Intestinal Absorption

This is the edited transcript of a Witness Seminar held at the Wellcome Institute for the History of Medicine,in London, on 9 February 1999. First published by the Wellcome Trust, 2000. ©The Trustee of the Wellcome Trust, London, 2000.All volumes are freely available online at: www.history.qmul.ac.uk/research/modbiomed/wellcome_witnesses/Annotated and edtied transcript of a Witness Seminar held on 9 February 1999. Introduction by Sir Christopher Booth.Annotated and edtied transcript of a Witness Seminar held on 9 February 1999. Introduction by Sir Christopher Booth.Annotated and edtied transcript of a Witness Seminar held on 9 February 1999. Introduction by Sir Christopher Booth.Annotated and edtied transcript of a Witness Seminar held on 9 February 1999. Introduction by Sir Christopher Booth.A record of a meeting chaired by Lord Turnberg that brought together those from laboratory research and medical practice to discuss some of the key aspects of intestinal absorption, including work on basic physiological mechanisms and techniques, such as the discovery of dedicated transport systems and their localization, and their clinical impact in intestinal disorders and oral rehydration therapy. Participants include: Sir Christopher Booth, Dr Richard Boyd, Professor Ramsey Bronk, Professor Hermon Dowling, Professor Michael Gardner, Dr Michael Hellier, Dr Roy Levin, Professor Richard Naftalin, Professor Timothy Peters, Professor John Walker-Smith and Professor Oliver Wrong. Christie D A, Tansey E M. (eds) (2000) Intestinal absorption, Wellcome Witnesses to Twentieth Century Medicine, vol. 8. London: The Wellcome Trust.The Wellcome Trust is a registered charity, no. 210183

Chapter Five. Systematic review results by biomarker classifications

5.1 Markers of Absorption and Permeability Overview 5.2 Markers of Absorption 5.3 Markers of Permeability 5.4 Markers of Digestion 5.5 Markers of Intestinal Inflammation and Intestinal Immune Activation 5.6 Markers of Systemic Inflammation and Systemic Immune Activation 5.7 Markers of Microbial Drivers 5.8 Markers of Nonspecific Intestinal Injury 5.9 Markers of Extra-Small Intestinal Function 5.10 Relationships Between Markers of EED, Including Histopathology 5.11 Relationships between EED Biomarkers and Growth or Other Outcomes of Interesthttps://digitalcommons.wustl.edu/tropicalenteropathybook/1006/thumbnail.jp

Low zinc status and absorption exist in infants with jejunostomies or ileostomies which persists after intestinal repair.

There is very little data regarding trace mineral nutrition in infants with small intestinal ostomies. Here we evaluated 14 infants with jejunal or ileal ostomies to measure their zinc absorption and retention and biochemical zinc and copper status. Zinc absorption was measured using a dual-tracer stable isotope technique at two different time points when possible. The first study was conducted when the subject was receiving maximal tolerated feeds enterally while the ostomy remained in place. A second study was performed as soon as feasible after full feeds were achieved after intestinal repair. We found biochemical evidence of deficiencies of both zinc and copper in infants with small intestinal ostomies at both time points. Fractional zinc absorption with an ostomy in place was 10.9% ± 5.3%. After reanastamosis, fractional zinc absorption was 9.4% ± 5.7%. Net zinc balance was negative prior to reanastamosis. In conclusion, our data demonstrate that infants with a jejunostomy or ileostomy are at high risk for zinc and copper deficiency before and after intestinal reanastamosis. Additional supplementation, especially of zinc, should be considered during this time period

Absorption of thiamine and nicotinic acid in the rat intestine during fasting and immobilization stress

By perfusion of isolated sections of intestine with a solution containing thiamine at a concentration of 3.1 micromole, it was established that thiamine absorption in animals fasted for 72 hours decreased by 28 percent, whereas absorption increased by 12 percent in rats after 24 hour immobilization. After immobilization, absorption of label in the intestinal mucosa increased. Na K ATPase activity in the intestinal mucosa decreased by 10 percent during fasting, and it increased with immobilization of the animals. Activity of Na K ATPase in the intestinal mucosa cells determined the absorption rate of thiamine and nicotinic acid at the level of vitamin transport through the plasma membranes of the enterocytes

Anthocyanin absorption and metabolism by human intestinal Caco-2 cells: a review

Anthocyanins from different plant sources have been shown to possess health beneficial effects against a number of chronic diseases. To obtain any influence in a specific tissue or organ, these bioactive compounds must be bioavailable, i.e., effectively absorbed from the gut into the circulation and transferred to the appropriate location within the body while still maintaining their bioactivity. One of the key factors affecting the bioavailability of anthocyanins is their transport through the gut epithelium. The Caco-2 cell line, a human intestinal epithelial cell model derived from a colon carcinoma, has been proven to be a good alternative to animal studies for predicting intestinal absorption of anthocyanins. Studies investigating anthocyanin absorption by Caco-2 cells report very low absorption of these compounds. However, the bioavailability of anthocyanins may be underestimated since the metabolites formed in the course of digestion could be responsible for the health benefits associated with anthocyanins. In this review, we critically discuss recent findings reported on the anthocyanin absorption and metabolism by human intestinal Caco-2 cells

The in vitro assessment of the bioavailability of iron in New Zealand beef : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Physiology at Massey University, Palmerston North, New Zealand /

The bioavailability of iron in New Zealand beef either alone or as part of a 'typical' New Zealand meal was investigated. The solubility of iron and its in vitro absorption by mouse intestinal tissue were used to evaluate iron bioavailability. The solubility of haem and/or non-haem iron in meat (beef longissimus muscle), vegetables and meat-plus-vegetables was investigated. Samples were cooked and then subjected to in vitro gastrointestinal digestion with pepsin followed by a combination of pancreatic enzymes and bile. Cooking at 65°C for 90 minutes reduced the soluble iron concentration in meat by 81% and reduced the haem iron concentration by 27%, which coincided with a 175% increase in non-haem iron concentrations. However, gastrointestinal digestion increased the solubility of iron in cooked meat (333%), vegetables (367%) and meat-plus-vegetables (167%). A proportion (35%) of the haem iron in the meat was broken down by the action of pancreatic enzymes leading to a 46% increase in non-haem iron concentrations, although this was not the case for the meat-plus-vegetables. Validation studies showed that mouse intestinal segments mounted in Ussing chambers maintained integrity and viability, and were responsive to glucose, theophylline and carbachol. Intestinal tissue from iron deficient mice was then used in the Ussing chambers to investigate the absorption of iron from ferrous gluconate and the soluble fractions of meat, vegetables and meat-plus-vegetables after gastrointestinal digestion. Results indicated a trend towards a higher absorption of iron from meat and ferrous gluconate, compared to vegetables and meat-plus-vegetables. However, iron absorption results were difficult to interpret due to the wide variation in the data. This variation was possibly due to errors associated with the sample processing and the analysis of iron, which was by inductively coupled-mass spectroscopy. Overall, the present study showed that before estimations can be made on the bioavailability of food iron, the effects of the cooking and gastrointestinal digestion processes must be considered. Further, the use of in vitro gastrointestinal digestion followed by the use of Ussing chambers to assess intestinal absorption is a potentially valuable system for assessing mineral bioavailability

Iron metabolism of in­testinal mucosa in various blood diseases

For the investigation of iron metabolism in the intestinal mucosa in various&#12288;blood diseases, intestinal biopsy (duodenum) was performed on 10 healthy controls&#12288;and 35 cases with various blood diseases. The following are&#12288;the results&#12288;of the studies on distribution of stainable&#12288;iron, amounts of non-hemin iron in the&#12288;biopsied materials, and iron uptake of the intestinal epithelial cells.&#12288;1) An evaluation of distribution of stainable iron by Berlin&#12288;blue reaction&#12288;showed none or very mild degree, if any,&#12288;inhealthy controls, an increase in&#12288;aplastic anemia,&#12288;pernicious anemia, some of leukemias and in iron deficiency anemia following iron therapy, and a decrease in&#12288;idiopathic hypochromic anemia, anchylostomiasis anemia,&#12288;anemia with cancer, myxedema, hemolytic anemia,&#12288;and in&#12288;some of leukemias. Some of anemia with cancer, however,&#12288;showed an&#12288;increase of a certain degree. In iron absorption tests, no changes were found other than a very mild increase in aplastic anemia. 2) Non-hemin iron was 70-112&#947;/g in healthy controls, increased in aplastic anemia approximately to 100-200&#947;/g, ranging 40-130&#947;/g in leukemia, and decreased in idiopathic hypochromic anemia and in anemia with cancer ranging 30-60&#947;/g and 30-50&#947;/g respectively. Amounts of non-hemin iron and serum iron or sideroblasts show a fair correlation. The fractionation of nonhemin iron in aplastic anemia didn't show any difference in relationship of each fraction from healthy controls despite the increased amount in the former. 3) A radioautographic evaluation of iron uptake by intestinal epithelium was performed by our device for evaluation of intestinal absorption capacity. The iron uptake was mild in healthy controls, almost none in aplastic anemia, and marked in iron deficiency anemia where it was decreased approximately to the level of healthy controls following iron therapy. 4) The intestinal tissue iron showed a series of changes similar to those of iron present in the serum or erythroblasts, and the non-hemin iron in the intestinal mucosa is inversely correlated with iron uptake of epithelium and is considered to regulate the absorption according to its amount.</p