8,904 research outputs found

    Homeostasis in Immunity-Related Pupal Tissues of the Malaria Mosquito Anopheles gambiae and its regulation by the NF-kappaB-like Factor Rel2

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    Die Haut ist eine oft ĂŒbersehene Komponente des angeborenen Immunsystems der MĂŒcken. Die Haut der MĂŒcke bildet eine physische Barriere, die die mikrobielle Homöostase aufrechterhĂ€lt, das Eindringen von Toxinen wie Insektiziden verhindert und das Austrocknen verhindert. Die am meisten untersuchten Akteure des Immunsystems von StechmĂŒcken sind das Fettgewebe und die Blutzellen, aber die Hauttalg-Fabriken, die Oenozyten, werden in Studien nur selten berĂŒcksichtigt. MĂŒckenpuppen haben aktiv funktionierende immunitĂ€tsbezogene Organe, einschließlich derjenigen, die Hautbarrieren produzieren. Ihre biologische Rolle in diesem Entwicklungsstadium ist kaum bekannt, aber der Übergang von der Puppen- zur Erwachsenenhaut und die AuffĂ€lligkeit der talgproduzierenden Zellen machen dieses Stadium zu einem vielversprechenden Entwicklungsstadium fĂŒr die Untersuchung der Hautbildung. Mit Hilfe der Transkriptomanalyse beschreiben wir die Rolle der Blutzellen bei der Entwicklung des chitinösen Teils der Insektenhaut, die Beteiligung des Fettkörpers an der ImmunitĂ€t und bestĂ€tigen die Rolle der talgproduzierenden Zellen im Lipidstoffwechsel. DarĂŒber hinaus beschreiben wir talgsezernierende Zellen als einen bedeutenden Wirkungsort des NF-kappaB-Ă€hnlichen IMD-Rel2-Pathway, in dem der Transkriptionsfaktor Rel2 die Retinoid-Homöostase reguliert. Schließlich bestĂ€tigen wir eine 100 Jahre alte Beobachtung, wonach sebumsezernierende Zellen der StechmĂŒcke ihren Zellinhalt in einem Netzwerk von Vesikeln absondern. Wir beschreiben extrazellulĂ€res Chromatin als Fracht in diesem Vesikelnetzwerk und sein antimikrobielles Potenzial.The skin is an often overlooked component of the mosquito's innate immune system. The mosquito skin provides a physical barrier that maintains microbial homeostasis, prevents the entry of toxins like insecticides, and avoids desiccation. The most studied players in the immune system of mosquitoes are the adipose tissue and blood cells, but studies rarely consider the skin sebum factories, oenocytes. Mosquito pupae have actively functional immunity-related organs, including those producing skin barriers. Their biological roles at this developmental stage are poorly understood, but the pupae-to-adult metamorphic skin transition and the conspicuity of sebum-secreting cells make it a promising developmental stage to study skin formation. We use transcriptomics to describe the role of blood cells in the development of the chitinous section of the insect skin, the involvement of the fat body in immunity, and confirm the lipid metabolism role of sebum-secreting cells. Furthermore, we describe sebum-secreting cells as a significant action site of the NF-kappaB-like IMD-Rel2 pathway where the transcription factor Rel2 regulates retinoid homeostasis. Finally, we confirm a 100-year-old observation of how mosquito sebum-secreting cells secrete their cellular contents in a network of vesicles. We describe extracellular chromatin as cargo inside this vesicle network and its antimicrobial potential

    Modeling the Growth of Six Listeria monocytogenes Strains in Smoked Salmon Pùté

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    In this study, the growth of six L. monocytogenes strains isolated from different fish products was quantified and modeled in smoked salmon pĂątĂ© at a temperature ranging from 2 to 20 °C. The experimental data obtained for each strain was fitted to the primary growth model of Baranyi and Roberts to estimate the following kinetic parameters: lag phase (λ), maximum specific growth rate (ÎŒmax), and maximum cell density (Nmax). Then, the effect of storage temperature on the obtained ÎŒmax values was modeled by the Ratkowsky secondary model. In general, the six L. monocytogenes strains showed rapid growth in salmon pĂątĂ© at all storage temperatures, with a relatively short lag phase λ, even at 2 °C. The growth behavior among the tested strains was similar at the same storage temperature, although significant differences were found for the parameters λ and ÎŒmax. Besides, the growth variations among the strains did not follow a regular pattern. The estimated secondary model parameter Tmin ranged from −4.25 to −3.19 °C. This study provides accurate predictive models for the growth of L. monocytogenes in fish pĂątĂ©s that can be used in shelf life and microbial risk assessment studies. In addition, the models generated in this work can be implemented in predictive modeling tools and repositories that can be reliably and easily used by the fish industry and end-users to establish measures aimed at controlling the growth of L. monocytogenes in fish-based pĂątĂ©s

    Sourcing high tissue quality brains from deceased wild primates with known socio‐ecology

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    The selection pressures that drove dramatic encephalisation processes through the mammal lineage remain elusive, as does knowledge of brain structure reorganisation through this process. In particular, considerable structural brain changes are present across the primate lineage, culminating in the complex human brain that allows for unique behaviours such as language and sophisticated tool use. To understand this evolution, a diverse sample set of humans' closest relatives with varying socio-ecologies is needed. However, current brain banks predominantly curate brains from primates that died in zoological gardens. We try to address this gap by establishing a field pipeline mitigating the challenges associated with brain extractions of wild primates in their natural habitat. The success of our approach is demonstrated by our ability to acquire a novel brain sample of deceased primates with highly variable socio-ecological exposure and a particular focus on wild chimpanzees. Methods in acquiring brain tissue from wild settings are comprehensively explained, highlighting the feasibility of conducting brain extraction procedures under strict biosafety measures by trained veterinarians in field sites. Brains are assessed at a fine-structural level via high-resolution MRI and state-of-the-art histology. Analyses confirm that excellent tissue quality of primate brains sourced in the field can be achieved with a comparable tissue quality of brains acquired from zoo-living primates. Our field methods are noninvasive, here defined as not harming living animals, and may be applied to other mammal systems than primates. In sum, the field protocol and methodological pipeline validated here pose a major advance for assessing the influence of socio-ecology on medium to large mammal brains, at both macro- and microstructural levels as well as aiding with the functional annotation of brain regions and neuronal pathways via specific behaviour assessments.Output Status: Forthcoming/Available Online Additional authors: Richard McElreath, Alfred Anwander, Philipp Gunz, Markus Morawski, Angela D. Friederici, Nikolaus Weiskopf, Fabian H. Leendertz, Roman M. Wittig EBC Cosortium: Karoline Albig, Bala Amarasekaran, Sam Angedakin, Alfred Anwander, Daniel Aschoff, Caroline Asiimwe, Laurent Bailanda, Jacinta C. Beehner, Raphael Belais, Thore J. Bergman, Birgit Blazey, Andreas Bernhard, Christian Bock, PĂ©nĂ©lope Carlier, Julian Chantrey, Catherine Crockford, Tobias Deschner, Ariane DĂŒx1, Luke Edwards, Cornelius Eichner, GĂ©raldine Escoubas2, Malak Ettaj, Karina Flores, Richard Francke, Angela D. Friederici, CĂ©dric Girard-Buttoz, Jorge Gomez Fortun, Zoro Bertin GoneBi, Tobias GrĂ€ĂŸle, Eva Gruber-Dujardin, Philipp Gunz, Jess Hartel, Daniel B. M. Haun, Michael Henshall, Catherine Hobaiter, NoĂ©mie Hofman, Jenny E. Jaffe, Carsten JĂ€ger, Anna Jauch, Stomy Kahemere, Evgeniya Kirilina, Robert Klopfleisch, Tobias Knauf-Witzens, Kathrin S. Kopp, Guy Landry Mamboundou Kouima, Bastian Lange, Kevin Langergraber, Arne Lawrenz, Fabian H. Leendertz, Ilona Lipp, Matys Liptovszky, Tobias Loubser Theron, Christelle Patricia Lumbu, Patrice Makouloutou Nzassi, Kerstin MĂ€tz-Rensing, Richard McElreath, Matthew McLennan, Zoltan Mezö, Sophie Moittie, Torsten MĂžller, Markus Morawski, David Morgan, Timothy Mugabe, Martin Muller, Matthias MĂŒller, Inoussa Njumboket, Karin Olofsson-Sannö, Alain Ondzie, Emily Otali, Michael Paquette, Simone Pika, Kerrin Pine, Andrea Pizarro, Kamilla PlĂ©h, Jessica Rendel, Sandra Reichler-Danielowski, Martha M. Robbins, Alejandra Romero Forero, Konstantin Ruske, Liran Samuni, Crickette Sanz, AndrĂ© SchĂŒle, Ingo Schwabe, Katarina Schwalm, Sheri Speede, Lara Southern, Jonas Steiner, Marc Stidworthy, Martin Surbeck, Claudia Szentiks, Tanguy Tanga, Reiner Ulrich, Steve Unwin, Erica van de Waal, Sue Walker, Nikolaus Weiskopf, Gudrun Wibbelt, Roman M. Wittig, Kim Wood, Klaus ZuberbĂŒhle

    CO or no CO? Narrowing the CO abundance constraint and recovering the H2O detection in the atmosphere of WASP-127 b using SPIRou

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    Precise measurements of chemical abundances in planetary atmospheres are necessary to constrain the formation histories of exoplanets. A recent study of WASP-127b, a close-in puffy sub-Saturn orbiting its solar-type host star in 4.2 d, using HST and Spitzer revealed a feature-rich transmission spectrum with strong excess absorption at 4.5 um. However, the limited spectral resolution and coverage of these instruments could not distinguish between CO and/or CO2 absorption causing this signal, with both low and high C/O ratio scenarios being possible. Here we present near-infrared (0.9--2.5 um) transit observations of WASP-127 b using the high-resolution SPIRou spectrograph, with the goal to disentangle CO from CO2 through the 2.3 um CO band. With SPIRou, we detect H2O at a t-test significance of 5.3 sigma and observe a tentative (3 sigma) signal consistent with OH absorption. From a joint SPIRou + HST + Spitzer retrieval analysis, we rule out a CO-rich scenario by placing an upper limit on the CO abundance of log10[CO]<-4.0, and estimate a log10[CO2] of -3.7^(+0.8)_(-0.6), which is the level needed to match the excess absorption seen at 4.5um. We also set abundance constraints on other major C-, O-, and N-bearing molecules, with our results favoring low C/O (0.10^(+0.10)_(-0.06)), disequilibrium chemistry scenarios. We further discuss the implications of our results in the context of planet formation. Additional observations at high and low-resolution will be needed to confirm these results and better our understanding of this unusual world.Comment: 23 pages, 13 figures, Submitted for publication in the Monthly Notice of the Royal Astronomical Societ

    Feature Papers in Compounds

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    This book represents a collection of contributions in the field of the synthesis and characterization of chemical compounds, natural products, chemical reactivity, and computational chemistry. Among its contents, the reader will find high-quality, peer-reviewed research and review articles that were published in the open access journal Compounds by members of the Editorial Board and the authors invited by the Editorial Office and Editor-in-Chief

    Exploring QCD matter in extreme conditions with Machine Learning

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    In recent years, machine learning has emerged as a powerful computational tool and novel problem-solving perspective for physics, offering new avenues for studying strongly interacting QCD matter properties under extreme conditions. This review article aims to provide an overview of the current state of this intersection of fields, focusing on the application of machine learning to theoretical studies in high energy nuclear physics. It covers diverse aspects, including heavy ion collisions, lattice field theory, and neutron stars, and discuss how machine learning can be used to explore and facilitate the physics goals of understanding QCD matter. The review also provides a commonality overview from a methodology perspective, from data-driven perspective to physics-driven perspective. We conclude by discussing the challenges and future prospects of machine learning applications in high energy nuclear physics, also underscoring the importance of incorporating physics priors into the purely data-driven learning toolbox. This review highlights the critical role of machine learning as a valuable computational paradigm for advancing physics exploration in high energy nuclear physics.Comment: 146 pages,53 figure

    Against Imperial Arbitrators: The Brilliance of Canada\u27s New Model Investment Treaty

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    Investment treaty arbitration has become politically “toxic” even in states that pioneered the development of investment treaties. There is consensus on the need for reform. But there is a dearth of historical research on what went wrong with investment treaties, when it happened, or how to find the way forward in light of the past. As a result, reform efforts have a stumbling quality. One can see this in multilateral fora, such as the United Nations Commission on International Trade Law (UNCITRAL), where over four years of study and negotiations have produced little consensus. One can also see it in the investment treaty practice of individual states, such as Canada, which has recently lurched across the spectrum from investment treaty arbitration to a permanent international investment court, to the abandonment of investor-state dispute settlement (ISDS), and back to investment treaty arbitration. This article fills the gap in understanding by explaining what went wrong with investment treaty arbitration and when it happened. It demonstrates that the customary international law on state responsibility for injuries to aliens evolved during the 19th century to protect foreign investors against exceptional failures of the nightwatchman and rule-of-law states. As the consensus regarding customary international law standards of treatment unraveled during the 20th century due to the spread of communism, decolonization, and economic nationalism, capital-exporting states turned in bilateral investment treaties (BITs) to uphold traditional principles regarding the protection of foreign investment. Starting in the late 1990s, however, an unexpected surge of claims brought under NAFTA’s investment chapter fortuitously opened the door to the central problem of modern investment treaty practice: the rise of “imperial arbitrators” who do not merely police exceptional failures of the nightwatchman and rule-of-law states, but who choose to second-guess the normal operations of modern regulatory states without any meaningful checks or balances. Although the NAFTA Parties nipped that development in the bud, the rise of imperial arbitrators leapt to the broader universe of investment treaty arbitration, where it flourished until claims against developed states for measures such as the phaseout of nuclear power brought investment treaty arbitration to a crisis point. Seeking a way forward in light of the past, the article examines Canada’s recent experimentation with investment treaty reforms, including the development of a permanent international investment court in relations with the EU, the complete elimination of ISDS in relations with the United States, and a return to traditional investment treaty arbitration in a new model investment treaty coupled with substantive reforms that virtually eliminate opportunities to second-guess the normal operations of modern regulatory states. The article describes the last option as the most brilliant because it is the only one that substantively eliminates toeholds for imperial arbitrators while preserving arbitration as a safeguard against the exceptional failures of the nightwatchman and rule-of-law states. Seeking a way forward in light of the past, the article examines Canada’s recent experimentation with investment treaty reforms, including the development of a permanent international investment court in relations with the EU, the complete elimination of ISDS in relations with the United States, and a return to traditional investment treaty arbitration in a new model investment treaty coupled with substantive reforms that virtually eliminate opportunities to second-guess the normal operations of modern regulatory states. The article describes the last option as the most brilliant because it is the only one that substantively eliminates toeholds for imperial arbitrators while preserving arbitration as a safeguard against the exceptional failures of the nightwatchman and rule-of-law states
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