Robust particle outline extraction and its application to digital on-line holography

Peer reviewedPostprin

The ePetri dish, an on-chip cell imaging platform based on subpixel perspective sweeping microscopy (SPSM)

We report a chip-scale lensless wide-field-of-view microscopy imaging technique, subpixel perspective sweeping microscopy, which can render microscopy images of growing or confluent cell cultures autonomously. We demonstrate that this technology can be used to build smart Petri dish platforms, termed ePetri, for cell culture experiments. This technique leverages the recent broad and cheap availability of high performance image sensor chips to provide a low-cost and automated microscopy solution. Unlike the two major classes of lensless microscopy methods, optofluidic microscopy and digital in-line holography microscopy, this new approach is fully capable of working with cell cultures or any samples in which cells may be contiguously connected. With our prototype, we demonstrate the ability to image samples of area 6 mm × 4 mm at 660-nm resolution. As a further demonstration, we showed that the method can be applied to image color stained cell culture sample and to image and track cell culture growth directly within an incubator. Finally, we showed that this method can track embryonic stem cell differentiations over the entire sensor surface. Smart Petri dish based on this technology can significantly streamline and improve cell culture experiments by cutting down on human labor and contamination risks

An approach for real world data modelling with the 3D terrestrial laser scanner for built environment

Capturing and modelling 3D information of the built environment is a big challenge. A number of techniques and technologies are now in use. These include EDM, GPS, and photogrammetric application, remote sensing and traditional building surveying applications. However, use of these technologies cannot be practical and efficient in regard to time, cost and accuracy. Furthermore, a multi disciplinary knowledge base, created from the studies and research about the regeneration aspects is fundamental: historical, architectural, archeologically, environmental, social, economic, etc. In order to have an adequate diagnosis of regeneration, it is necessary to describe buildings and surroundings by means of documentation and plans. However, at this point in time the foregoing is considerably far removed from the real situation, since more often than not it is extremely difficult to obtain full documentation and cartography, of an acceptable quality, since the material, constructive pathologies and systems are often insufficient or deficient (flat that simply reflects levels, isolated photographs,..). Sometimes the information in reality exists, but this fact is not known, or it is not easily accessible, leading to the unnecessary duplication of efforts and resources. In this paper, we discussed 3D laser scanning technology, which can acquire high density point data in an accurate, fast way. Besides, the scanner can digitize all the 3D information concerned with a real world object such as buildings, trees and terrain down to millimetre detail Therefore, it can provide benefits for refurbishment process in regeneration in the Built Environment and it can be the potential solution to overcome the challenges above. The paper introduce an approach for scanning buildings, processing the point cloud raw data, and a modelling approach for CAD extraction and building objects classification by a pattern matching approach in IFC (Industry Foundation Classes) format. The approach presented in this paper from an undertaken research can lead to parametric design and Building Information Modelling (BIM) for existing structures. Two case studies are introduced to demonstrate the use of laser scanner technology in the Built Environment. These case studies are the Jactin House Building in East Manchester and the Peel building in the campus of University Salford. Through these case studies, while use of laser scanners are explained, the integration of it with various technologies and systems are also explored for professionals in Built Environmen

Autonomous flight and remote site landing guidance research for helicopters

Automated low-altitude flight and landing in remote areas within a civilian environment are investigated, where initial cost, ongoing maintenance costs, and system productivity are important considerations. An approach has been taken which has: (1) utilized those technologies developed for military applications which are directly transferable to a civilian mission; (2) exploited and developed technology areas where new methods or concepts are required; and (3) undertaken research with the potential to lead to innovative methods or concepts required to achieve a manual and fully automatic remote area low-altitude and landing capability. The project has resulted in a definition of system operational concept that includes a sensor subsystem, a sensor fusion/feature extraction capability, and a guidance and control law concept. These subsystem concepts have been developed to sufficient depth to enable further exploration within the NASA simulation environment, and to support programs leading to the flight test

Reverse engineering in construction

Recently a great deal of research into construction IT has been completed, and this is ongoing to improve efficiency and quality in the construction sector. The new innovation of 3D laser scanning is aimed at being used to improve the efficiency and quality of construction projects, such as maintenance of buildings or group of buildings that are going to be renovated for new services. The 3D laser scanner will be integrated with other VR tools such as GIS solutions and workbench for visualisation, analysis and interaction with a building VR model. An integration strategy is proposed for an Ordnance Survey map of the area and 3D model created by means of the laser scanner. The integrated model will then be transferred to the VR workbench in order to visualise, interact and analyse the interested buildings on purpose

Structural health monitoring of wind turbine blades: acoustic source localization using wireless sensor networks

Structural health monitoring (SHM) is important for reducing the maintenance and operation cost of safety-critical components and systems in offshore wind turbines. This paper proposes an in situ wireless SHM system based on an acoustic emission (AE) technique. By using this technique a number of challenges are introduced due to high sampling rate requirements, limitations in the communication bandwidth, memory space, and power resources. To overcome these challenges, this paper focused on two elements: (1) the use of an in situ wireless SHM technique in conjunction with the utilization of low sampling rates; (2) localization of acoustic sources which could emulate impact damage or audible cracks caused by different objects, such as tools, bird strikes, or strong hail, all of which represent abrupt AE events and could affect the structural health of a monitored wind turbine blade. The localization process is performed using features extracted from aliased AE signals based on a developed constraint localization model. To validate the performance of these elements, the proposed system was tested by testing the localization of the emulated AE sources acquired in the field

An approach to ground based space surveillance of geostationary on-orbit servicing operations

AbstractOn Orbit Servicing (OOS) is a class of dual-use robotic space missions that could potentially extend the life of orbiting satellites by fuel replenishment, repair, inspection, orbital maintenance or satellite repurposing, and possibly reduce the rate of space debris generation. OOS performed in geostationary orbit poses a unique challenge for the optical space surveillance community. Both satellites would be performing proximity operations in tight formation flight with separations less than 500m making atmospheric seeing (turbulence) a challenge to resolving a geostationary satellite pair when viewed from the ground. The two objects would appear merged in an image as the resolving power of the telescope and detector, coupled with atmospheric seeing, limits the ability to resolve the two objects. This poses an issue for obtaining orbital data for conjunction flight safety or, in matters pertaining to space security, inferring the intent and trajectory of an unexpected object perched very close to one׳s satellite asset on orbit. In order to overcome this problem speckle interferometry using a cross spectrum approach is examined as a means to optically resolve the client and servicer׳s relative positions to enable a means to perform relative orbit determination of the two spacecraft. This paper explores cases where client and servicing satellites are in unforced relative motion flight and examines the observability of the objects. Tools are described that exploit cross-spectrum speckle interferometry to (1) determine the presence of a secondary in the vicinity of the client satellite and (2) estimate the servicing satellite׳s motion relative to the client. Experimental observations performed with the Mont Mégantic 1.6m telescope on co-located geostationary satellites (acting as OOS proxy objects) are described. Apparent angular separations between Anik G1 and Anik F1R from 5 to 1 arcsec were observed as the two satellites appeared to graze one another. Data reduction using differential angular measurements derived from speckle images collected by the 1.6m telescope produced relative orbit estimates with better than 90m accuracy in the cross-track and in-track directions but exhibited highly variable behavior in the radial component from 50 to 1800m. Simulations of synthetic tracking data indicated that the radial component requires approximately six hours of tracking data for an Extended Kalman Filter to converge on an relative orbit estimate with less than 100m overall uncertainty. The cross-spectrum approach takes advantage of the Fast Fourier Transform (FFT) permitting near real-time estimation of the relative orbit of the two satellites. This also enables the use of relatively larger detector arrays (>106 pixels) helping to ease acquisition process to acquire optical angular data

Parametric high resolution techniques for radio astronomical imaging

The increased sensitivity of future radio telescopes will result in requirements for higher dynamic range within the image as well as better resolution and immunity to interference. In this paper we propose a new matrix formulation of the imaging equation in the cases of non co-planar arrays and polarimetric measurements. Then we improve our parametric imaging techniques in terms of resolution and estimation accuracy. This is done by enhancing both the MVDR parametric imaging, introducing alternative dirty images and by introducing better power estimates based on least squares, with positive semi-definite constraints. We also discuss the use of robust Capon beamforming and semi-definite programming for solving the self-calibration problem. Additionally we provide statistical analysis of the bias of the MVDR beamformer for the case of moving array, which serves as a first step in analyzing iterative approaches such as CLEAN and the techniques proposed in this paper. Finally we demonstrate a full deconvolution process based on the parametric imaging techniques and show its improved resolution and sensitivity compared to the CLEAN method.Comment: To appear in IEEE Journal of Selected Topics in Signal Processing, Special issue on Signal Processing for Astronomy and space research. 30 page