (2020) for drinking water samples from 120 households served by 8 public water systems (4 with historically elevated breast cancer incidence) and from 15 brands of retail water. All samples were analyzed using gas and liquid chromatography with high-resolution mass spectrometry and a bioassay for estrogen receptor agonism. Target compounds included disinfection byproducts, per- and polyfluoroalkyl substances (PFAS), trace elements, and compounds selected for their possible relation to breast cancer. Over 7500 GC and LC nontargeted molecular features passed all quality control filters in each sampling season and were prioritized for identification if they were related to measured estrogen receptor agonism or were present at higher levels in areas with high breast cancer incidence (n = 1036). Benzothiazole-2-sulfonic acid, acetyl tributyl citrate, and diphenyl sulfone were among the prioritized and confirmed nontarget compounds. Nine polycyclic aromatic hydrocarbons and two ketone derivatives displayed significant negative correlations with estrogen receptor agonism. Many prioritized compounds remained unidentified, as 84.4% of the LC features and 77.5% of the GC features could not be annotated with high confidence

Occurrence and levels of selected compounds in European compost and digestate samples

This report describes work conducted by the European Commission’s Joint Research Centre (JRC) in the context of an Administrative Arrangement between DG Environment and the JRC. This work aimed at the generation, within a limited timeframe, of a large amount of analytical data, with high scientific and statistical value, for a number of compost and digestate types (afterwards referred to as COMDIG samples), to help provide a general overview and estimation of that possible variability within and between different COMDIG materials. The report includes the results of a targeted and independent screening of typical European situations of COMDIG materials with regard to the occurrence and levels of compounds of concern, many of which have never been assessed at a pan-European level. In total, 139 samples, mostly taken as grab samples and originating from 15 countries, were assessed for 22 minor and trace elements and 92 organic compounds including ingredients of personal care products and pharmaceuticals. The underlying analytical methods are carefully documented with regard to their performance characteristics. Where available, the so-called “horizontal” standards were followed. The results obtained are assessed statistically. Although the analysed single samples are insufficient to make any statement on the performance of the treatment processes leading to COMDIG samples, this collective of data provide a glimpse of the pan-European situation as regards the studied compounds.JRC.H.1-Water Resource

Effect of tunnel wash water treatment processes on trace elements, organic micropollutants, and biological effects

Tunnel wash water (TWW) contains high levels of trace elements and organic micropollutants, especially in the dissolved fraction. Discharge poses significant environmental risks. This field study aimed at improving conventional sedimentation treatment by addition of novel secondary treatments: bag filtration, ceramic microfiltration, or granular activated carbon (GAC) filtration. Removal of nine trace elements, 16 polycyclic aromatic hydrocarbons (PAHs), 38 per- and polyfluoroalkyl substances (PFASs), seven benzothiazoles (BTHs), seven benzotriazoles (BTRs), five bisphenols (BPs), and five benzophenones was investigated. Primary sedimentation significantly reduced particles and associated contaminants, achieving over 73 % average removal for trace elements, 65 % for PAHs, and 71 % for PFASs. Subsequent GAC removed over 70 % of dissolved Cr, Cu, Pb, and Zn and over 92 % of dissolved PFASs, BTHs, BTRs, and BPs, including several persistent, mobile and toxic compounds. Following GAC filtration, Cr, Ni, Pb, anthracene, fluoranthene, perfluorooctanesulfonic acid, and bisphenol-A were below environmental quality standards (EQS). GAC consistently reduced responses in in vitro bioassays with endpoints activation of the aryl hydrocarbon receptor, oxidative stress response, and neurotoxicity below effect-based trigger values for surface water. GAC filtration is thus recommended for future TWW treatment. Assessing water quality remains a challenging task due to lack of EQSs for many chemicals.Effect of tunnel wash water treatment processes on trace elements, organic micropollutants, and biological effectspublishedVersio

Pioneering an effect-based early warning system for hazardous chemicals in the environment

Existing regulatory frameworks often prove inadequate in promptly identifying contaminants of emerging concern (CECs) and determining their impacts on biological systems at an early stage. The establishment of Early Warning Systems (EWSs) for CECs is becoming increasingly relevant for policy-making, aiming to proactively detect chemical hazards and implement effective mitigation measures. Effect-based methodologies, including bioassays and effect-directed analysis (EDA), offer valuable input to EWSs by pinpointing the relevant toxicity drivers and prioritizing the associated risks. This review evaluates the analytical techniques currently available to assess biological effects, and provides a structured plan for their systematic integration into an EWS for hazardous chemicals in the environment. Key scientific advancements in effect-based approaches and EDA are discussed, underscoring their potential for early detection and management of chemical hazards. Additionally, critical challenges such as data integration and regulatory alignment are addressed, emphasizing the need for continuous improvement of the EWS and the incorporation of analytical advancements to safeguard environmental and public health from emerging chemical threats.Pioneering an effect-based early warning system for hazardous chemicals in the environmentpublishedVersio

Pioneering an effect-based early warning system for hazardous chemicals in the environment

Existing regulatory frameworks often prove inadequate in identifying contaminants of emerging concern (CECs) and determining their impacts on biological systems at an early stage. The establishment of Early Warning Systems (EWSs) for CECs is becoming increasingly relevant for policy-making, aiming to proactively detect chemical hazards and implement effective mitigation measures. Effect-based methodologies, including bioassays and effect-directed analysis (EDA), offer valuable input to EWSs with a view to pinpointing the relevant toxicity drivers and prioritizing the associated risks. This review evaluates the analytical techniques currently available to assess biological effects, and provides a structured plan for their systematic integration into an EWS for hazardous chemicals in the environment. Key scientific advancements in effect-based approaches and EDA are discussed, underscoring their potential for early detection and management of chemical hazards. Additionally, critical challenges such as data integration and regulatory alignment are addressed, emphasizing the need for continuous improvement of the EWS and the incorporation of analytical advancements to safeguard environmental and public health from emerging chemical threats

Effect based methods for monitoring and assessment of aquatic ecosystems: applications and future perspectives

The thesis is about the application of Effect Based Methods (EBM) for the monitoring and assessment of aquatic ecosystems. This research approach has been developed in the last years and has the aim to contribute to identify potential chemical risks for aquatic ecosystems and indirectly for human health. The identification of these risks is a key aspect for the application of prevention and policy measures needed to protect environment and human health. The Effect Based Methods (Bioassays in vivo and in vitro, biomarkers) have been recommended in the context of the Common Implementation Strategy of the European Water Framework Directive that is an ambitious European legislative act with the key aim to achieve a good status for all water bodies in Europe. EBM are tools used for the monitoring of waterbodies and other environmental compartments with the aim to detect effect caused by pollutants or group of pollutants, these effects can be detected at molecular, cellular, individual or populational level. During the 3 years of the project an extensive evaluation of these methods in Europe has been carried out and the potentiality to be implemented in the European and national legislation has been highlighted. Furthermore the possibility to prevent indirect effects on human health has also been considered. A specific case study has been dedicated to the FET test (Fish embryo toxicity test) that is a bioassay widely applied in this field. Bioassays have been applied experimentally in the Tiber river basin, mainly in the urban part, to identify the presence of potential effects caused by mixtures of chemical pollutants and/or emerging substances. These methods have been applied also to investigate the causes of a specific event that happened in 2020 and that has caused a massive fish kills in Tiber river. In conclusion, based on the research carried out, these methods could be considered mature enough to be included in the legislative framework for the protection of water resources