Hyperspectral colon tissue cell classification

A novel algorithm to discriminate between normal and malignant tissue cells of the human colon is presented. The microscopic level images of human colon tissue cells were acquired using hyperspectral imaging technology at contiguous wavelength intervals of visible light. While hyperspectral imagery data provides a wealth of information, its large size normally means high computational processing complexity. Several methods exist to avoid the so-called curse of dimensionality and hence reduce the computational complexity. In this study, we experimented with Principal Component Analysis (PCA) and two modifications of Independent Component Analysis (ICA). In the first stage of the algorithm, the extracted components are used to separate four constituent parts of the colon tissue: nuclei, cytoplasm, lamina propria, and lumen. The segmentation is performed in an unsupervised fashion using the nearest centroid clustering algorithm. The segmented image is further used, in the second stage of the classification algorithm, to exploit the spatial relationship between the labeled constituent parts. Experimental results using supervised Support Vector Machines (SVM) classification based on multiscale morphological features reveal the discrimination between normal and malignant tissue cells with a reasonable degree of accuracy

Spectral mixture analysis of EELS spectrum-images

Recent advances in detectors and computer science have enabled the acquisition and the processing of multidimensional datasets, in particular in the field of spectral imaging. Benefiting from these new developments, earth scientists try to recover the reflectance spectra of macroscopic materials (e.g., water, grass, mineral types...) present in an observed scene and to estimate their respective proportions in each mixed pixel of the acquired image. This task is usually referred to as spectral mixture analysis or spectral unmixing (SU). SU aims at decomposing the measured pixel spectrum into a collection of constituent spectra, called endmembers, and a set of corresponding fractions (abundances) that indicate the proportion of each endmember present in the pixel. Similarly, when processing spectrum-images, microscopists usually try to map elemental, physical and chemical state information of a given material. This paper reports how a SU algorithm dedicated to remote sensing hyperspectral images can be successfully applied to analyze spectrum-image resulting from electron energy-loss spectroscopy (EELS). SU generally overcomes standard limitations inherent to other multivariate statistical analysis methods, such as principal component analysis (PCA) or independent component analysis (ICA), that have been previously used to analyze EELS maps. Indeed, ICA and PCA may perform poorly for linear spectral mixture analysis due to the strong dependence between the abundances of the different materials. One example is presented here to demonstrate the potential of this technique for EELS analysis.Comment: Manuscript accepted for publication in Ultramicroscop

Wavelet based segmentation of hyperspectral colon tissue imagery

Segmentation is an early stage for the automated classification of tissue cells between normal and malignant types. We present an algorithm for unsupervised segmentation of images of hyperspectral human colon tissue cells into their constituent parts by exploiting the spatial relationship between these constituent parts. This is done by employing a modification of the conventional wavelet based texture analysis, on the projection of hyperspectral image data in the first principal component direction. Results show that our algorithm is comparable to other more computationally intensive methods which exploit the spectral characteristics of the hyperspectral imagery data

Bayesian estimation of linear mixtures using the normal compositional model. Application to hyperspectral imagery

This paper studies a new Bayesian unmixing algorithm for hyperspectral images. Each pixel of the image is modeled as a linear combination of so-called endmembers. These endmembers are supposed to be random in order to model uncertainties regarding their knowledge. More precisely, we model endmembers as Gaussian vectors whose means have been determined using an endmember extraction algorithm such as the famous N-finder (N-FINDR) or Vertex Component Analysis (VCA) algorithms. This paper proposes to estimate the mixture coefficients (referred to as abundances) using a Bayesian algorithm. Suitable priors are assigned to the abundances in order to satisfy positivity and additivity constraints whereas conjugate priors are chosen for the remaining parameters. A hybrid Gibbs sampler is then constructed to generate abundance and variance samples distributed according to the joint posterior of the abundances and noise variances. The performance of the proposed methodology is evaluated by comparison with other unmixing algorithms on synthetic and real images