Airborne and Terrestrial Laser Scanning Data for the Assessment of Standing and Lying Deadwood: Current Situation and New Perspectives

LiDAR technology is finding uses in the forest sector, not only for surveys in producing forests but also as a tool to gain a deeper understanding of the importance of the three-dimensional component of forest environments. Developments of platforms and sensors in the last decades have highlighted the capacity of this technology to catch relevant details, even at finer scales. This drives its usage towards more ecological topics and applications for forest management. In recent years, nature protection policies have been focusing on deadwood as a key element for the health of forest ecosystems and wide-scale assessments are necessary for the planning process on a landscape scale. Initial studies showed promising results in the identification of bigger deadwood components (e.g., snags, logs, stumps), employing data not specifically collected for the purpose. Nevertheless, many efforts should still be made to transfer the available methodologies to an operational level. Newly available platforms (e.g., Mobile Laser Scanner) and sensors (e.g., Multispectral Laser Scanner) might provide new opportunities for this field of study in the near future

Moving Target Defense for Web Applications

abstract: Web applications continue to remain as the most popular method of interaction for businesses over the Internet. With it's simplicity of use and management, they often function as the "front door" for many companies. As such, they are a critical component of the security ecosystem as vulnerabilities present in these systems could potentially allow malicious users access to sensitive business and personal data. The inherent nature of web applications enables anyone to access them anytime and anywhere, this includes any malicious actors looking to exploit vulnerabilities present in the web application. In addition, the static configurations of these web applications enables attackers the opportunity to perform reconnaissance at their leisure, increasing their success rate by allowing them time to discover information on the system. On the other hand, defenders are often at a disadvantage as they do not have the same temporal opportunity that attackers possess in order to perform counter-reconnaissance. Lastly, the unchanging nature of web applications results in undiscovered vulnerabilities to remain open for exploitation, requiring developers to adopt a reactive approach that is often delayed or to anticipate and prepare for all possible attacks which is often cost-prohibitive. Moving Target Defense (MTD) seeks to remove the attackers' advantage by reducing the information asymmetry between the attacker and defender. This research explores the concept of MTD and the various methods of applying MTD to secure Web Applications. In particular, MTD concepts are applied to web applications by implementing an automated application diversifier that aims to mitigate specific classes of web application vulnerabilities and exploits. Evaluation is done using two open source web applications to determine the effectiveness of the MTD implementation. Though developed for the chosen applications, the automation process can be customized to fit a variety of applications.Dissertation/ThesisMasters Thesis Computer Science 201

LiDAR based Biomass Estimation System for Forested Areas

In continental Portugal, forest fires are considered the biggest and most serious cause of forest deterioration and therefore the introduction of forest management mechanisms and biomass monitoring are imperative for a better future. However, conducting field studies on a large scale is a very expensive and time-consuming task. Alternatively, through remote sensing via a LiDAR, it becomes possible to map, with high accuracy, forest parameters such as tree height, diameter at breast height or tree canopy length in order to carry out other relevant estimates such as above ground biomass. In this sense, this dissertation aims to develop a system capable of, through algorithms and filters of point cloud processing, as statistical outlier removal, progressive morphological filters and region growing segmentation, extract in detail,a digital terrain model and correctly detect the number of trees in a given area, proceeding to the measurement of some interesting variables from the point of view of a forest inventory. Thus, testing data of different characteristics, our detection method obtained positive results, with all the average detection rates above 80 %.Em Portugal continental, os incêndios florestais são considerados a maior e mais grave causa de deterioramento da floresta e por isso a introdução de mecanismos de gestão florestal e monitorização da biomassa são imperativos para um futuro melhor. No entanto, realizar estudos de campo em grande escala é uma tarefa muito dispendiosa e demorosa. Em alternativa, através da deteção remota por vias de um LiDAR torna-se possível mapear, com elevado rigor, parâmetros florestais como altura das arvores, diâmetro do tronco ou comprimento da copa da arvore de modo a proceder a outras relevantes estimações como a biomassa. Neste sentido, esta dissertação teve como objetivo o desenvolvimento de um sistema capaz de, através de algoritmos e filtros de processamento de nuvens de pontos, como remoção de outliers estatístico, filtros morfologicos progressivos e segmentação por crescimento de regiões anexas , extrair com detalhe, um modelo digital do terreno e detetar corretamente o número de arvores numa determinada área, procedendo à medição de algumas variáveis interessantes do ponto de vista do inventário florestal. Assim, testando dados de diferentes características, o nosso método de deteção obteve resultados positivos, com todas as taxas deteção média superiores a 80 %

A tetraspecific VHH-based neutralizing antibody modifies disease outcome in three animal models of Clostridium difficile infection

Clostridium difficile infection (CDI), a leading cause of nosocomial infection, is a serious disease in North America, Europe, and Asia. CDI varies greatly from asymptomatic carriage to life-threatening diarrhea, toxic megacolon, and toxemia. The incidence of community-acquired infection has increased due to the emergence of hypervirulent antibiotic-resistant strains. These new strains contribute to the frequent occurrence of disease relapse, complicating treatment, increasing hospital stays, and increasing morbidity and mortality among patients. Therefore, it is critical to develop new therapeutic approaches that bypass the development of antimicrobial resistance and avoid disruption of gut microflora. Here, we describe the construction of a single heteromultimeric VHH-based neutralizing agent (VNA) that targets the two primary virulence factors of Clostridium difficile, toxins A (TcdA) and B (TcdB). Designated VNA2-Tcd, this agent has subnanomolar toxin neutralization potencies for both C. difficile toxins in cell assays. When given systemically by parenteral administration, VNA2-Tcd protected against CDI in gnotobiotic piglets and mice and to a lesser extent in hamsters. Protection from CDI was also observed in gnotobiotic piglets treated by gene therapy with an adenovirus that promoted the expression of VNA2-Tcd

Ninth Workshop and Tutorial on Practical Use of Coloured Petri Nets and the CPN Tools, Aarhus, Denmark, October 20-22, 2008

This booklet contains the proceedings of the Ninth Workshop on Practical Use of Coloured Petri Nets and the CPN Tools, October 20-22, 2008. The workshop is organised by the CPN group at the Department of Computer Science, University of Aarhus, Denmark. The papers are also available in electronic form via the web pages: http://www.daimi.au.dk/CPnets/workshop0

Evaluation of machine learning techniques for intrusion detection in software defined networking

Abstract. The widespread growth of the Internet paved the way for the need of a new network architecture which was filled by Software Defined Networking (SDN). SDN separated the control and data planes to overcome the challenges that came along with the rapid growth and complexity of the network architecture. However, centralizing the new architecture also introduced new security challenges and created the demand for stronger security measures. The focus is on the Intrusion Detection System (IDS) for a Distributed Denial of Service (DDoS) attack which is a serious threat to the network system. There are several ways of detecting an attack and with the rapid growth of machine learning (ML) and artificial intelligence, the study evaluates several ML algorithms for detecting DDoS attacks on the system. Several factors have an effect on the performance of ML based IDS in SDN. Feature selection, training dataset, and implementation of the classifying models are some of the important factors. The balance between usage of resources and the performance of the implemented model is important. The model implemented in the thesis uses a dataset created from the traffic flow within the system and models being used are Support Vector Machine (SVM), Naive-Bayes, Decision Tree and Logistic Regression. The accuracy of the models has been over 95% apart from Logistic Regression which has 90% accuracy. The ML based algorithm has been more accurate than the non-ML based algorithm. It learns from different features of the traffic flow to differentiate between normal traffic and attack traffic. Most of the previously implemented ML based IDS are based on public datasets. Using a dataset created from the flow of the experimental environment allows training of the model from a real-time dataset. However, the experiment only detects the traffic and does not take any action. However, these promising results can be used for further development of the model

Global gene expression profiling of healthy human brain and its application in studying neurological disorders

The human brain is the most complex structure known to mankind and one of the greatest challenges in modern biology is to understand how it is built and organized. The power of the brain arises from its variety of cells and structures, and ultimately where and when different genes are switched on and off throughout the brain tissue. In other words, brain function depends on the precise regulation of gene expression in its sub-anatomical structures. But, our understanding of the complexity and dynamics of the transcriptome of the human brain is still incomplete. To fill in the need, we designed a gene expression model that accurately defines the consistent blueprint of the brain transcriptome; thereby, identifying the core brain specific transcriptional processes conserved across individuals. Functionally characterizing this model would provide profound insights into the transcriptional landscape, biological pathways and the expression distribution of neurotransmitter systems. Here, in this dissertation we developed an expression model by capturing the similarly expressed gene patterns across congruently annotated brain structures in six individual brains by using data from the Allen Brain Atlas (ABA). We found that 84% of genes are expressed in at least one of the 190 brain structures. By employing hierarchical clustering we were able to show that distinct structures of a bigger brain region can cluster together while still retaining their expression identity. Further, weighted correlation network analysis identified 19 robust modules of coexpressing genes in the brain that demonstrated a wide range of functional associations. Since signatures of local phenomena can be masked by larger signatures, we performed local analysis on each distinct brain structure. Pathway and gene ontology enrichment analysis on these structures showed, striking enrichment for brain region specific processes. Besides, we also mapped the structural distribution of the gene expression profiles of genes associated with major neurotransmission systems in the human. We also postulated the utility of healthy brain tissue gene expression to predict potential genes involved in a neurological disorder, in the absence of data from diseased tissues. To this end, we developed a supervised classification model, which achieved an accuracy of 84% and an AUC (Area Under the Curve) of 0.81 from ROC plots, for predicting autism-implicated genes using the healthy expression model as the baseline. This study represents the first use of healthy brain gene expression to predict the scope of genes in autism implication and this generic methodology can be applied to predict genes involved in other neurological disorders