3,762 research outputs found
Multi-stage Multi-recursive-input Fully Convolutional Networks for Neuronal Boundary Detection
In the field of connectomics, neuroscientists seek to identify cortical
connectivity comprehensively. Neuronal boundary detection from the Electron
Microscopy (EM) images is often done to assist the automatic reconstruction of
neuronal circuit. But the segmentation of EM images is a challenging problem,
as it requires the detector to be able to detect both filament-like thin and
blob-like thick membrane, while suppressing the ambiguous intracellular
structure. In this paper, we propose multi-stage multi-recursive-input fully
convolutional networks to address this problem. The multiple recursive inputs
for one stage, i.e., the multiple side outputs with different receptive field
sizes learned from the lower stage, provide multi-scale contextual boundary
information for the consecutive learning. This design is
biologically-plausible, as it likes a human visual system to compare different
possible segmentation solutions to address the ambiguous boundary issue. Our
multi-stage networks are trained end-to-end. It achieves promising results on
two public available EM segmentation datasets, the mouse piriform cortex
dataset and the ISBI 2012 EM dataset.Comment: Accepted by ICCV201
U-Net: Convolutional Networks for Biomedical Image Segmentation
There is large consent that successful training of deep networks requires
many thousand annotated training samples. In this paper, we present a network
and training strategy that relies on the strong use of data augmentation to use
the available annotated samples more efficiently. The architecture consists of
a contracting path to capture context and a symmetric expanding path that
enables precise localization. We show that such a network can be trained
end-to-end from very few images and outperforms the prior best method (a
sliding-window convolutional network) on the ISBI challenge for segmentation of
neuronal structures in electron microscopic stacks. Using the same network
trained on transmitted light microscopy images (phase contrast and DIC) we won
the ISBI cell tracking challenge 2015 in these categories by a large margin.
Moreover, the network is fast. Segmentation of a 512x512 image takes less than
a second on a recent GPU. The full implementation (based on Caffe) and the
trained networks are available at
http://lmb.informatik.uni-freiburg.de/people/ronneber/u-net .Comment: conditionally accepted at MICCAI 201
Recursive Training of 2D-3D Convolutional Networks for Neuronal Boundary Detection
Efforts to automate the reconstruction of neural circuits from 3D electron
microscopic (EM) brain images are critical for the field of connectomics. An
important computation for reconstruction is the detection of neuronal
boundaries. Images acquired by serial section EM, a leading 3D EM technique,
are highly anisotropic, with inferior quality along the third dimension. For
such images, the 2D max-pooling convolutional network has set the standard for
performance at boundary detection. Here we achieve a substantial gain in
accuracy through three innovations. Following the trend towards deeper networks
for object recognition, we use a much deeper network than previously employed
for boundary detection. Second, we incorporate 3D as well as 2D filters, to
enable computations that use 3D context. Finally, we adopt a recursively
trained architecture in which a first network generates a preliminary boundary
map that is provided as input along with the original image to a second network
that generates a final boundary map. Backpropagation training is accelerated by
ZNN, a new implementation of 3D convolutional networks that uses multicore CPU
parallelism for speed. Our hybrid 2D-3D architecture could be more generally
applicable to other types of anisotropic 3D images, including video, and our
recursive framework for any image labeling problem
Modeling Brain Circuitry over a Wide Range of Scales
If we are ever to unravel the mysteries of brain function at its most
fundamental level, we will need a precise understanding of how its component
neurons connect to each other. Electron Microscopes (EM) can now provide the
nanometer resolution that is needed to image synapses, and therefore
connections, while Light Microscopes (LM) see at the micrometer resolution
required to model the 3D structure of the dendritic network. Since both the
topology and the connection strength are integral parts of the brain's wiring
diagram, being able to combine these two modalities is critically important.
In fact, these microscopes now routinely produce high-resolution imagery in
such large quantities that the bottleneck becomes automated processing and
interpretation, which is needed for such data to be exploited to its full
potential. In this paper, we briefly review the Computer Vision techniques we
have developed at EPFL to address this need. They include delineating dendritic
arbors from LM imagery, segmenting organelles from EM, and combining the two
into a consistent representation
A Pipeline for Volume Electron Microscopy of the Caenorhabditis elegans Nervous System.
The "connectome," a comprehensive wiring diagram of synaptic connectivity, is achieved through volume electron microscopy (vEM) analysis of an entire nervous system and all associated non-neuronal tissues. White et al. (1986) pioneered the fully manual reconstruction of a connectome using Caenorhabditis elegans. Recent advances in vEM allow mapping new C. elegans connectomes with increased throughput, and reduced subjectivity. Current vEM studies aim to not only fill the remaining gaps in the original connectome, but also address fundamental questions including how the connectome changes during development, the nature of individuality, sexual dimorphism, and how genetic and environmental factors regulate connectivity. Here we describe our current vEM pipeline and projected improvements for the study of the C. elegans nervous system and beyond
AxonDeepSeg: automatic axon and myelin segmentation from microscopy data using convolutional neural networks
Segmentation of axon and myelin from microscopy images of the nervous system
provides useful quantitative information about the tissue microstructure, such
as axon density and myelin thickness. This could be used for instance to
document cell morphometry across species, or to validate novel non-invasive
quantitative magnetic resonance imaging techniques. Most currently-available
segmentation algorithms are based on standard image processing and usually
require multiple processing steps and/or parameter tuning by the user to adapt
to different modalities. Moreover, only few methods are publicly available. We
introduce AxonDeepSeg, an open-source software that performs axon and myelin
segmentation of microscopic images using deep learning. AxonDeepSeg features:
(i) a convolutional neural network architecture; (ii) an easy training
procedure to generate new models based on manually-labelled data and (iii) two
ready-to-use models trained from scanning electron microscopy (SEM) and
transmission electron microscopy (TEM). Results show high pixel-wise accuracy
across various species: 85% on rat SEM, 81% on human SEM, 95% on mice TEM and
84% on macaque TEM. Segmentation of a full rat spinal cord slice is computed
and morphological metrics are extracted and compared against the literature.
AxonDeepSeg is freely available at https://github.com/neuropoly/axondeepsegComment: 14 pages, 7 figure
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