Learning to Generate Time-Lapse Videos Using Multi-Stage Dynamic Generative Adversarial Networks

Taking a photo outside, can we predict the immediate future, e.g., how would the cloud move in the sky? We address this problem by presenting a generative adversarial network (GAN) based two-stage approach to generating realistic time-lapse videos of high resolution. Given the first frame, our model learns to generate long-term future frames. The first stage generates videos of realistic contents for each frame. The second stage refines the generated video from the first stage by enforcing it to be closer to real videos with regard to motion dynamics. To further encourage vivid motion in the final generated video, Gram matrix is employed to model the motion more precisely. We build a large scale time-lapse dataset, and test our approach on this new dataset. Using our model, we are able to generate realistic videos of up to $128\times 128$ resolution for 32 frames. Quantitative and qualitative experiment results have demonstrated the superiority of our model over the state-of-the-art models.Comment: To appear in Proceedings of CVPR 201

Deep learning approach to Fourier ptychographic microscopy

Convolutional neural networks (CNNs) have gained tremendous success in solving complex inverse problems. The aim of this work is to develop a novel CNN framework to reconstruct video sequences of dynamic live cells captured using a computational microscopy technique, Fourier ptychographic microscopy (FPM). The unique feature of the FPM is its capability to reconstruct images with both wide field-of-view (FOV) and high resolution, i.e. a large space-bandwidth-product (SBP), by taking a series of low resolution intensity images. For live cell imaging, a single FPM frame contains thousands of cell samples with different morphological features. Our idea is to fully exploit the statistical information provided by these large spatial ensembles so as to make predictions in a sequential measurement, without using any additional temporal dataset. Specifically, we show that it is possible to reconstruct high-SBP dynamic cell videos by a CNN trained only on the first FPM dataset captured at the beginning of a time-series experiment. Our CNN approach reconstructs a 12800×10800 pixel phase image using only ∼25 seconds, a 50× speedup compared to the model-based FPM algorithm. In addition, the CNN further reduces the required number of images in each time frame by ∼ 6×. Overall, this significantly improves the imaging throughput by reducing both the acquisition and computational times. The proposed CNN is based on the conditional generative adversarial network (cGAN) framework. We further propose a mixed loss function that combines the standard image domain loss and a weighted Fourier domain loss, which leads to improved reconstruction of the high frequency information. Additionally, we also exploit transfer learning so that our pre-trained CNN can be further optimized to image other cell types. Our technique demonstrates a promising deep learning approach to continuously monitor large live-cell populations over an extended time and gather useful spatial and temporal information with sub-cellular resolution.We would like to thank NVIDIA Corporation for supporting us with the GeForce Titan Xp through the GPU Grant Program. (NVIDIA Corporation; GeForce Titan Xp through the GPU Grant Program)First author draf

Deep learning approach to Fourier ptychographic microscopy

Convolutional neural networks (CNNs) have gained tremendous success in solving complex inverse problems. The aim of this work is to develop a novel CNN framework to reconstruct video sequence of dynamic live cells captured using a computational microscopy technique, Fourier ptychographic microscopy (FPM). The unique feature of the FPM is its capability to reconstruct images with both wide field-of-view (FOV) and high resolution, i.e. a large space-bandwidth-product (SBP), by taking a series of low resolution intensity images. For live cell imaging, a single FPM frame contains thousands of cell samples with different morphological features. Our idea is to fully exploit the statistical information provided by this large spatial ensemble so as to make predictions in a sequential measurement, without using any additional temporal dataset. Specifically, we show that it is possible to reconstruct high-SBP dynamic cell videos by a CNN trained only on the first FPM dataset captured at the beginning of a time-series experiment. Our CNN approach reconstructs a 12800X10800 pixels phase image using only ~25 seconds, a 50X speedup compared to the model-based FPM algorithm. In addition, the CNN further reduces the required number of images in each time frame by ~6X. Overall, this significantly improves the imaging throughput by reducing both the acquisition and computational times. The proposed CNN is based on the conditional generative adversarial network (cGAN) framework. Additionally, we also exploit transfer learning so that our pre-trained CNN can be further optimized to image other cell types. Our technique demonstrates a promising deep learning approach to continuously monitor large live-cell populations over an extended time and gather useful spatial and temporal information with sub-cellular resolution

Alternating Back-Propagation for Generator Network

This paper proposes an alternating back-propagation algorithm for learning the generator network model. The model is a non-linear generalization of factor analysis. In this model, the mapping from the continuous latent factors to the observed signal is parametrized by a convolutional neural network. The alternating back-propagation algorithm iterates the following two steps: (1) Inferential back-propagation, which infers the latent factors by Langevin dynamics or gradient descent. (2) Learning back-propagation, which updates the parameters given the inferred latent factors by gradient descent. The gradient computations in both steps are powered by back-propagation, and they share most of their code in common. We show that the alternating back-propagation algorithm can learn realistic generator models of natural images, video sequences, and sounds. Moreover, it can also be used to learn from incomplete or indirect training data